Fig.1) Western blot analysis was performed on nuclear enriched extracts (30 µglysate) of MCF7 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), Jurkat (Lane 4) and HEK-293 (Lane 5). Fig2.) Likewise, western blot analysis was performed on tissue extracts (30 µglysate) of Mouse Liver, Mouse Spleen and Mouse Lung. The blots were probed with LXR alpha/beta Rabbit polyclonal Antibody (Product # PA1-331B, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 40 kDa band corresponding to LXR beta was observed across the cell lines tested. Apart from this in Fig. 2, 50 kDa corresponding to LXR alpha form is also observed across all tissue extracts tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).