Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #TA324625) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified Pab.

Western blot analysis of anti-LC3 (APG8b) in untreated or treated Hela cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ( (APG8b) were detected in soluble fraction (S).

Wild-type (Cln3+/+) or homozygous Cln3 paraffin-embedded brain sections immunostained for the LC3 protein (Cat.# TA324625 LC3 antibody). Shown are the CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Few immunopositive puncta are present in wild-type sections, whereas homozygous Cln3 sections contain clusters of LC3-positive puncta around pyramidal neurons and Purkinje cells (P). MOL, molecular layer; GCL, granule cell layer.

Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B) (N-term) (Cat. #TA324625). U251 cells (right) were treated with Chloroquine (50 μM, 16h). TA324625 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).