Fig 1: The activation of STAT signaling in CD19+ B cells from AS patients (n = 35) upon stimulation with rIL-3. A The phosphorylation levels of STAT1, STAT3, and STAT4 in CD19+ B cells with 100 ng/ml rIL-35–activated fresh isolated PBMCs were analyzed by flow cytometry. B The mean fluorescence intensity (MFI) of p-STAT1 and p-STAT3 were significantly increased in cells treated with rIL-35 compared with the PBS group. *P < 0.05, **P < 0.01 vs. the PBS group