NLRP3 Polyclonal Antibody bs-10021R from Bioss Inc.

Product Specs
ItemNLRP3 Polyclonal Antibody
CompanyBioss Inc.
Price $375.00Supplier Page View Company Product Page
Catalog Numberbs-10021R
Format0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
Tissuebrain, eye, spinal cord
ImmunogenKLH conjugated peptide, human Cryopyrin
ReactivityHu, Ms, Rt, Dg
ClonalityPolyclonal
NCBI Gene AliasesMWS, AGTAVPRL, CLR1.1, CIAS1, FCU, FCAS, C1orf7, AVP, KEFH, DFNA34, NALP3, AII, FCAS1, PYPAF1
NCBI Full Gene NameNLR family pyrin domain containing 3
ApplicationsWB, ELISA, FCM, ICC, IF, IHC, IHC-fr, IHC-p
Antibody TypePrimary Antibody
HostRabbit
IsotypeIgG
TargetCIAS1
Conjugate/TagUnconjugated
Concentration1ug/ul
Quantity100ul, 20uL

Questions and Answers

Can this antibody be used to study the role of NLRP3 in neuroinflammation related to Alzheimer's disease?

Yes, the NLRP3 Polyclonal Antibody has been utilized in studies investigating neuroinflammation in Alzheimer's disease. For instance, Ebrahimi et al. (2018) demonstrated that NLRP3 is upregulated in murine astrocytes stimulated with amyloid β1–42, which is a key component in the pathogenesis of Alzheimer's disease. The antibody was used to assess NLRP3 expression levels in this context.

Is this antibody effective for detecting NLRP3 in immunohistochemistry applications?

Yes, the NLRP3 Polyclonal Antibody has been shown to be effective in immunohistochemistry applications. It has been used in various studies, including Kariya et al. (2020), where it was applied to assess NLRP3 expression in inflammatory cells in a model of otitis media, indicating its utility in visualizing NLRP3 in tissue samples.

What species can this antibody be used with for research purposes?

The NLRP3 Polyclonal Antibody is reactive with several species, including human, mouse, rat, and canine. This broad reactivity allows researchers to utilize it in various experimental models across different species.

Has this antibody been cited in studies related to the NLRP3 inflammasome and its role in inflammation?

Yes, the NLRP3 Polyclonal Antibody has been cited in multiple studies focusing on the NLRP3 inflammasome's role in inflammation. For example, Kang et al. (2022) used this antibody to investigate the effects of ginsenoside Rg1 on NLRP3 inflammasome activation in porcine intestinal cells, demonstrating its relevance in studies of inflammatory pathways.

Bioss Inc.

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Woburn, MA 01801

United States

Phone: 800.501.7654 (Toll free)
Phone: +1.781.569.5821 (International)

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Company Profile

Website: www.biossusa.com

(1) Thymoquinone Attenuates Aluminum Chloride-Induced Testicular Injury by Inhibiting NLRP3/Caspase 1/IL-1β Inflammasome Signaling and Polarizing the Macrophages Toward Anti-Inflammatory M2 PhenotypeCellsDecember 1, 2025Heba M. Elhessy, Basma Adel Khattab, Alaa M. Badawy, Hassan Reda Hassan Elsayed, Mansour A. Alghamdi, Hind Zafrah, Mohammed R. Rabei, Ola A. Habotta, Nermeen H. Lashinerabbit polyclonal antibody for NLRP3, caspase-1, IL-1β, CD163 and monoclonal antibody for CD68 (Bioss; bs-10021R, ServiceBio; GB11383, ServiceBio; GB11113, Cat; ER2001-15, and Genemed; 61-0184 respectively) (1:200,

(2) HHQG ameliorates acute liver injury (ALI) by inhibiting NLRP3 activation through RASD1-mediated regulation of the PKCδ-NF-κB signaling pathwayScientific ReportsNovember 18, 2025Chunli Ma, Linghong Wang, Lili Cao, Linyun Zhao, Xin Zhang, Chengyou Ma, Hua Lian, Xiufang Bao, Mutu Eerde, Qiqige Buren, et al.G1340), hematoxylin solution (BASO, BA4097), eosin solution (BASO, BA4098), anti-NLRP3 antibody (Bioss, bs-10021r), anti-GSDMD antibody (Abcam, ab219800), anti-RASD1 antibody (Santa Cruz, sc-398988), anti-RASD1 antibody

(3) Protective Effect of Neochlorogenic Acid on Diabetic Nephropathy via Inflammation and Pyroptosis SuppressionJ Agric Food ChemOctober 22, 2025I-Ning Tsai, Yung-Che Tsai, Hui-Pei Huang, Ching-Chun Chen, Tung-Wei Hung, Chau-Jong WangICAM-1 (Santa Cruz, sc-107), COX-2 (Santa Cruz, sc-19999), iNOS (Santa Cruz, sc-7271), NLRP3 (Bioss, bs-10021R), GSDMD (Invitrogen, PA5-104324), IL-1β (abcam, ab283818), and Caspase-1 p20 (Bioss, bs-10442R). 2.4

(4) Intrathecal STAT3 inhibitor Bt354 ameliorates chronic constriction injury-induced nociceptive sensitization by modulating neuroinflammationNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsOctober 1, 2025Cheng HJ, Chen NF, Tang YC, Shih PC, Chen WF, Chiu YJ, Sung CS, Wen ZH.rabbit monoclonal anti-pTBK1 (5483, cell signaling, Danvers, MA, USA), rabbit polyclonal anti-NLRP3 (bs-10021R, Bioss, Woburn, MA, USA), mouse monoclonal anti-STAT3 (9139s, cell signaling), rabbit monoclonal anti-pSTAT3

(5) JC124 confers multimodal neuroprotection in epilepsy by suppressing NLRP3 inflammasome activation: evidence from animal and human neuronal modelsCell communication and signaling : CCSJuly 8, 2025Zhang P, Li Z, Liang M, Yuan J, Zhou J, Zhang J, Deng J, Chen Y, Tang H, Xu C, et al.serum blocking, the paraffin slices were treated with the following antibodies: anti-NLRP3 (1:150, bs-10021R, BIOSS, Beijing, China), anti-ASC (1:200, #67824, CST), anti-Caspase-1 p10 (1:150, AF4022, Affinity),

(6) Polystyrene nanoplastics trigger pyroptosis in dopaminergic neurons through TSC2/TFEB-mediated disruption of autophagosome-lysosome fusion in Parkinson’s diseaseJ Transl MedJune 5, 2025Xiaomei Liang, Yaqi Zeng, Piao Zhang, Baoyu Zhu, Jiezhu Feng, Tongtong Deng, Zhongling Fu, Chengshuai Liu, Chengyu Chen, Yuhu Zhangovernight with primary antibodies: anti-tyrosine hydroxylase (TH) (ab137869, Abcam, UK), anti-NLRP3 (bs10021R, BIOSS, China), anti-lysosomal-associated membrane protein 1 (LAMP1) (ab278043, Abcam, UK), anti-P62

(7) Impact of intermittent fasting versus vitamin D on high fat fructose-induced pancreatic steatosis: possible role of aquaporinsMol MedMay 26, 2025Basma Adel Khattab, Maha Osman Hammad, Zienab Helmy Eldken, Doaa Hellal, Sherin Zohdy Mohamed, Noha Hammad Sakrantibody (bs-2506R, Bioss, Woburn, USA, at 1:200 dilution) and primary rabbit polyclonal NLRP3 antibody (bs-10021R, Bioss, Woburn, USA, at 1:200 dilution) at 4◦C overnight. The next day, phosphate-buffered saline was

(8) A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse modelSci ProgApril 23, 2025Ji Luo, Zheng Lei, Hongyu Zheng, Rudan Zhouwith primary antibodies at 4 °C overnight. The primary antibodies used were as follows: NLRP3 (Bioss, bs-10021R, 1:2000), IL-6 (Bioss, bs-4587R, 1:1000), IL-1beta (Bioss, bs-0812R, 1:1000), and ICAM-1 (Bioss, bs-0608R,

(9) Denfivontinib Activates Effector T Cells Through the NLRP3 Inflammasome, Yielding Potent Anticancer Effects by Combination with PembrolizumabMolecular Cancer TherapeuticsMarch 4, 2025Dong Kwon Kim, Chun-Bong Synn, Wongeun Lee, Ha-Ni Jo, Chai Young Lee, Seul Lee, Joon Yeon Hwang, Youngtaek Kim, Seong-san Kang, Sujeong Baek, et al.hour at room temperature. Subsequently, cells were incubated with primary antibodies against NLRP3 (#bs-10021R, 1:400, Bioss) and ASC-conjugated with Alexa Fluor 594 (#sc-365202, 1:400, Santa cruz) for 1 hour at

(10) NLRP3-inflammasome Related Genes as Emerging Biomarkers and Therapeutic Targets in PsoriasisInflammationMarch 3, 2025Ao Shi, Yuan Shu, Kaibo Hu, Shivon Sudesh, Ying TuBeijing Zhongshan) at 37 ℃ for 1 h. Then the samples were incubated with primary antibody (1:100, #bs-10021r, #bs-6741r, #bs-3897r, #bs-10442r, BIOSs) overnight at 4 °C. After this, samples were rinsed with 0.01

(11) Cardamom extract alleviates tamoxifen-induced liver damage by suppressing inflammation and pyroptosis pathwaySci RepFebruary 8, 2025Wedad S. Sarawi, Hala A. Attia, Afraa Alzoubi, Nour Alanazi, Raeesa Mohammad, Rehab A. Aliinterleukin-6 (IL-6, SEKR-0005) were purchased from Solarbio, China. Primary antibodies were NLRP3 (bs-10021R, Bioss, Massachusetts, USA), caspase-1(K107599P, Solarbio, China), GSDMD, (K011353P, Solarbio, China),

(12) tVNS alters inflammatory response in adult VPA‐induced mouse model of autism: evidence for sexual dimorphismFEBS Open BioOctober 14, 2024Hale Gök Dağıdır, Neslihan Bukan, Meltem Bahcelioglu, Ayşen Çalıkuşu, Ece Alim, Saadet Özen Dizakar, Elif Topa, Hayrunnisa Bolaya blocking solution (TP‐125‐UB, Thermo Scientific) and incubated with rabbit anti‐NLRP3 (1 : 1000, bs‐10021R, Bioss, China) primary antibody overnight at 4 °C. Afterwards, a secondary antibody (anti‐rabbit IgG

(13) Long Non-coding RNA NEAT1 , NOD-Like Receptor Family Protein 3 Inflammasome, and Acute Kidney InjuryJournal of the American Society of NephrologyAugust 1, 2024Rui Xue, Wai Han Yiu, Kam Wa Chan, Sarah W.Y. Lok, Yixin Zou, Jingyuan Ma, Hongyu Li, Loretta Y.Y. Chan, Xiao Ru Huang, Kar Neng Lai, et al.pro-caspase-1, and cleaved caspase-1 (AG-20B-0044, AdipoGene Life Sciences, San Diego, CA); and NLRP3 (bs-10021R, Bioss Antibodies, Woburn, MA) were used. ELISA The human serum level of IL-6 (D6050B) and IL-8 (DY208)

(14) Astragaloside IV alleviates renal fibrosis by inhibiting renal tubular epithelial cell pyroptosis induced by urotensin II through regulating the cAMP/PKA signaling pathwayPLOS ONEMay 31, 2024Lin Zhang, Wenyuan Liu, Sufen Li, Jinjing Wang, Dalin Sun, Hui Li, Ziyuan Zhang, Yaling Hu, Jingai Fangsolution. Added 5% BSA (1:200) diluted primary antibody (IL-1β antibody, bs-0812R; NLRP3 antibody, bs-10021R; and Caspase-1 antibody, bs-0169R; Beijing Bioss Company. GSDMD-N antibody, ER1901-37, Zhejiang Hua

(15) The SK4 channel allosteric blocker, BA6b9, reduces atrial fibrillation substrate in rats with reduced ejection fractionPNAS NexusMay 12, 2024Shira Burg, Or Levi, Sigal Elyagon, Shir Shapiro, Michael Murninkas, Sharon Etzion, Gideon Gradwohl, Daria Makarovsky, Alexandra Lichtenstein, Yaara Gordon, et al.ab124964, abcam, rabbit monoclonal antibody against the N-terminus of human α-SMA, 1:1,000) and NLRP3 (bs-10021R, Bioss Antibodies, rabbit polyclonal antibody against human NLRP3, 1:200), respectively, for 40 min,

(16) High-Dose Fenofibrate Stimulates Multiple Cellular Stress Pathways in the Kidney of Old RatsInternational journal of molecular sciencesMarch 6, 2024Wrońska A, Kieżun J, Kmieć Z.duet (#8208S; Cell Signaling Technology, Leiden, The Netherlands), IL-6 (#bs-4539R, Bioss), Nlrp3 (#bs-10021R, Bioss), Nrf2 (#bs-1074R, Bioss), Pgc1α (#20658-1-AP, Proteintech, Rosemont, IL, USA), Pparα (#AF5301,

(17) Platycodon D protects human nasal epithelial cells from pyroptosis through the Nrf2/HO-1/ROS signaling cascade in chronic rhinosinusitisChinese medicineMarch 4, 2024Wang R, Wang Y, Liu H, Zhu J, Fang C, Xu W, Lu Z, Yan Y, He W, Ruan Y, et al.were incubated with primary antibodies (Rabbit Anti-GSDMD-NT, AF4012, Affinity; Rabbit Anti-NLRP3, bs-10021R, Bioss; Rabbit Anti-cleaved Caspase-1, AF5418, Affinity; dilution: 1:200), then incubated with secondary

(18) Suppression of NLRP3 inflammasome activation by astragaloside IV via promotion of mitophagy to ameliorate radiation-induced renal injury in miceTranslational andrology and urologyJanuary 31, 2024Ding Y, Liu S, Zhang M, Su M, Shao B.Japan). Immunohistochemistry Immunohistochemical staining was performed as follows: sections were incubated with NLRP3 (bs-10021R; Bioss Antibodies, Woburn, MA, USA), caspase-3 (bs-20364R; Bioss Antibodies), and LC3 (14600-1-AP, Proteintech,

(19) Vaporization of perfluorocarbon attenuates sea-water-drowning-induced acute lung injury by deactivating the NLRP3 inflammasomes in caninesExperimental biology and medicine (Maywood, N.J.)January 1, 2024Su CC, Zhang ZR, Liu JX, Meng JG, Ma XQ, Mo ZF, Ren JB, Liang ZX, Yang Z, Li CS, et al.polyclonal primary antibodies for NOD-like receptor family pyrin domain containing 3 (NLRP3) (1:500; bs-10021R, Bioss, Beijing, China), nuclear respiratory factor-1 (NRF1) polyclonal antibodies (1:1000; MBS3204137,

(20) Irisin Ameliorated Skeletal Muscle Atrophy by Inhibiting Fatty Acid Oxidation and Pyroptosis Induced by Palmitic Acid in Chronic Kidney DiseaseKidney and Blood Pressure ResearchSeptember 15, 2023Ting Zhou, Shiyuan Wang, Yajing Pan, Xingtong Dong, Leiyun Wu, Jiali Meng, Jialing Zhang, Qi Pang, Aihua Zhangselected to calculate the integral optical density of positive staining. Antibodies against NLRP3 (bs-10021R; Bioss, Beijing, China), Caspase-1 (22915-1-AP; Proteintech, Wuhan, China), and CPT1A (ab234111; Abcam,

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NLRP3 Polyclonal Antibody from Bioss Inc.

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