eIF4GI Antibody 2858S from Cell Signaling Technology

Product Specs
ItemeIF4GI Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2858S
Quantity100 µl
ApplicationsW, IHC-P, IF-IC
ReactivityH, M, R
TargeteIF4G
HostRabbit
NCBI Gene AliasesEIF-4G1, EIF4F, EIF4G, EIF4GI, P220, PARK18
NCBI Full Gene Nameeukaryotic translation initiation factor 4 gamma 1
ClonalityPolyclonal
Research AreaTranslational Control
Molecular Weight220

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Eukaryotic initiation factors eIF4F and eIF4B promote translation termination upon closed-loop formationNucleic Acids ResMarch 11, 2025Ekaterina Shuvalova, Alexey Shuvalov, Walaa Al Sheikh, Alexander V Ivanov, Nikita Biziaev, Tatiana V Egorova, Sergey E Dmitriev, Ilya M Terenin, Elena Alkalaevawestern blotting with corresponding antibodies raised against eIF4G1 (Cell Signaling, Danvers, USA, 2858S), eIF4A (Cell Signaling, Danvers, USA, 2013S), eRF1 (Abcam, Cambridge, UK, 153731), eRF3 (Cell Signaling,

(2) Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cellsiScienceJuly 19, 2024Mingchao Jiang, Ning Wang, Yuqin Zhang, Jinjin Zhang, Youwei Li, Xiu Yan, Honghao Zhang, Chengbin Li, Youfei Guan, Bin Liang, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, p-AKT,Proteintech,CAT:28731-1-AP AKT,Proteintech,CAT:10176-2-AP Proinsulin,Abcam,CAT:ab243141 Insulin,Abclonal,CAT:A19066 PC1/3,Abcam,CAT:ab220363 PCSK2,Proteintech,CAT:10553-1-AP CPE,Santa Cruz Biotechnology,CAT:sc-393761 eIF4G1,CST,CAT:2858 p-eIF2α,CST,CAT:9721 eIF2α,CST,CAT:9722 Pdx1,Abclonal,CAT:A10173 SREBP1,Abcam,CAT:ab28481 p-mTOR,CST,CAT:2971 mTOR,Proteintech,CAT:28273-1-AP p-p70 S6K

(3) Phosphorylation of enteroviral 2A(pro) at Ser/Thr125 benefits its proteolytic activity and viral pathogenesisJournal of medical virologyJanuary 1, 2023Wang Y, Zou W, Niu Y, Wang S, Chen B, Xiong R, Zhang P, Luo Z, Wu Y, Fan C, et al.as follows: phosphothreonine (p‐Thr, ab9337; Abcam), phosphoserine (p‐Ser, ab9332; Abcam), eIF4GI (2858s; Cell Signaling Technology), MDA5 (5321s; Cell Signaling Technology), GFP (2955s; Cell Signaling Technology),

(4) Human dyskerin binds to cytoplasmic H/ACA-box-containing transcripts affecting nuclear hormone receptor dependenceGenome biologyAugust 22, 2022Zacchini F, Venturi G, De Sanctis V, Bertorelli R, Ceccarelli C, Santini D, Taffurelli M, Penzo M, Treré D, Inga A, et al.anti-RPS14 (Santa Cruz Biotechnology, sc-68873), anti-RPL5 (Bethyl, A303-933A), anti-EIF4GI (Cell Signaling, 2858S) anti- puromycin (Merk, MABE343). Polysome fractionation For standard polysome fractioning, cultured

(5) Engineering an autonomous VH domain to modulate intracellular pathways and to interrogate the eIF4F complexNature CommunicationsAugust 18, 2022Yuri Frosi, Yen-Chu Lin, Jiang Shimin, Siti Radhiah Ramlan, Kelly Hew, Alf Henrik Engman, Anil Pillai, Kit Yeung, Yue Xiang Cheng, Tobias Cornvik, et al.signalling Technology,1:1000 Anti AKT,,Not provided,Cell signalling Technology,1:1000 Anti eiF4G1,2858,Not provided,Cell signalling Technology,1:500 Anti cyclin D1,2988,92G2,Cell signalling Technology,1:500 Anti

(6) Localization of a TORC1-eIF4F translation complex during CD8(+) T cell activation drives divergent cell fateMolecular cellJuly 7, 2022Liedmann S, Liu X, Guy CS, Crawford JC, Rodriguez DA, Kuzuoğlu-Öztürk D, Guo A, Verbist KC, Temirov J, Chen MJ, et al.ab31217, Abcam), anti-phospho-eIF4GIS1108 (1:500, 2441, Cell Signaling Technology), anti-eIF4GI (1:500, 2858, Cell Signaling Technology), anti-Mcl1 (1:500, 24296, Cell Signaling Technology), anti-phospho-mTORS2481

(7) Recognition of 3â€² nucleotide context and stop codon readthrough are determined during mRNA translation elongationJournal of Biological ChemistryJuly 1, 2022Nikita Biziaev, Elizaveta Sokolova, Dmitry V. Yanvarev, Ilya Yu Toropygin, Alexey Shuvalov, Tatiana Egorova, Elena Alkalaevawere analyzed by Western blotting using antibodies against eIF3a (Abcam 86146), eIF4GI (Cell Signaling 2858), His-tag (Miltenyi Biotec 130-092-785), and rpL9 (Abcam 182556). Readthrough efficiency analysis on

(8) Suppression of NLRP3 Inflammasome by Dihydroarteannuin via the HIF-1α and JAK3/STAT3 Signaling Pathway Contributes to Attenuation of Collagen-Induced Arthritis in MiceFrontiers in pharmacologyJanuary 1, 2022Zhang M, Wu D, Xu J, Liu L, Jiao W, Yu J, Chen G.Phospho-STAT3 (Cat. No. 9145), JAK3 (Cat. No. 8863), Phospho-JAK3 (Cat. No. 5031), and GAPDH (Cat. No. 2858) were purchased from Cell Signaling Technology. The primary antibodies against NLRP3 (Cat. No. PA5-79740)

(9) N6-Methyladenosine Methyltransferase METTL14-Mediated Autophagy in Malignant Development of Oral Squamous Cell CarcinomaFrontiers in OncologyNovember 24, 2021Fang Wang, Yue Zhu, Hongshi Cai, Jianfeng Liang, Wenjin Wang, Yan Liao, Yadong Zhang, Cheng Wang, Jinsong Hou(#86132), anti-WTAP (#41934), anti-FTO (#31687), anti-ALKBH5 (#80283), anti-LC3B (#4599), anti-eIF4G1 (#2858) (1:1 000, Cell Signaling Technology, Danvers, MA, USA), anti-METTL14 (#ab220030, 1:1 000, Abcam, Cambridge,

(10) Translocating lipopolysaccharide correlates with the severity of enterovirus A71-induced HFMD by promoting pro-inflammation and viral IRES activityGut pathogensNovember 22, 2021Wang Y, Dan K, Xue X, Yang X, Feng X, Yang Q, Yang J, Chen B.Abnova and EASYBIO, respectively. Anti-ERK1/2 (#9102), anti-phospho-ERK1/2 (#9101) and anti-eIF4GI (#2858) were purchased from Cell Signaling Technology. The target protein and β-actin were detected with anti-rabbit

(11) Targeting eIF4F translation complex sensitizes B-ALL cells to tyrosine kinase inhibitionScientific reportsNovember 4, 2021Vo TT, Herzog LO, Buono R, Lee JS, Mallya S, Duong MR, Thao J, Gotesman M, Fruman DA.the proteins of interest from two different origins (for eIF4G1, Cell signaling Technologies Cat. #2858, 1:200 dilution; for eIF4E, BD Biosciences Cat. #610269, 2.5 µg/ml final). Cells were next incubated

(12) Selective inhibitors of mTORC1 activate 4EBP1 and suppress tumor growthNature chemical biologyOctober 1, 2021Lee BJ, Boyer JA, Burnett GL, Thottumkara AP, Tibrewal N, Wilson SL, Hsieh T, Marquez A, Lorenzana EG, Evans JW, et al.anti-AKT (C67E7) #4691, anti-p-HER3 (Tyr1197) (C56E4) #4561, anti-HER3 (D22C5) #12708, anti-eIF4G1 #2858, anti-AKT #4691, anti-p-AKT (Ser473) #9271, anti-mTOR #2972, anti-β-actin (8H10D10) #3700, anti-HATag

(13) Targeting eIF4F translation initiation complex with SBI-756 sensitises B lymphoma cells to venetoclaxBritish Journal of CancerMarch 16, 2021Lee-or Herzog et al.followed by probing with primary antibodies for eIF4G1 (Cell signaling Technologies, Danvers, MA, Cat. #2858, 1:200 dilution) and eIF4E (BD Biosciences, San Diego, CA, Cat. #610269, 2.5 µg/ml final). Next, cells

(14) Pentamidine Inhibits Ovarian Cancer Cell Proliferation and Migration by Maintaining Stability of PTEN in vitroDrug design, development and therapyJanuary 1, 2021Wu Y, Zhang Z, Kou Z.MedChemExpress (Shanghai China). Cycloheximide (CHX) was obtained from Sigma-Aldrich (St. Louis, MO, USA). MG132 was 2858 Powered by TCPDF (www.tcpdf.org) https://doi.org/10.2147/DDDT.S311187 DovePress obtained from Cell Signaling Technology

(15) Upregulation of 5'-terminal oligopyrimidine mRNA translation upon loss of the ARF tumor suppressorScientific reportsDecember 17, 2020Cottrell KA, Chiou RC, Weber JD.Cell Signaling Technologies—TPT1(5128), p-MTOR (2971), p-S6 (2215S), p-S6K (9205S), p-4EBP1 (2855S), eIF4G1 (2858), p53 (2524S), eIF4E (9742S), 4EBP1 (9452S); Santa Cruz Biotechnology—ARF (sc-32748), RPL22 (sc-136413),

(16) A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactionsEuropean Journal of Medicinal ChemistryNovember 1, 2020Erin E. Gallagher, Arya Menon, Alyah F. Chmiel, Kirsten Deprey, Joshua A. Kritzer, Amanda L. GarnerChemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology. 4.2. Data and Statistical Analysis: All

(17) Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1): a target for cancer therapeutic intervention?Cancer Cell InternationalDecember 1, 2019Praveen Kumar Jaiswal, Sweaty Koul, Nallasivam Palanisamy, Hari K. Koulvivo using preclinical model systems. Material and methods Materials Antibody for EIF4G1 (Cell Signaling #2858, 1:1000 dilution) and GAPDH (Sigma G8795, 1:3000 dilution) were used to probe respective proteins of

(18) SG formation relies on eIF4GI-G3BP interaction which is targeted by picornavirus stress antagonistsCell DiscoveryDecember 1, 2019Xiaodan Yang, Zhulong Hu, Qiang Zhang, Shanshan Fan, Yi Zhong, Dong Guo, Yali Qin, Mingzhou ChenRabbit polyclonal anti-eIF4E (Cat#A2162) was purchased from ABclonal. Rabbit monoclonal anti-eIF4GI (Cat#2858 S) and rabbit monoclonal anti-DYKDDDDK (Flag; Cat#14793 S) were purchased from Cell Signaling Technology.

(19) SILAC-based quantitative mass spectrometry-based proteomics quantifies endoplasmic reticulum stress in whole HeLa cellsDisease Models & MechanismsNovember 1, 2019Daniel N. Itzhak, Francesca Sacco, Nagarjuna Nagaraj, Stefka Tyanova, Matthias Mann, Marta Murgiaincluding secondary antibodies used here), anti-MAFF (1:500; AV38984, Sigma-Aldrich), anti-eIF4G1 (1:1000; 2858S, Cell Signaling Technology), anti-tubulin (1:1000; sc-9104, Santa Cruz Biotechnology). Puromycin incorporation

(20) Consideration of Binding Kinetics in the Design of Stapled Peptide Mimics of the Disordered Proteins Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 and Eukaryotic Translation Initiation Factor 4GJournal of Medicinal ChemistryMay 23, 2019Erin E. Gallagher, James M. Song, Arya Menon, Lauren D. Mishra, Alyah F. Chmiel, Amanda L. Garnerreceived. Fmoc-(S)-2-(4-pentenyl)alanine-OH was purchased from Aldrich and used as received. eIF4E (9742), 4E-BP1 (9644), and eIF4G (2858) antibodies were purchased from Cell Signaling Technology. Data and Statistical Analysis. All data were

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eIF4GI Antibody

eIF4GI Antibody from Cell Signaling Technology

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Cell Signaling Technology

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eIF4GI Antibody from Cell Signaling Technology

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