WB using anti-NRF1 antibody shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at RT for 1 h followed by detection using IRDye‚Ñ¢700 labeled Goat-a-Rabbit IgG [H&L] (611-130-122) diluted 1:2, 500.