Anti-mouse MIP-1a in WB shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated (0.1 ug, 7.8 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was incubated with Rockland's, Inc. Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight‚Ñ¢ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min.