Immunofluorescent analysis of alpha internexin in rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained using an alpha internexin polyclonal antibody (Product # PA1-10017) at a dilution of 1:2,000 as seen in green, using a GFAP polyclonal antibody (Production # PA1-10004) at a dilution of 1:5,000 as seen in red, and with DAPI staining the nuclear DNA in blue. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.