WB using Anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein.The left panel shows a similar staining pattern using an anti-Flag‚Ñ¢ antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1, 400.