Lane 1: Recombinant hAHSP. Lane 2: Recombinant mAHSP. Lane 3: mice Spleen cells transfected with TNS9.2-hAHSP. Lane 4: mice Spleen cells transfected with TNS9.2 control vector. Lane 5: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by MACS using Ter119+ microbeads. Lane 6: mice Spleen cells transfected with TNS9.2 control vector fractionated by Ter119+. Lane 7: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by Ter119-. Lane 8: Spleen cells from mice transduced with TNS9.2 control vector fractionated by Ter119-. Load: 10ng per lane. Anti-AHSP: 1:1,000; Secondary: HRP Streptavidin at 1:40,000.
WB of Rabbit anti-AHSP antibody. Lane 1: Recombinant hAHSP (2 ng). Lane 2: Recombinant mAHSP (2 ng). Lane 3: RBC Lysates Mouse #24 - GFP. Lane 4: RBC Lysates Mouse #25 - GFP. Lane 5: RBC Lysates Mouse #16 - hAHSP. Lane 6: RBC Lysates Mouse #17 - hAHSP. Lane 7: RBC Lysates Mouse #22 - hAHSP. Lane 8: RBC Lysates Mouse #19 - hAHSP. Lane 9: RBC Lysates Mouse #20 - hAHSP. Primary antibody: hAHSP antibody, Beta-Actin antibody at 1:1,000. Secondary antibody:1:40,000.
IHC of Rabbit anti-AHSP antibody. Tissue: A. Normal bone marrow, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. C. AHSP stains nucleated EPs, but not mature, anucleate RBCs. D. CD71 stains nucleated EPs, but not mature, anucleate RBCs. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT).
IHC of Rabbit anti-AHSP antibody. Tissue: A. Acute erythroleukemia, H&E. B. CD235a stains erythroid blasts and mature, anucleate RBCs. C. AHSP stains erythroid blasts. D. CD71 stains erythroid blasts. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT)
IHC of Rabbit anti-AHSP antibody. Tissue: AHSP stains residual EPs and not myeloid blasts in acute myeloid leukemia with minimal differentiation (A), whereas CD71 stains both myeloid blasts and EPs (B). AHSP does not stain myeloid blasts in acute myelomonocytic leukemia (D), whereas CD71 does (E). C and F are corresponding H&Es, respectively. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT).
Supplier Page from OriGene Technologies for AHSP Rabbit Polyclonal Antibody