Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term) (Cat. #TA324606). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.

IF image of A431 cell stained with RCAN1 Antibody (N-term) (Cat#TA324606).A431 cells were incubated with RCAN1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). RCAN1 immunoreactivity is localized to Cytoplasm significantly.