RAT ANTI MOUSE CD68 MCA1957T from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemRAT ANTI MOUSE CD68
CompanyBio-Rad
Price $110.00Supplier Page View Company Product Page
Catalog NumberMCA1957T
Quantity25 µg
ApplicationsWestern Blotting,Immunohistology - Paraffin,Immunoprecipitation,Flow Cytometry,Immunohistology - Frozen,Immunofluorescence
ConcentrationIgG concentration 1.0 mg/ml
FormatPurified IgG - liquid
ReactivityMouse
TargetCD68
IsotypeIgG2a
CloneFA-11
HostRat
ImmunogenPurified Concanavalin A acceptor glycoprotein from P815 cell line
NCBI Full Gene NameCD68 antigen
NCBI Gene Aliasesgp110, Lamp4, Scard1
ClonalityMonoclonal Antibody

Questions and Answers

What applications has the rat anti-mouse CD68 antibody been shown to work in?

The rat anti-mouse CD68 antibody has been shown to work in various applications including Western Blotting, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation.

Is there any evidence of the antibody's effectiveness in studying microglial activation in neurodegenerative diseases?

Yes, the antibody has been utilized in studies investigating microglial activation, such as in the context of amyotrophic lateral sclerosis, where it was used to characterize the colocalization of NLRP3 inflammasome components with microglial markers in spinal cord tissue of ALS mouse models ().

How has this antibody been applied in research related to diabetic retinopathy?

The rat anti-mouse CD68 antibody has been used to analyze macrophage infiltration in post-mortem donor eyes with a history of Type II diabetes, contributing to the understanding of inflammatory pathways involved in diabetic retinopathy ().

Can this antibody be used to assess macrophage presence in tumor microenvironments?

Yes, the antibody has been employed in studies examining the role of macrophages in tumor microenvironments, such as in breast cancer metastasis, where it was used to characterize macrophage populations in lung metastatic lesions ().

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) The microglial TREM2 receptor programs hippocampal development in a mouse model of childhood deprivationBrain, Behavior, and ImmunityAugust 1, 2026Sahabuddin Ahmed, Christian Bowers, Jose Munoz-Martin, Sumit Jamwal, Basavaraju G. Sanganahalli, Kyle Chen, Lauryn Giuliano, Zoë A. MacDowell Kaswan, Fahmeed Hyder, X. William Yang, et al.mouse anti-PSD95 (1:100; Merck-Millipore, Cat. #MAB1596), and rat anti-CD68 (1:400; Bio-Rad, Cat. # MCA1957T) overnight at 4°C in TBST-0.5% Triton X-100 with 3% NGS. TREM2 expression was determined using rabbit

(2) FGF2 Deficiency Modulates Early Microglial Responses Without Affecting Photoreceptor Survival in a Retinitis Pigmentosa Mouse ModelCellsApril 2, 2026Felia C. Haffelder, Nundehui Díaz-Lezama, Zeynep Okutan, Claudia Grothe, Susanne F. Koch#MABN2429), FGF2 (1:25; Merck, Darmstadt, Germany #05-118), CD68 (1:500, Biorad, Hercules, CA, USA #MCA1957T), VEGFA (1:500, CUSABIO, Houston, Texas, USA #CSB-PA08249A0) and glutamine synthetase (1:2000, Abcam,

(3) Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male miceBrain Behav Immun HealthMarch 3, 2026Janine Kox, Katherine Picard, Eva Šimončičová, Virginie Vidal, Mohammadparsa Khakpour, Sophia Wilhelm, Sophia Burns, Fernando González Ibáñez, Benneth Ben-Azu, Robbert Havekes, et al.antibodies against Iba1 (1:1000, rabbit, catalog: 019-19741, FUJIFILM Wako) and CD68 (1:500, rat, catalog: MCA1957T, Bio-Rad) in BB was added and incubated overnight at 4 °C. The next day, after 15 min at RT and washing

(4) Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosisImmunityFebruary 24, 2026Ana C. Codo, Jesus E. Romero-Pichardo, Zhaoquan Wang, Mariano A. Aufiero, Tomi Lazarov, Waleska Saitz Rojas, Nicole S. Walker, Achuth Nair, Roger F. Cole, Savannah Adkins, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rabbit anti-mouse Iba1,Wako Chemicals,Cat#011-27991 Rat anti-mouse CD68,Bio-Rad,Cat#MCA1957T Mouse anti-mouse MIS (B-11) P,Santa Cruz Biotechnology,Cat#sc-166752 P "Goat anti-rabbit IgG, Alexa

(5) Cell-intrinsic vulnerability and immune activation cooperate to drive degeneration in a mitochondrial complex I deficiency model of optic neuropathyJournal of NeuroinflammationFebruary 6, 2026Daniela Santamaría-Muñoz, Raenier V. Reyes, Miranda R. Krueger, Andrea García-Llorca, Brennan Marsh-Armstrong, Xin Duan, Yang Hu, Derek S. Welsbie, Nicholas Marsh-Armstrong, Elisenda Sanz, et al.(mouse),Biotechne,MAB150,1:50 Anti-CD3 FITC conjugate (rat),Cell Signaling Technology,86603,1:50 Anti-CD68 (rat),Biorad,MCA1957T,1:500 Anti-Cone Arrestin (rabbit),Millipore,Ab15282,1:400 Anti-GFAP (mouse),Sigma-Aldrich,G3893,1:500 Anti-Iba1

(6) Staphylococcus aureus exhibits spatiotemporal heterogeneity in Sae activity during kidney abscess developmentmBioDecember 10, 2025Anjali Anil, Rezia Era D. Braza, Bessie Liu, Valerie Altouma, Clement Adedeji, Ananyaa Welling, Alysha L. Ellison, Megan H. Check, Irnov Irnov, Rachel Prescott, et al.anti-mouse Ly6G antibody (Invitrogen: 17-9668-80) or rat anti-mouse CD68 primary antibody (BioRad: MCA1957T) diluted in 2% BSA overnight at 4°C. For CD68 detection, sections were washed in PBS and incubated with

(7) Development of a Modified Weight-Drop Apparatus for Closed-Skull, Repetitive Mild Traumatic Brain Injuries in a Mouse ModeleNeuroDecember 4, 2025Anthony B. Crum, Cara D. Nielson, Kaylin J. Sevilla Lopez, Julian P. Meekscerebellum). Immunostaining was performed using anti-CD68 (1:1,000, Bio-Rad Laboratories, catalog #MCA1957T) and anti-Iba1 (1:1,000, FUJIFILM Wako Pure Chemical, catalog #019-19741) primary antibodies. Secondary

(8) OPTN protects retinal ganglion cells and ameliorates neuroinflammation in optic neuropathiesCommun BiolOctober 16, 2025Qinglong Wang, Yiqi Wang, Yi Da Douglas Jiang, Ryan Donahue, Gaby Cao, Weixuan Yan, Hong Guo, Zhenghui Li, Jenna Liang, Jin Hao, et al.Cayman, No. 100,000, 1:100; RBPMS, Raygene, A008712, 1:500; Iba1, Novus, NB100-1028, 1:500; CD68, Biorad, MCA1957T, 1:500; GFAP, Dako, Z0334, 1:500) overnight at 4 °C. On the next day, the corresponding Alexa Fluor

(9) A co-culture system to study the effects of Poly I:C-activated microglia on the differentiation of murine primary neural stem cellsIn Vitro Cellular & Developmental Biology - AnimalSeptember 30, 2025Marie Pierre Manitz, Karina Violou, Malin Hedstück, Kimberly Bösing, Maria Kottmann, Nadja Freund, Georg JuckelIba1 (1:500, FUJIFILM Wako Chemicals, Neuss, Germany, 019–19,741), CD68 (1:500, Bio-Rad, Hercules, CA, MCA1957T), CX3CR1 (Thermo Fisher Scientific, Waltham, MA, 1:700, 14–6093), Ki-67 (1:100, Santa Cruz Biotechnology,

(10) The Microglial TREM2 Receptor Programs Hippocampal Development in a Mouse Model of Childhood DeprivationbioRxivAugust 11, 2025Sahabuddin Ahmed, Christian Bowers, Jose Munoz-Martin, Sumit Jamwal, Basavaraju G. Sanganahalli, Lauryn Giuliano, Zoë A. MacDowell MacDowell Kaswan, Fahmeed Hyder, X. William Yang, Arie Kaffmanmouse anti-PSD95 (1:100; Merck-Millipore, Cat. #MAB1596), and rat anti-CD68 (1:400; Bio-Rad, Cat. # MCA1957T) overnight at 4°C in TBST-0.5% Triton X-100 with 3% NGS. TREM2 expression was determined using rabbit

(11) Development of a modified weight-drop apparatus for closed-skull, repetitive mild traumatic brain injuries in a mouse modelbioRxivAugust 1, 2025Anthony Bryan Crum, Cara D. Nielson, Kaylin J. Sevilla Sevilla Lopez, Julian P. MeeksImmunostaining was performed using anti-CD68 (1:1000, Bio-Rad Laboratories, Hercules, CA, Cat. No. MCA1957T) and anti-Iba1 (1:1000, FUJIFILM Wako Pure Chemical Corp., Osaka, Japan, Cat. No. 019–19741) primary

(12) The myeloid cell-driven transdifferentiation of endothelial cells into pericytes promotes the restoration of BBB function and brain self-repair after strokeElifeJuly 16, 2025Tingbo Li, Ling Yang, Jiaqi Tu, Yufan Hao, Zhu Zhu, Yingjie Xiong, Qingzhu Gao, Lili Zhou, Guanglei Xie, Dongdong Zhang, et al.RRID:AB_11213099, RRID:AB_11213099), NG2 (1:300, Merck, Cat# AB5320, RRID:AB_11213100), CD68 (1:300, Bio-Rad, Cat# MCA1957T, RRID:AB_322219), CD45-APC (1:200, BioLegend, Cat# 103112, RRID:AB_312977), Ki67 (1:300, Thermo Scientific,

(13) GADD45G operates as a pathological sensor orchestrating reactive gliosis and neurodegenerationNeuronJuly 9, 2025Tianjin Shen, Wenjiao Tai, Dongfang Jiang, Shuaipeng Ma, Xiaoling Zhong, Yuhua Zou, Chun-Li Zhang1:1000; Abcam), IBA1 (019–19741; rabbit; 1:1000; Wako), IBA1 (NB100–1028; goat; 1:1000; Novus), CD68 (MCA1957T; rat; 1:1000; BioRad), NeuN (266 004; guinea pig; 1:500; Synaptic System), 6E10 amyloid plaques (803007;

(14) Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunityNatureJuly 1, 2025Faust Akl C, Andersen BM, Li Z, Giovannoni F, Diebold M, Sanmarco LM, Kilian M, Fehrenbacher L, Pernin F, Rone JM, et al.(Cell Signaling Technology, 9661T), rabbit anti-IL11RA1 (Abcam, ab125015) and rat anti-CD68 (Bio-Rad, MCA1957T). HSV-infected glioblastoma lines were imaged for GFP fluorescence using a Leica DMi8 Widefield microscope

(15) Plasmalogen remodeling modulates macrophage response to cytotoxic oxysterols and atherosclerotic plaque vulnerabilityCell Reports MedicineMay 20, 2025Antoine Jalil, Thomas Pilot, Thibaut Bourgeois, Aline Laubriet, Xiaoxu Li, Marc Diedisheim, Valérie Deckert, Charlène Magnani, Naig Le Guern, Jean-Paul Pais de Barros, et al.ProLong™ Diamond Antifade Mountant with DAPI,Thermo-Fisher,Cat# P36961 Mouse CD68 antibody | FA-11,Bio-Rad,Cat#MCA1957T; RRID:AB_2074849 Mouse FcR blocking reagent,Miltenyi,Cat#130-092-575;RRID:AB_2892833 Mouse CD115-PE,Miltenyi,Cat#130-112-639;RRID:AB_2654553 Mouse

(16) Long-term systemic androgen deprivation partially modulates neuroinflammation in male AppNL-G-F/NL-G-F miceSci RepApril 27, 2025Kasumi Maekawa, Akira Sobue, Okiru Komine, Yuko Saito, Shigeo Murayama, Takaomi C. Saido, Takashi Saito, Koji YamanakaJapan), mouse anti-human amyloid β (N) (82E1) (#10326, 1:200, IBL, Japan), rat anti-CD68 (FA-11) (#MCA1957T, 1:1000, Bio-Rad, USA) and rabbit anti-BACE1 (D10E5) (#5606, 1:100, Cell Signaling Technology, Danvers,

(17) Macrophage Deficiency of Sorting Nexin 25 Suppress Plaque Formation in Atherosclerotic Lesions in MiceACTA HISTOCHEMICA ET CYTOCHEMICAApril 26, 2025Ayami Isonishi, Kouko Tatsumi, Hiroaki Okuda, Tatsuhide Tanaka, Tsuyoshi Hattori, Akio Wanakafollowing antibodies: rat anti-CD68 (1:2000 for enzyme antibody method, 1:200 for immunofluorescence, MCA1957T, Bio-Rad, Hercules, CA, USA) and rabbit anti-SNX25 (1:200, 13294-1-AP, Proteintech Group, Chicago, IL,

(18) Sex differences in the microglial response to stress and chronic alcohol exposure in miceBiol Sex DifferMarch 4, 2025Alexa R. Soares, Vernon Garcia-Rivas, Caroline Fai, Merrilee Thomas, Xiaoying Zheng, Marina R. Picciotto, Yann S. Mineurdonkey anti-goat Alexa 488 (Invitrogen A-11055) Morphology/Lysosome,[1:1000] rat anti-CD68 (BioRad MCA1957T),[1:1000] donkey anti-rat Alexa 555 (Abcam ab150154) Polarization,[1:1000] rabbit anti-iNos (Abcam

(19) Nicotinamide mononucleotide restores impaired metabolism, endothelial cell proliferation and angiogenesis in old sedentary male miceiScienceJanuary 17, 2025Kevin Kiesworo, Thomas Agius, Michael R. Macarthur, Martine Lambelet, Arnaud Lyon, Jing Zhang, Guillermo Turiel, Zheng Fan, Ph.D. Sènan d’Almeida, Korkut Uygun, et al.Pharmingen,Cat#555289 Rat Anti-Mouse CD45,BD Biosciences,Cat#550539 Rat Anti Mouse CD68,Biorad,Cat#MCA1957T Rabbit anti-HO-1,Abcam,Cat#Ab13243 Rabbit anti-ERG,Cell Signaling Technology,Cat#Ab92513 Mouse Anti-

(20) Expression of Lumican and Osteopontin in Perivascular Areas of the Glioblastoma Peritumoral Niche and Its Value for PrognosisInternational journal of molecular sciencesDecember 29, 2024Salinas MD, Rodriguez P, Rubio G, Valdor R.MA, USA), goat anti-Iba-1 (1/1000; ab5076, Abcam, Amsterdam, The Netherlands), rat anti-CD68 (1/500; MCA1957T, Biorad, Hercules, CA, USA), rabbit anti-Lumican (1/200; bs-5890R, Bioss, Woburn, MA, USA) and rabbit

Write a Review

(1)

1/7/2016Mouse Skin CD68 Lin ChenOur goal is to examine macrophages in mouse skin using IF. We tried other macrophage antibodies such Moma 2 and F4/80, this clone (FA-11) worked much better than others
(1)

7/1/2015Excellent recognition of CD68+ macrophages in various tissues of the mice Rahul KeswaniMacrophages can be identified in tissue cross-sections via the CD68 intracellular marker. We used this product along with the use of Cell Signaling Tech's AlexaFluor488 (Catalog No. 4416S) secondary ... Read Review

RAT ANTI MOUSE CD68

RAT ANTI MOUSE CD68

RAT ANTI MOUSE CD68

RAT ANTI MOUSE CD68

RAT ANTI MOUSE CD68 from Bio-Rad (Formerly AbD Serotec)

Questions and Answers

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RAT ANTI MOUSE CD68 from Bio-Rad (Formerly AbD Serotec)

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