Fig 2: ZQFZ regulated immune-related proteins of spleen and colorectum in ApcMin/+ mice. Base on the result of immunohistochemical staining, ZQFZ enhanced (A) CD4 and (B) CD8, no affect (C) IL-4, and enhanced (D) IFN-? in spleen, and enhanced (E) CD4 and (F) CD8, no affect (G) IL-4, and enhanced (H) IFN-? in colorectal tumors (50×, scale bar: 200 µm; 400×, scale bar: 20 µm). The protein expression levels of (I) CD4, CD8, CTLA4 and PD-L1 in colorectal tumor were detected by western blotting. The quantitative data of the protein expression levels were normalized by GAPDH expressions and were shown as a percentage of the corresponding relative intensity of the CTRL group. Data are shown as the mean ± SD and analyzed via a one-way ANOVA test. (n = 3). **p < 0.01 and ***p < 0.001 vs. CTRL group.