Fig 1: Expression and subcellular localization of TAS2Rs in human hepatocytes. (A) Gene expression profiling was performed for all 25 human TAS2R genes in Hep3B cells. Expression levels were calculated as relative ratios between TAS2Rs and the internal control gene HPRT1. (B) Representative immunofluorescence (IF) images of Hep3B cells stained for TAS2R31, TAS2R46, TAS2R4, and TAS2R3 receptors. Phalloidin was used to label the actin-rich pseudo-canaliculi formed by Hep3B cells. (C) Representative IF images of human liver sections stained with antibodies against TAS2R3, TAS2R4, TAS2R38, and TAS2R46. BSEP was used as a marker for bile canaliculi in hepatocytes. DAPI was used for nuclear counterstaining. Scale bar: 20 μm.