IF image of A549 cell stained with TBX21 Antibody (N-term) (Cat#TA324549).A549 cells were incubated with TBX21 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).TBX21 immunoreactivity is localized to Nucleus significantly.