ERp57 (GRP58) Antibody: RPE

Immunohistochemistry analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (SMC-168). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (SMC-168) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis and Hair Follicles.

Western Blot analysis of Human cell lysates showing detection of Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (SMC-168). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (SMC-168) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (SMC-168). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (SMC-168) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining.