Rabbit anti-MAD2L2 was used at 1:500to detect human MAD2L2 by WB. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa).
Affinity Purified anti-MAD2L2 antibody shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, Uppsala, Sweden.
Supplier Page from OriGene Technologies for MAD2L2 Rabbit Polyclonal Antibody