PKG-1 (C8A4) Rabbit mAb 3248S from Cell Signaling Technology

Product Specs
ItemPKG-1 (C8A4) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3248S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk
TargetPKG1
HostRabbit
NCBI Gene AliasesAAT8, PKG, PKG1, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha
ClonalityMonoclonal
NCBI Full Gene Nameprotein kinase cGMP-dependent 1
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneC8A4
Molecular Weight78

Questions and Answers

What species can I use this antibody on for my experiments?

The PKG-1 (C8A4) Rabbit mAb is reactive with several species, including humans, mice, rats, and non-human primates.

Has this antibody been used in any specific applications in the literature?

Yes, the PKG-1 antibody has been utilized in Western Blot applications, as evidenced by its use in studies investigating nitric oxide signaling pathways and their effects on vascular smooth muscle cells and neuronal cytolysis.

Can this antibody help in understanding the role of PKG in cardiovascular research?

Indeed, the PKG-1 antibody has been cited in studies exploring the nitric oxide/cGMP/PKG signaling pathway, which is crucial for vascular function and has implications in conditions like pulmonary hypertension and diabetic cardiomyopathy.

Are there any notable findings related to PKG signaling that this antibody has helped uncover?

The PKG-1 antibody has been instrumental in research demonstrating that PKG signaling can inhibit TRPM4 channel activity, contributing to vasodilation mechanisms in cerebral arteries, as well as its role in promoting pancreatic β-cell survival and proliferation through AKT/FOXO1 regulation.

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Cyclic guanosine monophosphate-protein kinase G signaling attenuates aortic valve calcification through ULK1-mediated autophagySignal Transduct Target TherMarch 12, 2026Yan Wang, Fei Xu, Chengxiang Song, Xi Wang, Hao Zhou, Tianyi Qu, Hongde Li, Qiang Luo, Wenhua Lei, Yue Yin, et al.incubated with ALP (ABclonal, A0514), RUNX2 (ABclonal, A11753), Sp7 (Abcam, ab209484), PKGI (CST, #3248), ULK1 (Abcam, ab177472), Beclin (CST, #3738), p62 (CST, #5114), LC3A/B (CST, #4108), DYKDDDDK Tag (CST,

(2) Molecular patterns of the NO-sGC-cGMP pathway in progressive and regressive liver fibrosis modelsSci RepJuly 25, 2025Thomas Sorz-Nechay, Ksenia Brusilovskaya, Philipp Königshofer, Benedikt S. Hofer, Oleksandr Petrenko, Benedikt Simbrunner, Vlad Taru, Katharina Bonitz, Henriette Horstmeier, Kerstin Zinober, et al.(sGCa1, #ab85445, Abcam; sGCa2, #MBS9612306, MyBioSource; sGCb1, #160897-1, Cayman Chemical; PKG1, #3248S, Cell Signaling Technology; PDE5, #sc-398747, Santa Cruz Biotechnology) and respective secondary antibodies

(3) Effects of in vivo treatment with Kv7.4 activator, URO-K10, on the impaired relaxation of pulmonary arteries in the monocrotaline-induced pulmonary hypertensive ratsKorean J Physiol PharmacolJuly 1, 2025Seung Beom Oh, Young Keul Jeon, Nari Choi, Hae Young Yoo, Sung Joon KimArbor, MI, USA",160897,"1:1,000" Rabbit anti-PKG-1 (C8A4),"Cell Signaling Technology, Danvers, MA, USA",3248,"1:1,000" Rabbit anti-ROCK1,"Abcam, Cambridge, UK",ab45171,"1:1,000" Rabbit anti-ROCK2,"Cell Signaling Technology

(4) Pharmacological activation of NO-sensitive guanylyl cyclase ameliorates obesity-induced arterial stiffnessVascular PharmacologyJune 1, 2025Enkhjargal Budbazar, Aylin Balmes, Danielle Elliott, Lisette Peres Peres Tintin, Timo Kopp, Susanne Feil, Robert Feil, Tilman E. Schäffer, Francesca Setawith the following primary antibodies: phosphorylated VASP at serine 239 (3114, CST, 56 ng/mL), cGKI (3248, CST, 0.27 μg/mL), sGC isoform β1 (160897, Cayman Chemical, 0.2 μg/mL), pan-actin (AAN01, Cytoskeleton,

(5) MAPK14/p38α shapes the molecular landscape of endometrial cancer and promotes tumorigenic characteristicsCell ReportsJanuary 1, 2025Sayali Joseph, Xingyuan Zhang, Gaith N. Droby, Di Wu, Victoria Bae-Jump, Scott Lyons, Angie Mordant, Allie Mills, Laura Herring, Blake Rushing, et al.,Cell signaling technology,Cat#5599; RRID:AB_10698593 Rabbit monoclonal anti-PKG-1,Cell signaling technology,Cat#3248; RRID:AB_2067450 Rabbit monoclonal anti-p70S6K,Cell signaling technology,Cat#2708; RRID:AB_390722 Rabbit

(6) Cardiac corin and atrial natriuretic peptide regulate liver glycogen metabolism and glucose homeostasisCardiovascular DiabetologyOctober 28, 2024Wenguo Li, Xianrui Zhang, Zibin Zhou, Wenjun Guo, Mengting Wang, Tiantian Zhou, Meng Liu, Qingyu Wu, Ningzheng Dongand western blotting. The primary antibodies used in this study included those against PkgI (1:1000, 3248S, Cell Signaling), Akt (1:1000, 4691, Cell Signaling, Danvers, MA, USA), phospho (p)-Akt (1:1000, 4060,

(7) Deficiencies in corin and atrial natriuretic peptide-mediated signaling impair endochondral ossification in bone developmentCommunications biologyOctober 23, 2024Zhou Z, Mao X, Jiang C, Li W, Zhou T, Liu M, Sun S, Wang M, Dong N, Wu Q, et al.T, Cell Signaling), Gsk3β (1:1000, 12456, Cell Signaling), Osx (1:1000, ab209484, Abcam), PkgI (1:1000, 3248S, Cell Signaling,), PkgII (1:1000, PA5-101156, Invitrogen), β-catenin (1:1000, ab32572, Abcam), Tcf1

(8) Phosphodiesterase Inhibition to Sensitize Non-Small-Cell Lung Cancer to Pemetrexed: A Double-Edged StrategyCancersJuly 6, 2024Ivanina Foureau AV, Foureau DM, McHale CC, Guo F, Farhangfar CJ, Mileham KF.(#3132); Phospho-VASP(Ser157) (#3111T); Phospho-VASP(Ser239) (# 3114); PKA Ca (# 4782); PKG-1(C8A4) (#3248S); Akt (#9272) and Phospho-Akt(Ser473)(D9E) (#4060) and β-actin (#4967). ADCY9(EPR16188) and GUCY1B3(EPR8822)

(9) Polygonatum sibiricum (Huang Jing) polysaccharide reduces diabetic cardiomyopathy through increasing cyclic guanosine monophosphate‐protein kinase G signaling in diabetic miceJournal of Diabetes InvestigationJuly 1, 2024Shengping Lei, Xin Lu, Lei Yan, Tian Liu, Yan Niu, Jun Yutreatment with 1:1,000 polyclonal GRP78 (#3177), CHOP (#5554), PERK (#5683), phospho‐PERK (#3179), PKG (#3248), PDE5 (#SAB2500767) and β‐actin (#3700) overnight at 4°C. The antibodies were purchased from Cell Signaling Technologies

(10) Differential expression of the enzymes regulating myosin light chain phosphorylation are responsible for the slower relaxation of pulmonary artery than mesenteric artery in ratsThe Korean Journal of Physiology & PharmacologyJanuary 1, 2024Seung Beom Oh, Suhan Cho, Hyun Jong Kim, Sung Joon KimArbor, MI, USA",160897,"1:1,000" Rabbit anti-PKG-1 (C8A4),"Cell Signaling Technology, Danvers, MA, USA",3248,"1:1,000" Rabbit anti-ROCK1,"Abcam, Cambridge, UK",ab45171,"1:1,000" Rabbit anti-ROCK2,"Cell Signaling Technology

(11) Inhaled nitric oxide suppresses neuroinflammation in experimental ischemic strokeJ NeuroinflammationDecember 15, 2023Rebecca I. Sienel, Uta Mamrak, Janina Biller, Stefan Roth, Andreas Zellner, Tipparat Parakaw, Rayomand S. Khambata, Arthur Liesz, Christof Haffner, Amrita Ahluwalia, et al.Tween (TBS-T) for 1 h at RT and then incubated with anti-ICAM-1 (ab222736, Abcam 1:1000), anti-PKG-1 (#3248, Cell Signaling, 1:1000), or anti- Guanylate Cyclase β1 subunit (#160897, Caymanchem, 1:200) primary

(12) Hawthorn leaves flavonoids attenuate cardiac remodeling induced by simulated microgravityPharm BiolDecember 1, 2023Liu T, Ma Y, Zhao H, Wang P, Niu Y, Hu Y, Shen X, Zhang M, Yan B, Yu J.primary antibodies against NPRA (1:1000 dilution, ab14536, Abcam, Cambridge, UK), PKG (1:1000 dilution, #3248, Cell Signalling Technology, MA, USA), PDE5A (1:1000 dilution, ab259945, Abcam, Cambridge, UK), NFATc1

(13) Multiomics analyses reveal dynamic bioenergetic pathways and functional remodeling of the heart during intermittent fastingeLifeSeptember 28, 2023Arumugam TV, Alli-Shaik A, Liehn EA, Selvaraji S, Poh L, Rajeev V, Cho Y, Cho Y, Kim J, Kim J, et al.following primary antibodies: p-AMPKα (2535 S, Cell Signaling), AMPKα (2532, Cell Signaling), PKG-1 (3248 S, Cell Signaling), MYPT1 (2634 S, Cell Signaling), PPARγ (7273, Santa Cruz Biotechnology), p-GSK3α/β

(14) FoxO3 normalizes Smad3-induced arterial smooth muscle cell growthFrontiers in PhysiologyAugust 24, 2023Jake T. Francisco, Andrew W. Holt, Michael T. Bullock, Madison D. Williams, Cere E. Poovey, Nathan A. Holland, Jeffrey J. Brault, David A. TulisGAPDH (#2118; Cell Signaling Technology); lamin A/C (#612621; BD Transduction Laboratories); PKG-1 (#3248; Cell Signaling Technology). The following day, membranes were washed three times for 10 min each and

(15) Intra-amniotic sildenafil treatment improves lung blood flow and pulmonary hypertension in congenital diaphragmatic hernia ratsFrontiers in Bioengineering and BiotechnologyJuly 20, 2023Shiho Yoshida, Alexander M. Kreger, George K. Gitteselectroblotting. Immunodetection was performed with a rabbit anti-protein kinase G (PKG) −1 monoclonal antibody (#3248, Cell Signaling Technology) diluted 1:1000 and a rabbit monoclonal to vascular endothelial growth factor

(16) Downexpression of miR-200c-3p Contributes to Achalasia Disease by Targeting the PRKG1 GeneInternational Journal of Molecular SciencesDecember 30, 2022Lucia Micale, Carmela Fusco, Grazia Nardella, Orazio Palmieri, Tiziana Latiano, Domenica Gioffreda, Francesca Tavano, Anna Panza, Antonio Merla, Giuseppe Biscaglia, et al.SDS-gel followed by transfer to nitrocellulose membrane. Membranes were probed with antiPRKG1 (1:500, #3248, Cell Signaling, Dellaertweg, The Netherlands) and antiGAPDH (1:1000, sc-47724 Santa Cruz, CA, USA)

(17) Role of Endothelin-1 in Right Atrial Arrhythmogenesis in Rabbits with Monocrotaline-Induced Pulmonary Arterial HypertensionInternational Journal of Molecular SciencesSeptember 20, 2022Yen-Yu Lu, Fong-Jhih Lin, Yao-Chang Chen, Yu-Hsun Kao, Satoshi Higa, Shih-Ann Chen, Yi-Jen Chenprobed with primary antibodies against connexin 43 (no. 610062; BD Biosciences, Oxford, UK), PKG (no. 3248; Cell Signaling, Beverly, MA, USA), ROCK1 (no. 4035; Cell Signaling, Beverly, MA, USA), Akt (no. 4685)

(18) Cystathionine γ-lyase promotes estrogen-stimulated uterine artery blood flow via glutathione homeostasisRedox biologyApril 1, 2021Bok R, Guerra DD, Lorca RA, Wennersten SA, Harris PS, Rauniyar AK, Stabler SP, MacLean KN, Roede JR, Brown LD, et al.[35,37]. Primary antibodies detected cyclic GMP-dependent kinase-1 (PKG1; Cell Signaling Technology #3248S, 1:1000), cystathionine β-synthase (CBS; Proteintech #14787-1-AP, 1:2000), cystathionine γ-lyase (CSE;

(19) The Ablation of VEGFR-1 Signaling Promotes Pressure Overload-Induced Cardiac Dysfunction and Sudden DeathBiomoleculesMarch 17, 2021Tirronen A, Downes NL, Huusko J, Laakkonen JP, Tuomainen T, Tavi P, Hedman M, Ylä-Herttuala S.mTOR (2983, Cell Signaling, Danvers, MA, USA), p-mTOR (5536, Cell Signaling, Danvers, MA, USA), PKG-1 (3248, Cell Signaling, Danvers, MA, USA), PKCα (ab32376, Abcam, Cambridge, UK), p- PKCα (ab76016, Abcam, Cambridge,

(20) The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathwayCell death & diseaseFebruary 4, 2021Xiang T, Yuan C, Guo X, Wang H, Cai Q, Xiang Y, Luo W, Liu G.Invitrogen), Vimentin (ab92547, abcam), Snail (26183-1-AP, Proteintech), sGC (ab189176, abcam), PKG1 (3248, CST), PKG2 (55138-1-AP, Proteintech), PDE5A (ab28761, abcam), VASP (13472-1-AP, Proteintech), Phospho-VASPSer239

Be the First to Write a Review!

PKG-1 (C8A4) Rabbit mAb

PKG-1 (C8A4) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (53)

Reviews

PKG-1 (C8A4) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map