CEACAM8/CD66b Antibody (G10F5)

Product Specs
ItemCEACAM8/CD66b Antibody (G10F5) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $359.00Supplier Page View Company Product Page
Catalog NumberNB100-77808
Quantity0.1 mg (also 0.025 mg)
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready,Immunocytochemistry/ Immunofluorescence
Concentration1.0 mg/ml
FormatPBS
Conjugate/TagUnconjugated
PurityIgM purified
TargetCEACAM8/CD66b
ReactivityHuman,Mouse
IsotypeIgM Kappa
HostMouse
CloneG10F5
Antibody TypePrimary Antibodies
ImmunogenHuman peripheral blood cells
ClonalityMonoclonal
Research AreaCancer
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene NameCEA cell adhesion molecule 8
NCBI Gene AliasesCD66b, CD67, NCA-95, CGM6

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunofluorescence staining in my research on neutrophils?

Yes, the CEACAM8/CD66b Antibody (G10F5) has been successfully utilized in immunofluorescence staining, specifically at a dilution of 1:100, to evaluate the colocalization of CD66b with other white blood cell markers, including T-lymphocytes and macrophages, as documented in scientific literature ().

Is this antibody effective for flow cytometry applications?

The CEACAM8/CD66b Antibody (G10F5) is listed among the applications for flow cytometry, indicating its suitability for this technique. It has been cited in studies that involve the analysis of immune cell populations, which suggests its effectiveness in such applications.

What species does this antibody react with, and is it suitable for human studies?

This antibody is reactive with both human (Homo sapiens) and mouse (Mus musculus) species. Its application in human studies is supported by its use in research involving human peripheral blood cells, making it appropriate for studies focused on human biology.

What is the recommended storage condition for this antibody to maintain its stability?

The CEACAM8/CD66b Antibody (G10F5) should be stored at 4°C for short-term use and aliquoted for long-term storage at -20°C. It is important to avoid freeze-thaw cycles to preserve the integrity of the antibody.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary diseaseNature CommunicationsMarch 11, 2026Shi-Xia Liao, Yan-Wen Wang, Ling-Mei Shi, Lan-Ying Zhang, Jian Zhou, Peng-Peng Sun, Huai-Yu Hu, Yu-Ting Liu, Xuan An, Jing-Qing Xu, et al.Ly-6G (Invitrogen, 14-5931-82, 1:50), αSMA (Invitrogen, 50976082, 1:200), CD66b (Novus Biologicals, NB100-77808, 1:100), SFTPC (Proteintech, 10774-1-AP, 1:200), MPO (Proteintech, 22225-1-AP, 1:200), NE (Abclonal,

(2) C-reactive protein expression in acute ischemic stroke blood clots: Implications for etiologyEuropean stroke journalSeptember 1, 2025Liu W, Sahin C, Güner Sak N, Giraud A, Messina P, Bozsak F, Darcourt J, Sacchetti F, Januel AC, Bellanger G, et al.Abcam), anti-Fibrinogen (1:1000, ab58207, Abcam), anti-CD66b (1:100, ab955, Abcam), anti-CD68 (1:2000, NB100-77808, Novus Biologicals), anti-CD3 (1:20, ab17143, Abcam), and anti-glycophorin A (1:100, ab212432, Abcam).

(3) Single cell and spatial transcriptomics highlight the interaction of club-like cells with immunosuppressive myeloid cells in prostate cancerNature communicationsNovember 16, 2024Kiviaho A, Eerola SK, Kallio HML, Andersen MK, Hoikka M, Tiihonen AM, Salonen I, Spotbeen X, Giesen A, Parker CTA, et al.(AE1/AE3/PCK26) (Roche, 760-2135), anti-CP 1:150 (HPA001834, Sigma-Aldrich), anti-CD66b 1:50 (Novus Biologicals, NB100-77808), anti-CXCR2 1:2000 (Abcam, ab245982), anti-CD45 1:100 (Cell Signaling Technology, #13917) and anti-CD11b

(4) Electrical stimulation of the vagus nerve ameliorates inflammation and disease activity in a rat EAE model of multiple sclerosisProceedings of the National Academy of Sciences of the United States of AmericaJuly 9, 2024Natarajan C, Le LHD, Gunasekaran M, Tracey KJ, Chernoff D, Levine YA.conjugated rabbit anti-CD31 (Cat #Ab218582, Abcam, Waltham, MA); and mouse anti-CEACAM8/CD66b (Cat #NB100-77808, Novus Biological LLC, Centennial, CO) at 1:100 concentration in 1% normal goat serum at 4 °C overnight.

(5) Investigating the Role of Brain Natriuretic Peptide (BNP) and N-Terminal-proBNP in Thrombosis and Acute Ischemic Stroke EtiologyInt J Mol SciMarch 5, 2024Rosanna Rossi, Duaa Jabrah, Andrew Douglas, James Prendergast, Abhay Pandit, Michael Gilvarry, Ray McCarthy, Petra Redfors, Annika Nordanstig, Turgut Tatlisumak, et al.(Abcam, Cambridge, UK, ab955, 1:50); and mouse anti-CD66b (Novus Biologicals, Centennial, CO, USA, NB100-77808, 1:100). Alexa Fluor 594-labelled goat anti-mouse IgG H&L (Abcam Cambridge, UK, ab150116, 1:200) and

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(7) LncRNA H19 aggravates primary graft dysfunction after lung transplantation via KLF5-mediated activation of CCL28American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsOctober 1, 2023Li J, Han Z, Zhu Z, Wei L.

(8) Macrophage-endothelial cell crosstalk orchestrates neutrophil recruitment in inflamed mucosaThe Journal of clinical investigationAugust 1, 2023Ren X, Manzanares LD, Piccolo EB, Urbanczyk JM, Sullivan DP, Yalom LK, Bui TM, Lantz C, Najem H, Dulai PS, et al.

(9) Spatially Resolved Transcriptomics Deconvolutes Prognostic Histological Subgroups in Patients with Colorectal Cancer and Synchronous Liver MetastasesCancer researchApril 14, 2023Wood CS, Pennel KAF, Leslie H, Legrini A, Cameron AJ, Melissourgou-Syka L, Quinn JA, van Wyk HC, Hay J, Roseweir AK, et al.

(10) Role of immune-inflamed phenotype in the prognosis of hypopharyngeal carcinoma following primary surgeryHead & neckJanuary 1, 2023Chen R, Cai Q, Lin P, Liang F, Han P, Zhang L, Song P, Zhang T, Huang X.

(11) Estradiol suppresses psoriatic inflammation in mice by regulating neutrophil and macrophage functionsThe Journal of allergy and clinical immunologyOctober 1, 2022Adachi A, Honda T, Egawa G, Kanameishi S, Takimoto R, Miyake T, Hossain MR, Komine M, Ohtsuki M, Gunzer M, et al.

(12) PET Imaging of System x(C) (-) in Immune Cells for Assessment of Disease Activity in Mice and Patients with Inflammatory Bowel DiseaseJournal of nuclear medicine : official publication, Society of Nuclear MedicineOctober 1, 2022Seo M, Kim Y, Ye BD, Park SH, Kim SY, Jung JH, Hwang SW, Chae SY, Lee DY, Lee SJ, et al.

(13) S100b in acute ischemic stroke clots is a biomarker for post-thrombectomy intracranial hemorrhagesFrontiers in neurologyJanuary 1, 2022Rossi R, Douglas A, Gil SM, Jabrah D, Pandit A, Gilvarry M, McCarthy R, Prendergast J, Jood K, Redfors P, et al.anti-CD3 (abcam ab17143, 1:10); mouse anti-CD68 (abcam ab955, 1:50) mouse anti-CD66b (Novus biological NB100-77808, 1:100). Secondary antibodies were: Goat anti-mouse IgG H&L (Alexa Fluor 594, abcam ab150116, 1:200)

(14) Sex-Dependent Hepatoprotective Role of IL-22 Receptor Signaling in Non-Alcoholic Fatty Liver Disease-Related FibrosisCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Abdelnabi MN, Flores Molina M, Soucy G, Quoc-Huy Trinh V, Bédard N, Mazouz S, Jouvet N, Dion J, Tran S, Bilodeau M, et al.

(15) Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear poreGenes & developmentDecember 1, 2020Pinzaru AM, Kareh M, Lamm N, Lazzerini-Denchi E, Cesare AJ, Sfeir A.

(16) Extracellular-Regulated Protein Kinase 5-Mediated Control of p21 Expression Promotes Macrophage Proliferation Associated with Tumor Growth and MetastasisCancer researchAugust 15, 2020Giurisato E, Lonardi S, Telfer B, Lussoso S, Risa-Ebrí B, Zhang J, Russo I, Wang J, Santucci A, Finegan KG, et al.

(17) ITLN1 modulates invasive potential and metabolic reprogramming of ovarian cancer cells in omental microenvironmentNature communicationsJuly 15, 2020Au-Yeung CL, Yeung TL, Achreja A, Zhao H, Yip KP, Kwan SY, Onstad M, Sheng J, Zhu Y, Baluya DL, et al.Thermo Fisher Scientific), anti-CD11b (1:150, AC-0043RUO, Epitomics Inc., Burlingame, CA, USA), and anti-CD66b (1:50, NB100-77808, Novus Biologicals) at the room temperature for 1 h. Next, incubation with Opal Polymer HRP Ms + Rb

(18) Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cellsActa neuropathologica communicationsFebruary 3, 2020Rodríguez-Lorenzo S, Konings J, van der Pol S, Kamermans A, Amor S, van Horssen J, Witte ME, Kooij G, de Vries HE.

(19) Role of natural killer cells in the pathogenesis of human acute graft-versus-host diseaseTransplantationJuly 1, 1993Rhoades JL, Cibull ML, Thompson JS, Henslee-Downey PJ, Jennings CD, Sinn HP, Brown SA, Eichhorn TR, Cave ML, Jezek DA.

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