WB using Anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head). Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 ug of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1, 700 dilution of anti-Ajuba antibody. Detection occurred using a 1:10,000 dilution of IRDye‚Ñ¢800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT.