Published customer image: Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the detection of macrophages in mouse lung and pancreas by immunohistochemistery on formalin fixed, paraffin tissue sections.Image caption:Changes in pancreatic and lung F4/80+ macrophage number during acute pancreatitis. Representative photomicrographs of pancreas (A) and lung (C) sections stained for F4/80 as indicator of macrophage infiltration (brown) 24 h after pancreatitis induction (original magnification, ×20). Quantification of F4/80+ cells showed a significantly increased enrichment in the pancreas from 9 h following pancreatitis compared to sham controls (B). No significant difference was observed in the number of lung F4/80+ cells between pancreatitis and sham control groups (D). Bars show mean ± SEM, n = 10 per group. **P<0.01, ***P<0.001, by two-tailed Student t-test.From: Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R (2012)Enrichment of Murine CD68+CCR2+ and CD68+CD206+ Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.PLoS ONE 7(10): e42654.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
RAT ANTI MOUSE F4/80:Biotin
Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 (MCA497A647) at a 1/5 dilution and CD11b at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock
Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 (MCA497A647) at a 1/5 dilution and CD11b (MCA74F) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock (BUF041B)
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