CTCF Antibody 2899S from Cell Signaling Technology

Product Specs
ItemCTCF Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2899S
Quantity100 µl
ApplicationsW, IP, IF-IC, ChIP
ReactivityH, M, R, Mk
TargetCTCF
HostRabbit
NCBI Full Gene NameCCCTC-binding factor
ClonalityPolyclonal
Research AreaChromatin Regulation / Nuclear Function
NCBI Gene AliasesCFAP108, FAP108, MRD21
Molecular Weight140

Questions and Answers

Can this CTCF antibody be used for ChIP assays in my research on gene regulation?

Yes, the CTCF antibody has been shown to be effective in Chromatin Immunoprecipitation (ChIP) assays, as demonstrated in studies where it was utilized to analyze CTCF binding sites and their regulatory roles in various contexts, including enhancer-promoter interactions and transcription factor binding dynamics.

What species can I use this antibody on for my experiments?

The CTCF antibody is reactive with several species, including human, mouse, rat, and non-human primates, making it suitable for a variety of biological studies across different model organisms.

Is there any evidence of this antibody being used in studies related to cancer?

Yes, the CTCF antibody has been cited in research involving cancer, such as its role in regulating NOTCH1 expression in rhabdomyosarcoma, where it was shown to interact with SNAI2 to maintain a sub-topologically associating domain boundary essential for tumor cell viability.

How has this antibody been utilized in studies involving transcription factor interactions?

The CTCF antibody has been employed in studies to investigate its interactions with other transcription factors, such as in the context of enhancer loop reprogramming in melanoma, where it was crucial for understanding the regulatory networks that control gene expression in cancer cells.

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(1) Highly efficient chromatin conformation capture with post-enrichment in single cells by HiChewGenome BiolApril 27, 2026Zhichao Chen, Yeming Xie, Chen Zhang, Chen Tan, Fengying Ruan, Wenfang Chen, Meng Luo, Mei Guo, Zhe Xie, Huimin Cao, et al.wet transfer method. The membrane was incubated with an Anti- CTCF antibody (Cell signaling, Cat. No.2899) and Anti-beta Actin antibody (abcam, Cat. No. ab8227) as the primary antibody, followed by an HRP-conjugated

(2) NANP targeting radiosensitizes glioblastoma through TNFR1 sialylation-driven mesenchymal shiftNature CommunicationsMarch 18, 2026Yingwen Ding, Ze-Yan Zhang, Ravesanker Ezhilarasan, Aram S. Modrek, Melanie Graciani, Jerome Karp, Graysen McManus, Ananya Jambhale, Erik P. Sulmanto manufacturer’s instruction. Immunoblots were performed following nuclear extraction. CTCF (CST, 2899) was used as the indicator of nuclear fraction and Tubulin (BioRad, 12004166) was used as the indicator

(3) Chromatin architecture and physical constriction cooperate in phenotype switching and cancer cell disseminationbioRxivFebruary 8, 2026Pietro Berico, Cody Dunton, Luay Almassalha, Amanda Flores Flores-Yanke, Karla I. Medina, Nicolas Acosta, Tara Muijlwijk, Catherine Do, Soobeom Lee, Sharon N Edmiston, et al.western blot input. Next, between 5-10μg of primary antibodies against CTCF (Cell Signaling Technology, #2899), FOSL1 (Cell Signaling Technology, #5281), FOSL2 (Cell Signaling Technology, #19967), c-JUN (Cell Signaling Technology

(4) CTCF maintains centromere function and mitotic fidelityJournal of Cell ScienceFebruary 1, 2026Erin Walsh, Thomas Laskarzewski, Thomas J. Maresca, Andrew D. Stephensdilutions were created using BSA in PBS. The primary antibodies used were: anti-CTCF antibody (rabbit, 2899, Cell Signaling Technologies) at 1:100; anti-CENP-E antibody (mouse, 39619, Active Motif) at 1:1000;

(5) The METTL3-YTHDC1 axis mediates architectural RNA m6A modification to modulate the integrity of chromatin TADs in MLLr + AML genomeMolecular CancerDecember 22, 2025Ruishuang Fu, Wenjuan Yu, Rongjie Zhao, Huimin Yang, Beibei Cao, Chunfei Dai, Haoyue Qianjiang, Yujun Xia, Lubo Wang, Jianrong Lu, et al.with 2–10 ug anti-YTHDC1, METTL3, CTCF, m6A (MeRIP), or IgG (Anti-CTCF, Cell Signaling Technology Cat# 2899; Anti-METTL3, Abcam Cat# ab195352; Anti-m6A, Abcam Cat# ab151230; Anti-YTHDC1, Abcam Cat#ab122340; Anti-IgG,

(6) 3D enhancer architecture coordinated by CTCF determines immune-related gene expression patterns via RNA polymerase II pause-release in CD4+ T cellsNucleic Acids ResDecember 11, 2025Eun-Chong Lee, Kyungwoo Kim, Sugyung Kim, Mikyoung Kim, Hyoung-Pyo Kimmembrane was incubated with primary antibodies against α-tubulin (sc32293) from Santa Cruz and CTCF (2899), p-STAT5 (9351), and STAT5 (94 205) from Cell Signaling Technology, followed by incubation with horseradish

(7) Jpx RNA controls Xist induction through spatial reorganization of the mouse X-inactivation centerDevelopmental CellNovember 3, 2025Hyun Jung Oh, Priyojit Das, Roy Blum, Andrea J. Kriz, Hun-Goo Lee, Yong-Woo Lee, Jeannie T. LeeTable S2. Antibodies The following antibodies were used for ChIP: CTCF (Cell Signaling Technology, 2899S), REX1 (Abcam, ab28141), H3K4me1 (Thermo Fisher Scientific, 710795), H3K27ac (EpiCypher, 13-0045), EZH2

(8) Differential regulation of mesoscale chromosome conformations in osteoblasts and osteosarcomaGenome BiologySeptember 26, 2025Madhoolika Bisht, Yu-Chieh Chung, Siou-Luan He, Sydney Willey, Benjamin D. Sunkel, Meng Wang, Benjamin Z. Stanton, Li-Chun TuPlus gels) and transferred to PVDF membranes (Bio-rad). Rabbit polyclonal antibodies against CTCF (Cat#2899S), H2B-V119 (Cat#8135S), H3 (Cat#9715S), and rabbit monoclonal antibody against H2A-D603A (Cat#12349S)

(9) JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow TransplantationbioRxivSeptember 11, 2025Wentao Han, Hassan Bjeije, Hamza Celik, Michael Rettig, Nancy Issa, Andrew L. Young, Yanan Li, Infencia Xavier Xavier Raj, Christine R. Zhang, Aishwarya Krishnan, et al.antibodies were used for profiling chromatin-bound factors: anti-CTCF (Cell Signaling Technology, #2899S), EZH2 (Abcam, #ab3748; CST, #5246S), H2AK119ub1 (CST, #8240S), histone H3 (CST, #4499S), H3K27ac (CST,

(10) RNA-binding proteins mediate the maturation of chromatin topology during differentiationNature Cell BiologySeptember 8, 2025Bondita Dehingia, Małgorzata Milewska-Puchała, Marcin Janowski, Mahmoud-Reza Rafiee, Misbah Abbas, Aleksandra Piotrowska, Jan Senge, Piotr Blaut, Dietrich Walsh, Jacqueline Severino, et al.primary antibodies at the following concentrations: anti-CTCF (1:2,000, Cell Signaling Technology, 2899S), anti-Fus (1:10,000, Bethyl, A300-294A), anti-Ddx5 (1:5,000, Bethyl, A200-523A) anti-Nono (1:1,000,

(11) Helicobacter hepaticus promotes hepatic steatosis through CdtB-induced mitochondrial stress and lipid metabolism reprogrammingNat CommunAugust 26, 2025Shanhao Jin, Liqi Zhu, Ruoyu Bao, Linghan Yang, Tinglong Zhuang, Liyou Lian, Tao Wang, Jun Yin, Shilei Zhang, Lei Zhou, et al.9205S, CST), anti-P70S6 (1:1000, 9202S, CST), anti-β-actin (1:5000, 4967S, CST) and anti-CTCF (1:1000, 2899S, CST). The membranes were then incubated with horseradish peroxidase-conjugated secondary antibodies

(12) Direction and modality of transcription changes caused by TAD boundary disruption in Slc29a3/Unc5b locus depends on tissue-specific epigenetic contextEpigenetics ChromatinAugust 12, 2025Paul Salnikov, Polina Belokopytova, Alexandra Yan, Emil Viesná, Alexey Korablev, Irina Serova, Varvara Lukyanchikova, Yana Stepanchuk, Nikita Torgunakov, Savelii Tikhomirov, et al.Protein A magnetic beads was washed in PBS, combined with 5 µg target antibodies (Cell Signaling Technology #2899), and incubated for 2 h at 40 C with slow rotation. Beads were removed and pre-cleared chromatin was

(13) CTCF Represses CIB2 to Balance Proliferation and Differentiation of Goat Myogenic Satellite Cells via Integrin α7β1-PI3K/AKT AxisCellsAugust 5, 2025Changliang Gong, Huihui Song, Zhuohang Hao, Zhengyi Zhang, Nanjian Luo, Xiaochuan Chenfragment chromatin into 200–500 bp fragments, followed by immunoprecipitation using an anti-CTCF antibody (2899S, Cell Signaling Technology, Danvers, MA, China). Input controls, representing 10% of the total sample

(14) Coordinated gene expression within sustained STAT3‐associated chromatin conformations contributes to hepatocellular carcinoma progressionCancer CommunicationsJuly 26, 2025Sunyoung Jang, Sumin Yoon, Hyeokjun Yang, Nayun Choi, Su‐Hyang Han, Lark Kyun Kim, Hyoung‐Pyo Kim, Jong Hoon Park, Daeyoup Lee, Kyung Hyun YooCell Signaling, Danvers, MA, USA), p‐STAT3 (Ser727) (9134, Cell Signaling), STAT3 (4904, Cell Signaling), CTCF (2899, Cell Signaling), α‐tubulin (3873, Cell Signaling), Lamin A/C (sc‐376248, Santa Cruz), and ß‐actin (ab8227,

(15) Systematic comparison and base-editing-mediated directed protein evolution and functional screening yield superior auxin-inducible degron technologyNat CommunJuly 18, 2025De Xing, Tao Bai, Ozlem Neyisci, Seyedeh Zahra Paylakhi, Alexander J. Duval, Yasemin Tekin, Mazhar Adliovernight at 4 °C. Primary antibodies including RAD21 (Cell Signaling, 4321S), CTCF (Cell Signaling, 2899S), DNMT3B (Cell Signaling, 67259), RBAP46/RBAP48 (Cell Signaling, 9067), U2AF2 (Cell Signaling, 79462S),

(16) Heat shock induces alternative polyadenylation through dynamic DNA methylation and chromatin loopingCell stress & chaperonesJuly 1, 2025Fink EE, Zhang Y, Santo B, Siddavatam A, Ou R, Nanavaty V, Lee BH, Ting AH.antibodies and their respective dilutions are as follows: CTCF antibody (Cell Signaling Technology #2899) at 1:25 dilution, YY1 antibody (Cell Signaling Technology #46395) at 1:50 dilution, ATF6 antibody (Cell Signaling Technology

(17) CTCF maintains pericentromere function and mitotic fidelitybioRxivJune 3, 2025Erin Walsh, Andrew D. Stephenstemperature. Primary antibody dilutions were created using BSA in PBS. The primary antibodies used were CTCF Rabbit (2899, Cell Signaling Technologies) 1:100, CENP-E mouse (39619, Active Motif) 1:1000, ACA human (HCT-0100

(18) Cohesin and condensin regulate chromosome topology and play an essential role in maintaining pluripotency in embryonic stem cellsSci RepMarch 22, 2025Eui-Hwan Choi, Keun P. Kimanti-SMC4 (Abcam, Cat. 17958), anti-NCAPH2 (Abcam, Cat. ab221722), anti-CTCF (Cell signaling, Cat. #2899), anti-PCNA (Santa Cruz, Cat. 56), anti-GAPDH (Abcam, Cat. ab8245), anti-α-tubulin (Abcam, Cat. ab4074),

(19) Interplay Between TGFβ1 Signaling and Cancer-Testis Antigen MAGEB2: A New Thorn in Cancer's Side?International journal of molecular sciencesMarch 9, 2025Colemon A, Romney CV, Jones AD, Bagsby C, Jackson R, Ramanathan S.grown at 37 °C with 5% CO2 unless otherwise indicated. 4.2. Antibodies CTCF antibody (Cell Signaling, 2899) (Danvers, MA, USA), GABP-a (Santa Cruz Biotech, sc-28312) (Dallas, TX, USA), ELF1 (Santa Cruz Biotech,

(20) The GIP receptor activates futile calcium cycling in white adipose tissue to increase energy expenditure and drive weight loss in miceCell MetabolismJanuary 7, 2025Xinxin Yu, Shiuhwei Chen, Jan-Bernd Funcke, Leon G. Straub, Valentina Pirro, Margo P. Emont, Brian A. Droz, Kyla AI. Collins, Chanmin Joung, Mackenzie J. Pearson, et al.antibodies, SERCA2 antibodies (1:100, #9580; Cell Signaling), or perilipin antibodies (1:1000; Cat# 50-125-2899, Fischer Scientific), then incubated overnight at 4°C. Fluorescent-labeled secondary antibodies were

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CTCF Antibody

CTCF Antibody from Cell Signaling Technology

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CTCF Antibody from Cell Signaling Technology

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