MHC Class I Heavy Chain (Restricted expression) Mouse Monoclonal Antibody [Clone ID: HC10] AM33035PU-N from OriGene Technologies

Product Specs
ItemMHC Class I Heavy Chain (Restricted expression) Mouse Monoclonal Antibody [Clone ID: HC10]
CompanyOriGene Technologies
Price $595.00Supplier Page View Company Product Page
Catalog NumberAM33035PU-N
Quantity100 µg
ApplicationsELISA, EM, FC, IF, IHC, IP, WB
Concentrationlot specific
Conjugate/TagUnconjugated
FormatPBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
ReactivityHuman
IsotypeIgG2a
CloneHC10
HostMouse
Antibody TypePrimary Antibodies
ImmunogenDerived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains
TypePrimary Antibodies
ClonalityMonoclonal

Questions and Answers

Can this antibody be used to study MHC Class I antigen presentation in human cells?

Yes, the MHC Class I Heavy Chain mouse monoclonal antibody (clone HC10) has been shown to be effective in applications such as Western blotting and immunohistochemistry, which are commonly used to analyze MHC Class I expression and function in human cells, as evidenced by its use in studies like those by Domnick et al. (2022) and Ren et al. (2022).

Is this antibody suitable for flow cytometry applications?

The MHC Class I Heavy Chain antibody has been utilized in flow cytometry, as indicated in its application list. This method is particularly useful for analyzing the expression levels of MHC Class I on the surface of various cell types, including dendritic cells, as demonstrated in studies like Liang et al. (2021).

What is the specificity of this antibody regarding human MHC Class I molecules?

This antibody is specifically reactive to human MHC Class I heavy chains, which are crucial for antigen presentation in the immune response. Its specificity has been validated in multiple studies, including its use in detecting MHC Class I in various experimental contexts, such as those reported by Venkadak et al. (2024).

How has this antibody been utilized in research related to cancer immunotherapy?

The MHC Class I Heavy Chain antibody has been referenced in studies investigating the role of MHC Class I in tumor immunity, such as the work by Ren et al. (2022), which highlights its importance in understanding the mechanisms of antitumor immunity and the effects of immune checkpoint blockade in cancer therapy.

OriGene Technologies

9620 Medical Center Drive # 200

Rockville, Maryland 20850

United States

Phone: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254

Company Profile

Website: http://www.origene.com

(1) Androgen Receptor Inhibition Increases MHC Class I Expression and Improves Immune Response in Prostate CancerCancer DiscoveryMarch 3, 2025Lisa N. Chesner, Fanny Polesso, Julie N. Graff, Jessica E. Hawley, Alexis K. Smith, Arian Lundberg, Rajdeep Das, Tanushree Shenoy, Martin Sjöström, Faming Zhao, et al.Table S7), as described above, or Western blot analysis using anti-AR (D6F11), anti-MHCI (OriGene, Cat#AM33035PU-N, RRID:AB_3662758), or anti-Lamin B1 (Cell Signaling Technology, Cat# 15068, RRID:AB_2798695) antibodies.

(2) Androgen Receptor Inhibition Increases MHC Class I Expression and Improves Immune Response in Prostate CancerCancer DiscovDecember 5, 2024Lisa N. Chesner, Fanny Polesso, Julie N. Graff, Jessica E. Hawley, Alexis K. Smith, Arian Lundberg, Rajdeep Das, Tanushree Shenoy, Martin Sjöström, Faming Zhao, et al.Table S7), as described above, or Western blot analysis using anti-AR (D6F11), anti-MHCI (OriGene, Cat#AM33035PU-N, RRID:AB_3662758), or anti-Lamin B1 (Cell Signaling Technology, Cat# 15068, RRID:AB_2798695) antibodies.

(3) Lineage-specific canonical and non-canonical activity of EZH2 in advanced prostate cancer subtypesNature communicationsAugust 8, 2024Venkadakrishnan VB, Presser AG, Singh R, Booker MA, Traphagen NA, Weng K, Voss NCE, Mahadevan NR, Mizuno K, Puca L, et al.signaling technology; cat. no. 8457 S or 3700 S; 1:2000 dilution) or MHC Class I (OriGene; cat. no. AM33035PU-N; 1:1000 dilution) or ASCL1 (abcam; cat. no. ab211327; 1:1000 dilution) or HES6 (abcam; cat. no. ab172800;

(4) Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genesNature cell biologyFebruary 1, 2023Sparbier CE, Gillespie A, Gomez J, Kumari N, Motazedian A, Chan KL, Bell CC, Gilan O, Chan YC, Popp S, et al.05-1320; Clone AA19; RRID:AB_1586999), Monoclonal mouse anti-MHC class I heavy chain (Origene, Cat# AM33035PU-N; Clone HC10; RRID: AB_2728622), Monoclonal mouse anti-RFX5 (Santa Cruz Biotechnology, Cat# sc-271756;

(5) Molecular basis of MHC I quality control in the peptide loading complexNature communicationsAugust 10, 2022Domnick A, Winter C, Sušac L, Hennecke L, Hensen M, Zitzmann N, Trowitzsch S, Thomas C, Tampé R.catalogue number ab13518, dilution 1:1000), anti-HLA-A/B/C HC10 (Acris Antibodies, catalogue number AM33035PU-N, dilution 1:1000), anti-HLA-A (Abcam, catalogue number ab52922, dilution 1:1000), anti-ERp57 (Abcam,

(6) Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunityThe Journal of clinical investigationApril 15, 2022Ren J, Li N, Pei S, Lian Y, Li L, Peng Y, Liu Q, Guo J, Wang X, Han Y, et al.antibodies were used: WHSC1/NSD2 (Abcam, ab75359, clone 29D1); anti–human MHC-I heavy chain (Origene, AM33035PU-N, clone HC10); and CD8 (Abcam, ab209775, clone EPR20305). Protein expression was scored and quantified

(7) Proteasome inhibitors restore the STAT1 pathway and enhance the expression of MHC class I on human colon cancer cellsJournal of biomedical scienceNovember 10, 2021Liang YH, Chen KH, Tsai JH, Cheng YM, Lee CC, Kao CH, Chan KY, Chen YT, Hsu WL, Yeh KH.interferon regulatory factor-1 (IRF-1; Cell Signaling Technology, #8478), pan-MHC class I (Origene, AM33035PU-N, Rockville, MD, USA), ß-actin (Abcam, ab8227, Cambridge, UK), GAPDH (Abcam, ab8245), HRP donkey anti-rabbit

(8) Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processingScientific reportsApril 27, 2021Liang YH, Tsai JH, Cheng YM, Chan KY, Hsu WL, Lee CC, Chen KH, Yeh KH.and activators of transcription 1 (pSTAT1, CST, #9167, Danvers, MA, U.S.), pan-MHC class I (Origene, AM33035PU-N, Rockville, MD), transporter of antigen processing 1 (TAP1; Abcam, ab83817, Cambridge, UK), transporter

(9) Alpha-type-1 Polarized Dendritic Cell-based Vaccination in Newly Diagnosed High-grade Glioma: A Phase II Clinical TrialAnticancer researchNovember 1, 2020Mitsuya K, Akiyama Y, Iizuka A, Miyata H, Deguchi S, Hayashi N, Maeda C, Kondou R, Kanematsu A, Watanabe K, et al.

(10) An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in CancerCancer cellOctober 14, 2019Burr ML, Sparbier CE, Chan KL, Chan YC, Kersbergen A, Lam EYN, Azidis-Yates E, Vassiliadis D, Bell CC, Gilan O, et al.,Miltenyi Biotec,Cat#130-103-024;Clone 25-D1.16; RRID: AB_2651952 Monoclonal mouse anti-human MHC class I heavy chain,Origene,Cat# AM33035PU-N;Clone HC10; RRID: AB_2728622 Monoclonal mouse anti-HSP60 (C-10),Santa Cruz,Cat# sc-376240; RRID: AB_10986282 Monoclonal

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MHC Class I Heavy Chain (Restricted expression) Mouse Monoclonal Antibody [Clone ID: HC10] from OriGene Technologies

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MHC Class I Heavy Chain (Restricted expression) Mouse Monoclonal Antibody [Clone ID: HC10] from OriGene Technologies

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