Fig 1: Characterization of Lin- lymphoid cells among human PBMCs A CD127+ and CD127- cells from Lin-CD56- population after gating on lymphoid, singlet, live, CD45+ cells of PBMCs. Lineage (Lin) markers include antibodies against: CD3, CD4, TCRaß, TCR?d, CD19, CD20, CD22, CD34, FceRIa, CD11c, CD303, CD123, CD1a, and CD14.B Heatmap of differentially expressed genes by RNA-Seq, sorted Lin-CD56-CD16-CD127+ versus Lin-CD56-CD16-CD127- cells, from PBMCs of five donors (log2 fold change > 1, Padj < 0.01 determined by DESeq2, the postsort profiles are shown in Appendix Fig S1).C Normalized counts of Lin-CD56-CD127+ (red) and Lin-CD56-CD127- (blue) cell-related genes from (B) by DESeq2 (n = 5).D, E Reactome analysis based on enriched transcripts of Lin-CD56-CD127+ cells (D) or Lin-CD56-CD127- cells (E).F Gating of Lin-CD56hi NK, Lin-CD56dim NK, Lin-CD56- NK cells, and Lin-CD56-CD16-CD127+ILCs.G The indicated populations in (F) were detected with isotype controls or antibodies against TBX21, CRTH2, and ROR?T. Data information: All data were generated using blood from healthy HIV-1-negative donors.