Mouse IgG Fc Isotype Control 31205 from Thermo Fisher Scientific

Product Specs
ItemMouse IgG Fc Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number31205
Quantity1 mg
ApplicationsControl, Immunocytochemistry, Immunohistochemistry, Inhibition Assays
Concentration2.5 mg/mL
Conjugate/TagUnconjugated
FormatPBS with no preservative; pH 7.6
TargetMouse IgG Fc
IsotypeIgG
HostMouse
Storage Temperature4° C
Antibody TypeIsotype Control
Storage4°C
ClonalityPolyclonal

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Regional immune mechanisms enhance efficacy of an autologous cellular cancer vaccine with intraperitoneal administrationOncoImmunologyDecember 31, 2024Ben Marwedel, Lorél Y. Medina, Henning De May, Joshua E. Adogla, Ellie Kennedy, Erica Flores, Eunju Lim, Sarah Adams, Eric Bartee, Rita E. SerdaCD49d (R1–2) PerCP-eFluor 710 or PE, Fc receptor blockers (anti-CD16/CD32 (clone 2.4G2)), mouse IgG (31205), and LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit for 405 nm excitation were purchased from eBioscience™/Thermo Fisher Scientific

(2) Regional immune mechanisms enhance efficacy of an autologous cellular cancer vaccine with intraperitoneal administrationOncoimmunologyNovember 1, 2024Ben Marwedel, Lorél Y. Medina, Henning De May, Joshua E. Adogla, Ellie Kennedy, Erica Flores, Eunju Lim, Sarah Adams, Eric Bartee, Rita E. SerdaCD49d (R1–2) PerCP-eFluor 710 or PE, Fc receptor blockers (anti-CD16/CD32 (clone 2.4G2)), mouse IgG (31205), and LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit for 405 nm excitation were purchased from eBioscience™/Thermo Fisher Scientific

(3) Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myelomanpj Precision OncologySeptember 18, 2023Sonia M. Setayesh, Libere J. Ndacayisaba, Kate E. Rappard, Valerie Hennes, Luz Yurany Moreno Rueda, Guilin Tang, Pei Lin, Robert Z. Orlowski, David E. Symer, Elisabet E. Manasanch, et al.prior to staining. Slides were then blocked with a buffer containing 1% BSA and IgG mouse Fc fragments (31205, Thermo Scientific, Waltham, MA) for 1 h and then incubated with the antibody cocktail for 1.5 hr on

(4) Monitoring Therapeutic Responses to Silicified Cancer Cell Immunotherapy Using PET/MRI in a Mouse Model of Disseminated Ovarian CancerInternational Journal of Molecular SciencesSeptember 10, 2022Erik N. Taylor, Colin M. Wilson, Stefan Franco, Henning De May, Lorél Y. Medina, Yirong Yang, Erica B. Flores, Eric Bartee, Reed G. Selwyn, Rita E. Serda(Ox110) PerCP-eFlour 700. Antibodies, Fc receptor blockers (anti-CD16/CD32 (clone 2.4G2)), mouse IgG (31205), LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, and Far-Red Cell Stain Kit were purchased from eBioscience™/Thermo

(5) Cancer vaccines from cryogenically silicified tumour cells functionalized with pathogen-associated molecular patternsNature biomedical engineeringJanuary 1, 2022Guo J, De May H, Franco S, Noureddine A, Tang L, Brinker CJ, Kusewitt DF, Adams SF, Serda RE.710 (MP6-XT22), CD45R (B220) FITC, Fc receptor blockers (anti-CD16/CD32 (clone 2.4G2)), mouse IgG (31205), and LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit for 405 nm excitation were purchased from eBioscience™/Thermo Fisher Scientific

(6) Dynamic changes in the regulatory T-cell heterogeneity and function by murine IL-2 muteinLife science allianceMay 1, 2020Lu DR, Wu H, Driver I, Ingersoll S, Sohn S, Wang S, Li CM, Phee H.IL-2M on T-cell proliferation, Ki67 staining was performed. Untreated, PBS- or mouse IgG Fc isotype (31205; Thermo Fisher Scientific)–treated mice were compared and used as negative controls. Administration

(7) An engineered oncolytic virus expressing PD-L1 inhibitors activates tumor neoantigen-specific T cell responsesNature communicationsMarch 13, 2020Wang G, Kang X, Chen KS, Jehng T, Jones L, Chen J, Huang XF, Chen SY.before the experiment. On the day of experiment, different amounts of PBS, IgG Fc (Thermo, cat. no.: 31205) or purified iPDL1 were added into wells containing target MC38 cells followed by the addition of 6

(8) Senescent hepatocytes enhance natural killer cell activity via the CXCL-10/CXCR3 axisExperimental and therapeutic medicineNovember 1, 2019Zang J, Ye J, Zhang C, Sha M, Gao J.washed with and resuspended in ice-cold PBS. Fc receptors were blocked with 30 mg/ml mouse IgG (cat. no. 31205; Invitrogen; Thermo Fisher Scientific, Inc.) and 10 mg/ml anti-CD16/32 (cat. no. 101302; BioLegend, Inc

(9) Sema3a-Nrp1 Signaling Mediates Fast-Twitch Myofiber Specificity of Tw2⁺ CellsDevelopmental cellOctober 7, 2019Li S, Karri D, Sanchez-Ortiz E, Jaichander P, Bassel-Duby R, Liu N, Olson EN.then washed three times with PBS followed by addition of 10 µg of control Fc fragments (Invitrogen, 31205) in 200 µl of PBS on top of the stripes as a counter coating for 30 mins at 37 °C. The dish was washed

(10) Tumor cells induce LAMP2a expression in tumor-associated macrophage for cancer progressionEBioMedicineFebruary 1, 2019Wang R, Liu Y, Liu L, Chen M, Wang X, Yang J, Gong Y, Ding BS, Wei Y, Wei X.cells were incubated with live/dead staining dye (Invitrogen, L34974) and FcR blocking (Invitrogen, 31205) for 15 min in 4 °C. Then cells were stained by antibodies following per corresponding protocol. For

(11) A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodiesThe Journal of cell biologyMarch 5, 2018Pleiner T, Bates M, Görlich D.(401302; BioLegend); polyclonal IgG Fab fragments (010-0105; Rockland); and polyclonal IgG Fc fragments (31205; Thermo Fisher Scientific). Polyclonal IgG of the following species were used: rabbit (made in-house,

(12) Clonal diversity revealed by morphoproteomic and copy number profiles of single prostate cancer cells at diagnosisConvergent Science Physical OncologyMarch 1, 2018Paymaneh D Malihi et al.Slides were blocked using 1% BSA with 0.2 mg ml−1 mouse IgG Fc fragment (Thermofisher, Waltham, USA, Cat#31205) in PBS for 60 min at 37 °C. The MaxPar™ metal-labeled antibody cocktail was prepared in 0.1% Tween

(13) Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1BJournal of neuroinflammationMarch 12, 2015Klein D, Groh J, Weishaupt A, Martini R.limpet hemocyanin (KLH)-antibody (orb11067, Biorbyt, Cambridge, UK) or 50 μg mouse IgG Fc fragments (31205, Pierce, Rockford, IL, USA) were injected into the tail vein (i.v.) once or at four consecutive weeks

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Mouse IgG Fc Isotype Control from Thermo Fisher Scientific

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Mouse IgG Fc Isotype Control from Thermo Fisher Scientific

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