Fig 2: Elevation of retinal tissue induction of visual cycle enzymes (A), and retinoid binding proteins (B), and stimulated by retinoic acid 6 (STRA6, C) in chrysin-treated mice. The db/db mice were orally supplemented with 10 mg/kg of chrysin daily for 10 weeks. The db/m mice were introduced as control animals. Mouse retinal tissue extracts were subject to Western blot analysis with a primary antibody against each target protein of lecithin-retinol acyltransferase (LRAT), retinol dehydrogenase 5 (RDH5), cellular retinol binding protein (CRBP), cellular retinaldehyde-binding protein (CRALBP), interphotoreceptor retinoid-binding protein (IRBP), or STRA6. ß-actin protein was used as an internal control. Bar graphs (mean ± SEM, n = nine independent experiments) in the bottom or right panels represent densitometric results of upper blot bands. * Values in bar graphs indicate a significant difference at p < 0.05.