IF image of Hela cell stained with EN2 Antibody (C-term) (Cat#TA324936). Hela cells were incubated with EN2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.