HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PRKCZ (Cat# RC202472, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCZ(Cat# TA590141). Positive lysates [LY400967] (100ug) and [LC400967] (20ug) can be purchased separately from OriGene.