Slo2.2 Antibody: RPE

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 (SMC-323). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (SMC-323) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.

Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.2 Potassium Channel protein using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 (SMC-323). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (SMC-323) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 (SMC-323). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (SMC-323) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Suprabasal epidermal staining. Hair follicles negative.