Western Blot: UTP18 Antibody [NB100-77294] - Detection of Human UTP18/WDR50 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UTP18/WDR50 antibody NB100-77294 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. UTP18/WDR50 was also immunoprecipitated by rabbit anti-UTP18/WDR50 antibody NB100-77295, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).