WB using anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recom-binant human IL-32A (lane 1).After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000.