Western blot using affinity purified anti-Smad3 antibody shows detection of endogenous Smad3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF-β lanes for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
Affinity Purified anti-Smad3 antibody shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, Uppsala, Sweden.
Supplier Page from OriGene Technologies for SMAD3 Rabbit Polyclonal Antibody