Knockout validation by Western blot analysis of Caspase 1 in Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. Samples were incubated in Caspase 1 polyclonal antibody (Product # PA1-41214) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.