Fig 2: Western blotting of Rubisco and α-tubulan. Rubisco and α-tubulan in the somatic embryos, plantlets and tuberous roots of cassava cultivar SC8 were detected by Western blotting using antiRubisco-polyclonal antibody from Agrisera (AS07218) and anti-α-tubulin-monoclonal antibody from Sigma-Aldrich (T9026). ARs, adventitious roots.

Fig 3: Western blotting of Rubisco, APX and PrxQ.The expression of Rubisco, APX and PrxQ in leaves of cassava NZ199 diploid (a) and autotetraploid (b) genotypes were detected by western blotting using antiRubisco-polyclonal antibody (AS07218), anti-APX antibody (AS08368) and anti-PrxQ antibody (AS05093) from Agrisera, respectively.

Fig 4: Western blotting of Rubisco and peroxiredoxin. The expressions of Rubisco (a) and peroxiredoxin (b) in apical expanded leaves of SC8and Col1046 were detected by Western blotting using anti-Rubisco polyclonal antibody (AS07218) and anti-peroxiredoxin antibody (AS05093) from Agrisera, respectively. Mr, protein marker; lines a and b, the expression of Rubisco and peroxiredoxin of SC8 in 5 °C for 0 and 10 days, respectively; lines c and d, the expression of Rubisco and peroxiredoxin of Co11046 in 5 °C for 0 and 10 days, respectively

Fig 5: Western blot analysis of Trx-m1 and FTR-C in vegetative cells (V), whole cells (W), and heterocysts (H). (A) The purity of heterocysts was checked by immunoblotting using RbcL and NifH antibodies. Proteins extracted from vegetative cells and enriched heterocysts (1 µg) were loaded in each lane. (B) Trx-m1 and FTR-C were detected using each specific antibody. Protein extracts (20 µg) from vegetative cells, whole cells, or heterocysts were loaded per lane.