RUNX2 (D1L7F) Rabbit mAb 12556S from Cell Signaling Technology

Product Specs
ItemRUNX2 (D1L7F) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12556S
Quantity100 µl
ApplicationsW, IP, FC-FP, ChIP, ChIP-seq
ReactivityH, M, R
TargetAML3
HostRabbit
NCBI Full Gene NameRUNX family transcription factor 2
NCBI Gene AliasesAML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA
ClonalityMonoclonal
Research AreaDevelopmental Biology
CloneD1L7F
Molecular Weight55-62

Questions and Answers

Can this RUNX2 antibody be used for immunofluorescence applications?

Yes, the RUNX2 (D1L7F) Rabbit mAb has been shown to work in immunofluorescence applications, as evidenced by its use in studies where it was applied to analyze RUNX2 expression in various cell types, including vascular smooth muscle cells and macrophages.

Is this antibody effective for Western blotting?

The RUNX2 antibody has been utilized successfully in Western blotting, as demonstrated in multiple studies where it was employed to detect RUNX2 protein levels in various experimental setups, including those involving vascular smooth muscle cells and bone mesenchymal stem cells.

What species can this antibody recognize?

The RUNX2 antibody is reactive with mouse, human, and rat samples, making it suitable for studies involving these species.

Are there any specific applications where this antibody has been cited in the literature?

Yes, the RUNX2 antibody has been cited in studies focusing on osteogenic differentiation and vascular calcification, where it was used to assess RUNX2 expression in response to various treatments and conditions, such as in the context of chronic kidney disease and atherosclerosis.

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Role of miR-101a in targeting Cox-2 to attenuate chondrocyte hypertrophic differentiation and osteoarthritis progressionGenes & DiseasesMay 1, 2026Rui Mi, Jinnan Chen, Tianxiang Zhu, Huiqin Bian, Rong Wei, Rushuang Deng, Tiaotiao Han, Qian Wang, Yaojuan Lu, Longwei Qiao, et al.(D223097, Biotechnology, Shanghai, China), MMP13 (ab51072, Abcam, Cambridge, Massachusetts, USA), RUNX2 (#12556, CST, USA), and SOX9 (#82630, CST, USA), which were prepared with primary antibody dilution (Yeasen

(2) Osteogenic and Anti-Inflammatory Effects of Strontium-Loaded Polydopamine on Micro-Arc Oxidized Titanium SurfacesJ Funct BiomaterApril 7, 2026Yiming Yang, Rongpu Liu, Yuqi Zhou, Lingjun Yuan, Zhenxia Li, Qian Liao, Bing Fangat 4 °C with specific primary antibodies, including anti-Runx2 (Cell Signaling Technology, Cat. No. 12556, Danvers, MA, USA), anti-OCN (Proteintech, Cat. No. 23418, Rosemont, IL, USA), anti-ALP (Santa Cruz Biotechnology, Inc

(3) Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling PathwayDose ResponseApril 7, 2026Huahui Bian, Dongyang Zhao, Haoyu Wang, Hao Huang, Youyou Wang, Weibo Chen, Chang Liu, Yinyin Yang, Anqing Wu, Yulong Liuwith primary antibodies targeting RUNX2 (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat. No. 12556), COL1A1 (1:1000, Cell Signaling Technology, Cat. No. 72026), OCN (1:1000, Cell Signaling Technology,

(4) Apolipoprotein C3 exacerbates vascular calcification by promoting ferroptosis via the TLR2/AMPK pathway in vascular smooth muscle cellsRedox BiologyApril 1, 2026Zihao Zhou, Jinxuan Chen, Huan Wang, Yufei Han, Changyun Liu, Qiang Shen, Zhao Dong, Kaikai Lu, Chao Zhai, Yitong Xu, et al.ab209217, Abcam, USA), pAMPK (1:1000, 2535S, CST, USA), tAMPK (1:1000, 5831S, CST, USA), RUNX2 (1:1000, 12556S, CST, USA), NRF2 (1:1000, 12721S, CST, USA), ApoC3 (1:1000, YT5550, Immunoway, USA), BMP2 (1:1000, YM8215,

(5) Uremic toxin p‑cresyl sulfate enhances the calcification of aortic valvular interstitial cells via klotho/sirtuin‑1 signalingMol Med RepApril 1, 2026Shao-Jung Li, Tzu-Yu Cheng, Yu-Hsun Kao, Wei-Yu Chen, Cheng-Chih Chung, Nguyen Ngoc Trang, Pao-Huan Chen, Yi-Jen Chentemperature and incubated overnight at 4°C with primary antibodies against RUNX2 (1:2,000 dilution; cat. no. 12556; Cell Signaling Technology), acetylated-NF-κB (1:2,000 dilution; acetyl-NF-κB; cat. no. 3045; Cell Signaling Technology

(6) A Standard Herbal Formula, CGAC, Attenuates Bone Loss by Normalizing Low-Bone Turnover Stagnation in an Orchiectomy-Induced Mouse ModelPharmaceuticals (Basel)March 31, 2026Dong-Cheol Baek, Min-Young Chae, Tae-Wook Woo, Chang-Gue Son, Eun-Jung LeeCell Signaling Technology, Danvers, MA, USA), AMPKα (1:1000, #2532, Cell Signaling Technology), Runx2 (1:1000, #12556S, Cell Signaling Technology), β-catenin (1:1000, ab32572, Abcam, Cambridge, UK), phospho-GSK3β (Ser9)

(7) Dnmt3a-dependent de novo DNA methylation enforces lineage commitment and preserves functionality of memory Th1 and Tfh cellsCell ReportsMarch 24, 2026Bryant Perkins, Camille L. Novis, Andrew Baessler, Linda M. Sircy, Monyca M. Thomas, Malia Harrison-Chau, Andrew W. Richens, Bryce Fuchs, Nguyen X. Nguyen, Kaitlyn Flint, et al.BV421,BD Horizon,catalog # 564270; clone # 5E7; RRID: AB_2738720 anti Runx2 PE,Cell Signaling,catalog # 12556; clone #D1L7F; RRID: AB_2800297 anti Runx3 BV421,BD Horizon,catalog # 565742; clone #R3-5G4; RRID:

(8) Bone Morphogenetic Protein (BMP) 9 Outperforms BMP2 in Osteogenesis and Osseointegration: In Vitro and In VivoClin Implant Dent Relat ResMarch 10, 2026Hee-Seung Han, Dong Woo Lee, Yu-Bin Kim, Sanghui Seok, Jeong Yeon Park, Sungtae Kim, Jue Yeon Lee, Yoon Jeong Park, Chong Pyung Chung, Young-Dan Choanti‐phospho‐SMAD1 (Ser463/465)/SMAD5 (Ser463/465)/SMAD9 (Ser465/467) (D5B10, #13820), anti‐COL1A1 (#66948), anti‐RUNX2 (#12556), anti‐Osteocalcin (#59757), anti‐GAPDH (#2118) (Cell Signaling Technology, Beverly, MA); as well as

(9) Local delivery of OSK factors enables partial cellular reprogramming to mitigate osteoarthritis and cartilage fibrosisExperimental & Molecular MedicineMarch 5, 2026Yi-Wei Liu, Jing-Tao Zou, Jiang-Shan Gong, Ling Jin, Jia Cao, Ze-Hui He, Yu-Xuan Qian, Xin Wang, Mei-Dan Wan, Xin-Yue Hu, et al.compound. Primary antibodies against SOX2 (14-9811-82, Thermo Fisher), IL-1 (GB11113, Servicebio), RUNX2 (12556, CST), OSX (sc393325, Santa Cruz) and OCN (GB11233, Servicebio) were utilized. Subsequently, secondary

(10) Contactin-2 protects against aortic valve calcification via osteogenic differentiation inhibitionScientific ReportsMarch 4, 2026Zhongxing Zhou, Ruming Shen, Shuaijie Chen, Hailin Zhang, Longqing Chen, Xiuzhu Weng, Xiaoyan Lin, Dajun Chaitransferred to a PVDF (Millipore) membrane. The primary antibodies used were as follows: anti-RUNX2 (#12556T, CST, 1:1500) rabbit mAb, anti-ALP (#30971S, CST, 1:1500) rabbit mAb, anti-CNTN2 (#67089-1-Ig, Proteintech,

(11) Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2-Induced Ossification of the Annulus Fibrosus CellsJOR SpineMarch 3, 2026Hisakazu Shitozawa, Ryo Nakamichi, Aki Yoshida, Masataka Ueda, Taichi Saito, Koji Uotani, Yoshiaki Oda, Ryo Takatori, Kazutaka Yamashita, Toshifumi Ozakiwere used: Runx2 (1:1000 dilution, Rabbit Anti‐Runx2, Cell Signaling Technology, Danvers, MA, Cat. #12556), F‐Actin (Anti‐Actin hFAB Rhodamine, Bio‐Rad, Cat. #12004163), and IRDye goat anti‐rabbit IgG (LI‐COR

(12) Canonical BAF chromatin remodeling complex specifies stem cell fate via cell-type-specific co-factor recruitmentNature CommunicationsMarch 3, 2026Mingyi Zhang, Jifan Feng, Tingwei Guo, Lin Meng, Fei Pei, Lu Gao, Yuchen Yang, Thach-Vu Ho, Yang Chaiab15580), β-galactosidase (β-GAL) (1:100, Abcam, ab9361), RUNX2 (1:100, Cell Signaling Technology, 12556), and Phospho-Histone H2A.X (Ser139) (Cell Signaling Technology, 2577). RNAscope in situ hybridization

(13) MSC-mimicking nanovesicle embedded bio-adhesive hydrogel for dual immunomodulation and osteogenesis to promote maxillofacial bone regenerationBioact MaterMarch 2, 2026Tingting Yu, Jiale Yan, Jingwei Wang, Zhiqiang Song, Rushui Bai, Yuntao Lu, Qin Niu, Tianyu Cai, Bing Han, Xing Wang, et al.ATPase (1:1000, Cat. sc28800, Santa, USA), BMP2 (1:1000, Cat. ab284387, Abcam, UK), RUNX2 (1:1000, Cat. 12556, Cell Signaling Technology, USA) and β-actin (1:10000, Cat. T0022, Affinity, China) overnight at 4 °C,

(14) TIGAR promotes osteogenic differentiation and ameliorates glucocorticoid-induced osteoporosis via autophagy-Nrf2-ROS axisGenes & DiseasesMarch 1, 2026Dingmei Zhang, Feng Ding, Yizhong Wang, Jie Cheng, Jiaxing Zhu, Shiyu Liu, Xin Wang, Zheng-Hong Qin, Lili Renanti-mouse TIGAR (1:500, Santa Cruz, sc166290), anti-rabbit Runx2 (1:1000, Cell Signaling Technology, #12556), anti-rabbit sp7 (1:1000, Abcam, ab209484), anti-rabbit Nrf2 (1:1000, Zen-Bio, 380773), anti-rabbit

(15) Dual functional properties of a probiotic biofilm-decorated bone substitute to combat infection and promote osteoimmunomodulationBioactive MaterialsMarch 1, 2026Shiyuan Song, Wen Zhang, Hongmei Zhuang, Wei Wei, Shuyu Cheng, Dan Qiao, Yin Xiao, Yangheng Zhang, Fuhua Yanprimary antibodies used included antibodies against Runt-related transcription factor 2 (Runx2) (1:500, #12556, CST, MA, USA), PI3K (1:1000, #4257, CST, MA, USA), p-PI3K (1:1000, #4228, CST,MA, USA), AKT (1:1000,

(16) Magnesium ions facilitate osteogenic differentiation and intervertebral fusion via m6A methylation of RhoA mRNAJournal of Orthopaedic TranslationMarch 1, 2026Haocheng Xu, Linli Li, Fan Zhang, Minghao Shao, Chenyan Li, Yitong Xue, Dachuan Li, Zhidi Lin, Zhaoyang Gong, Jiongdong Wu, et al.anti-METTL3 (1:200; Cell Signaling Technology, 86132S), and anti-Runx2 (1:200; Cell Signaling Technology, 12556S). The following day, the sections were incubated at 37 °C for 1 h with fluorescent-conjugated secondary

(17) DEC1 regulates osteoblast proliferation and differentiation via the RUNX2 signaling pathway: Insights into the molecular mechanism of DEC1-mediated transcriptional regulation in bone metabolismOpen Life SciencesFebruary 24, 2026Wenjie Qian, Kai Mei, Lei Zhu, Jinpeng Lv, Changjun Yun(Diagenode). Immunoprecipitation was carried out using anti-RUNX2 antibody (Cell Signaling Technology, Cat# 12556) and protein A/G magnetic beads (Thermo Fisher Scientific, Cat# 88803). Immunoprecipitated DNA was purified

(18) Poly-l-lactic acid electrospun membrane with specific morphology promotes bone regeneration through macrophage reprogrammingJournal of NanobiotechnologyFebruary 19, 2026Daiyuan Tang, Yunrong Xu, Zaitian Huang, Zhenping Xiao, Chenxun Sun, Jingyu Li, Fei He, Bing Han, Chen ZhuUSA) as markers for stem cells, along with OPN (#88742; Cell Signaling Technology, MA, USA), RUNX2 (#12556; Cell Signaling Technology), and BMP2 (#ab284387; Abcam) which are involved in osteogenesis. Moreover,

(19) Caldesmon-1–mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cellsScientific ReportsFebruary 17, 2026Munenori Komoda, Tomohisa Sakaue, Yasuhisa Nakao, Hiroshi Sakamoto, Takuma Fukunishi, Tomohide Higaki, Jun Aono, Hirotsugu Kurobe, Mie Kurata, Takashi Nishimura, et al.(Proteintech, Cat. No. 11187-1-AP, 1:500 dilution), anti-RUNX2 (Cell Signaling Technology, Catalogue no. 12556 S, 1:500 dilution), and anti-α-Tubulin (FUJIFILM Wako, Catalogue no. 013-25033, 1:1000 dilution). After

(20) Astaxanthin enhances migration, Type I and Type III collagen production, and osteogenic differentiation in periodontal ligament fibroblasts under hyperglycemic conditionsBMC Oral HealthFebruary 10, 2026Varunya Chantadul, Wongsathorn Aphichartsereekul, Kanokwan Charoonpatrapong, Kajohnkiart Janebodin, Jirattikarn Kaewmuangmoonprepared in TBST. Membranes were then incubated with anti-RUNX2 (1:1000, D1L7F Rabbit Monoclonal Antibody #12556, Cell Signaling Technology) and anti-Nrf2 (1:1000, D1Z9C Rabbit Monoclonal Antibody #12721, Cell Signaling Technology

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RUNX2 (D1L7F) Rabbit mAb

RUNX2 (D1L7F) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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RUNX2 (D1L7F) Rabbit mAb from Cell Signaling Technology

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