His-Tag (D3I1O) XP ® Rabbit mAb 12698S from Cell Signaling Technology

Product Specs
ItemHis-Tag (D3I1O) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $386.00 Inquire
Catalog Number12698S
Quantity100 µl
ApplicationsW, W-S, IP, IF-IC, FC-FP, ChIP
ReactivityAll
HostRabbit
ClonalityMonoclonal
Research AreaRelated Products
CloneD3I1O

Questions and Answers

Can this His-Tag antibody be used for immunoblotting in my experiments?

Yes, the His-Tag (D3I1O) XP® Rabbit mAb has been utilized in immunoblotting applications, as evidenced by its use in various studies, including those that specifically mention its application at a dilution of 1:1000 for Western blotting ().

Is this antibody effective for immunofluorescence staining?

The His-Tag antibody has been shown to work effectively in immunofluorescence applications, with reports indicating its use at a dilution of 1:100 for this purpose ().

What types of samples can I use this antibody on?

This antibody recognizes recombinant proteins containing the 6xHis epitope tag and has been shown to work across a wide range of species, including human, mouse, zebrafish, and various other vertebrates and invertebrates, making it versatile for different experimental setups.

Are there any specific studies that have cited the use of this antibody in apoptosis research?

Yes, the His-Tag antibody has been referenced in studies investigating apoptosis, such as in the context of exogenous introduction of caspases, where it was used to analyze protein levels and interactions in apoptotic pathways ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Characterization of a novel Mycobacterium tuberculosis serine protease Rv1815 in regulating bacterial metabolism and macrophage intracellular survivalCurrent Research in Microbial SciencesApril 20, 2026Qishun Feng, Qiao Lin, Zhang Xia, Junyan He, Siqi Li, Yuzhong Xu, Yan Ma, Guoliang Zhangcells was subsequently verified by western blot analysis using an anti–6×His antibody (CST, Cat. No. 12698S, USA). The expressed cells were disrupted through ultrasound in a lysis buffer composed of 20 mM Tris–HCl,

(2) The tumor suppressor NDRG2 promotes ACC1 proteasomal degradation and inhibits de novo lipogenesis in hepatocellular carcinomaMol BiomedApril 17, 2026Qianqian Shi, Yu Bai, Jiayuan Wang, Mengyao Ru, Kun Zhang, Yan Guo, Zhantao Bai, Lan Shenagainst ACC1 (#4190, 1:1000 for immunoblotting), phospho-AMPKα (#2535, 1:1000 for immunoblotting), His (#12698, 1:100 for IP) and Myc (#2276, 1:1000 for immunoblotting) were purchased from Cell Signaling Technology.

(3) FAT2 inhibits breast cancer cell migration, invasion, and epithelial-mesenchymal transition through transcriptional upregulation of CLDN19Front OncolApril 13, 2026Gangyin Xie, Dengwei Lu, Ziwei Li, Tao Xu#H00149461-M02, 1:500), anti-His-Tag (D3I1O) rabbit monoclonal antibody (Cell Signaling Technology [CST], #12698, 1:1000), anti-Vimentin (CST, #5741, 1:1000), anti-N-cadherin (CST, #13116, 1:1000), anti-E-cadherin

(4) Bio-inspired spatiotemporal cascade reaction liposome for ferroptosis treatment following subarachnoid hemorrhageMater Today BioMarch 3, 2026Zheng Peng, Xiao-Jian Li, Yu-Long Xi, Xiao-Long Zhu, Yue Lu, Yan Zhou, Hui-Ying Yan, Juan Wang, Zheng-Mao Yang, Chun-Hua Hang, et al.Siginaling Technology, 1:1000), anti-β-actin (4967, Cell Siginaling Technology, 1:1000), anti-His tag (12698, Cell Signaling Technology, 1:1000), and anti-Na,K-ATPase α1 (ATPA1, 23565, Cell Signaling Technology,

(5) A single mutation in nonstructural protein 1 is critical for the adaptive evolution of influenza B virusNature CommunicationsMarch 2, 2026Pengtao Jiao, Xiaoxiao Jia, Xiaoyuan Bai, Yuna Zhao, Runshan Lin, Zeqiu Fan, Wenhui Fan, He Zhang, Heqiao Li, Yuhai Bi, et al.(D5F5U) (Cat #12994), Tom20 (D8T4N) (Cat #42406), TIM23 (E1Q7L) (Cat #34822), His-Tag (D3I1O) XP® (Cat #12698), and mouse monoclonal antibodies against Myc-Tag (9B11) (Cat #2276), GAPDH (D4C6R) (Cat #97166), LC3B

(6) Secreted RCN3 acts as an early epithelial-fibroblast mediator via TGFβR1-Smad signaling in post-ALI pulmonary fibrosisCell Communication and SignalingFebruary 9, 2026Xiaoqian Shi, Zhenyan Wang, Fangping Ding, Runlin Z. Ma, Jing Wang, Yingmin Ma, Jiawei Jin(Santa Cruz, sc-37007), MiQumn G25 centrifugal desalting column (Chromsep, 110607), anti-His antibody (CST, 12698), anti-TGFβR1 antibody (Abcam, ab235578), anti-TGFβR2 antibody (Abcam, ab259360), anti-Smad3 (phospho S423 + S425

(7) Targeted stress granule regulation by engineering a non-catalytic O-GlcNAc transferaseNature CommunicationsJanuary 7, 2026Na Wang, Fanjia Hou, Sihui Ma, Silan Liu, Haimei Wei, Weicheng Fang, Ke Zhang, Yun Gereagents Commercial antibodies were used under the following conditions: anti-His (Cell Signaling Technology [CST] #12698; 1:400 for immunofluorescence [IF], 1:1000 for immunoblotting [IB]), anti-Myc (CST #2276; 1:1000 IF/IB),

(8) MET Enhances Amivantamab Binding to EGFR and Antibody‐Dependent Cellular ToxicityCancer ScienceJanuary 1, 2026Shigeki Sato, Neval Yilmaz, Sachiko Arai, Katsuya Sakai, Hiroki Sato, Yuya Murase, Tsukasa Ueda, Hayato Koba, Shigeki Nanjo, Yuichi Tambo, et al.fractions were detected using western blotting. Because EGFR was His‐tagged, an anti‐His‐tag antibody (#12698; Cell Signaling Technology) was used as the primary antibody. The human HGFR/c‐MET antibody (#MAB3582;

(9) Coupling proteostasis and de novo purine biosynthesis of PSMD14 fuels glioblastoma progression and chemoresistanceTheranosticsJanuary 1, 2026Jiazheng Wang, Qun Cao, Zhikai Li, Xuxiu Lu, Zhuo Li, Chenghui Guo, Yuan Pan, Qing Zhang, Wenjie Li, Guo Xiang, et al.Cell Signaling Technology), K63-linkage-specific polyubiquitin (5621S; 1:1,000; Cell Signaling Technology), His-Tag (12698T; 1:1000; Cell Signaling Technology), HA-Tag (3724S; 1:1000; Cell Signaling Technology) and Myc-Tag (2276S;

(10) ENO1-mediated deoxycytidine synthesis and gemcitabine resistance by stabilizing RRM2 in pancreatic cancerCell Death & DiseaseDecember 27, 2025Yongning Li, Hao Wang, Liwen Chen, Yanyu Gong, Dijie Zheng, Futang Li, Changhao Wu, Zhiwei He, Chao Yu(ab134064), and flag (ab205606), all procured from abcam. Additionally, HA (3724), Myc (2276), His (12698), ubiquitin (3936), HRP-conjugated anti-mouse IgG (7076), and HRP-conjugated anti-rabbit IgG (7074)

(11) Mycobacterium tuberculosis PE_PGRS62 Protein Inhibits Type I Interferon Responses to Promote HIV-2 Replication by Directly Interacting With Interferon Regulatory Factor 3The Journal of Infectious DiseasesDecember 20, 2025Chaohu Pan, Hui Xu, Min Huang, Junyan He, Siqi Li, Xiaoyu Tao, Tingzhi Cao, Guoliang Zhang(no. 5483), anti-TBK1 (no. 3504), anti–p-IRF3 (no. 37829), anti-IRF3 (no. 11904), anti-His tag (no. 12698), anti-Flag tag (no. 14793), anti–glutathione S-transferase (GST) (no. 2625), anti-hemagglutinin (HA)

(12) Netrin-1 inhibits the attachment and internalization of Hepatitis B virus for hepatocyte infectionPLoS PathogDecember 17, 2025Ying Wang, Kazuhisa Murai, Atsuya Ishida, Narumi Kawasaki, Kazuyuki Kuroki, Ying-Yi Li, Yuga Sato, Yutaro Miura, Kureha Takara, Lianghao Kong, et al.CST), p-p44/42 MAPK (Erk1/2) (9101; CST), GFP (mFX75; Wako), Na, K-ATPaseα1 (23565; CST), His-tag (12698; CST), anti-rabbit IgG (7074; CST), anti-mouse IgG (7076; CST), VeriBlot for IP Detection Reagent (HRP)

(13) Targeted Intracellular Delivery via Precision Programming of ARRDC1-Mediated MicrovesiclesJ Extracell VesiclesDecember 14, 2025Zhi Qiao, Sengjin Choi, Zunwei Chen, Roland Mason Rodriguez, Qiyu Wang, Zhiping Yang, Samuel A. Theuerkauf, Joseph F. Nabhan, Takao K. Hensch, Christian J. Buchholz, et al.(Nabhan et al. 2012). GFP (CST, 2956S); Vinculin (Abcam, ab129002); CD9 (CST, 1317S); His‐tag (CST, 12698S); AU1 (Bethyl, A190‐125A) and GluA4 (Abcam, ab109431), all at 1:2000 dilution. HRP‐conjugated anti‐rabbit

(14) Extracellular Domain Shedding of TROP2 Activates EGFR Signaling to Drive Prostate Cancer MetastasisCancer researchDecember 1, 2025Liu S, Hsu EC, Aslan M, Garcia-Marques F, Shen M, Hartono AB, Solano F, Le K, Hwang H, Lee CS, et al.(Sino Biological Cat# 10428-MM01, RRID:AB_2860179, 1:100); anti-His-tag (Cell Signaling Technology Cat# 12698, RRID:AB_2744546, 1:100); anti-Fibronectin (Santa Cruz Biotechnology Cat# sc-8422, RRID:AB_627598, 1:100);

(15) Engineered macrophages accumulate in solid tumors and locally deliver immune-activating proteins to inhibit tumor progressionTranslational Cancer ResearchNovember 30, 2025Yan Xu, Yang Zhou, Jiahe WangUK), IFN-γ (1:500, CST, #98139, USA), IL-12 (1:500, Abcam, ab80682, UK), and His-Tag (1:500, CST, #12698, USA). Next, the membranes were incubated with an appropriate secondary antibody at 37 °C for 1 hour.

(16) TAF1 acetyltransferase promotes colorectal carcinoma metastasis by catalyzing β-hydroxybutyrylation of KCTD9OncogeneNovember 27, 2025Jun Su, Miao Zhao, Wanjing Feng, Ying Lin, Xulong Lu, Zuguang Xia, Teng Cheng, Qing Liu, Limin Cheng, Chun Pu, et al.(NICD, #4380), CBP (#7389 T), P300 (#86377), TRIM21 (#92043), HA (#3724), Actin (#4967S) and His (#12698) were from CST (Danvers, MA); anti-β-Hydroxybutyryllysine (#PTM-1201RM) was from PTM BIO (Chicago, USA);

(17) USP14-mediated stabilization of ACTN1 maintains mesenchymal characteristics in glioblastomaCommunications BiologyNovember 25, 2025Wenjin Qiu, Xueping Shi, Ruting Wei, Jiaquan Song, Yuanguo Ling, Yunjia Hu, Shibin Song, Yimin Chen, Hua Yang, Peng Luo, et al.#sc-17829; WB 1:500, IF 1:100), FLAG-tag (Abcam, #ab205606; WB 1:1000), His-tag (Cell Signaling Technology, #12698; WB 1:1000), HA-tag (Abcam, #ab9110; WB 1:1000), YKL-40 (Novus Biologicals, #AF2599; WB 1:1000), Olig2

(18) Structural characterization of zebrafish Ngly2, an ovary-enriched acid PNGase required for egg free glycan productionJournal of Biological ChemistryNovember 5, 2025Akinobu Honda, Katsuhiko Kamada, Junichi Seino, Hiroto Hirayama, Haruhiko Fujihira, Masashi Ueki, Toshiyuki Shiraki, Raymond N. Burton-Smith, Kazuyoshi Murata, Nozomi Ishii, et al.incubated with rabbit anti-His-tag antibody (1/1000 dilution, Cell Signaling Technology; catalog no.: 12698). After incubation with goat anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase

(19) Activating NEDD4L suppresses EGFR-driven lung adenocarcinoma growth via facilitating EGFR proteasomal degradationJ Exp Clin Cancer ResOctober 21, 2025Maojian Chen, Wei Jiang, Jianhua Zhan, Shaoping Zhang, Jiani Zheng, Yihua Huang, Junyi He, Yunpeng Yang, Shen Zhao, Yaxiong Zhang, et al.Cell Signaling Technology; 1:1000), HA-tag (Cat. #2367/3724, Cell Signaling Technology; 1:1000), His-tag (Cat. #12698, Cell Signaling Technology; 1:1000), Ki67 (Cat. #ab16667,Abcam; 1:500), LC3B (Cat. #83506, Cell Signaling Technology

(20) Nanobody Immunolabelling and three-dimensional imaging reveals spatially restricted LYVE1 expression by kidney lymphatic vessels in miceJ NanobiotechnologyOctober 13, 2025Eva Maria Funk, Daniyal J. Jafree, Nils Rouven Hansmeier, Clàudia Abad Baucells, Rose Yinghan Behncke, Gideon Pomeranz, Maria Kolatsi-Joannou, William J. Mason, Dale Moulding, Lauren G. Russell, et al.membrane (1620112, Bio-Rad Laboratories Inc.) and nanobodies identified using a primary anti-His antibody (12698, Cell Signaling Technology, Danvers, MA, USA) and a secondary anti-rabbit antibody (926-32211, LI-COR Biosciences

Be the First to Write a Review!

His-Tag (D3I1O) XP ® Rabbit mAb

His-Tag (D3I1O) XP ® Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (206)

Reviews

His-Tag (D3I1O) XP ® Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map