HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) 11846S from Cell Signaling Technology

Product Specs
ItemHA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate)
CompanyCell Signaling Technology
Price $450.00 Inquire
Catalog Number11846S
Quantity400 µl
ApplicationsIP
Conjugate/TagMagnetic Bead
HostRabbit
ClonalityMonoclonal
Research AreaRelated Products
ReactivityAll
IsotypeIgG
CloneC29F4

Questions and Answers

Can this HA-tag antibody be used for immunoprecipitation in C. elegans studies?

Yes, the HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) has been successfully utilized for immunoprecipitation in studies involving C. elegans, as demonstrated in research where the antibody was employed to pull down proteins for mass spectrometry analysis (, ).

What types of proteins can this antibody detect?

The HA-Tag antibody is designed to detect exogenously expressed proteins containing the HA epitope tag, which is commonly used in various species, including human, mouse, zebrafish, and even invertebrates like C. elegans and Drosophila (, ).

Is this antibody effective for use in mammalian cell lines?

Yes, the HA-Tag antibody has been shown to work effectively in mammalian cell lines, as evidenced by its application in immunoprecipitation assays for various proteins, including those involved in cancer research (, ).

Are there any specific applications or experimental contexts where this antibody has been cited?

The HA-Tag antibody has been cited in multiple studies for applications such as immunoprecipitation and Western blotting, particularly in the context of studying protein interactions and post-translational modifications in various biological systems (, , ).

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Website: Technical Support

(1) Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cellsNucleic Acids ResearchFebruary 23, 2026Huanhuan Feng, Zhixue Li, Hongdao Zhang, Yuli Zheng, Beiying Xu, Yao Zhang, Lin Zou, Ligang Wuadapter ligation Fifty microliters of HA antibody-coupled magnetic beads (Cell Signaling Technology, 11846) were added to the lysate and rotated at 4°C for 1 h. The rotated mixture was subsequently placed on

(2) HAT1 as a Lactyltransferase to Promote DNA Repair Through RPA1 Lactylation in GlioblastomaInt J Biol SciJanuary 22, 2026Yuzu Zhao, Jiang He, Zhiying Zhou, Tangmin Lai, Siwei Zeng, Nan Li, Yu He, Wei Zhou, Yongzhong Wu, Bo XuShanghai, China). For co-Immunoprecipitation, HA tag (C29F4) rabbit mAb (Magnetic bead conjugate) (#11846S) was purchased from CST (Shanghai, China). Anti-FLAG® M2 magnetic beads (#M8823) was obtained from Sigma

(3) HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinomaCell Death & DiseaseNovember 21, 2025Jiang He, Tangmin Lai, Yuzu Zhao, Zhiying Zhou, Liu Zhou, Dan Tao, Haonan Yang, Nan Li, Yu He, Shuheng Yang, et al.obtained from HUABIO. For co-Immunoprecipitation, HA tag (C29F4) rabbit mAb (Magnetic bead conjugate) (#11846S) was purchased from CST. Anti-FLAG® M2 magnetic beads (#M8823) were obtained from Sigma Aldrich. Nano-Traps,

(4) The tegument protein VP22 of pseudorabies virus inhibits cGAS condensation by inducing nuclear-to-cytoplasmic translocation of DDX21PLOS PathogensSeptember 29, 2025Kesen Liu, Wandi Cao, Hanhua Zhang, Xingmiao Yang, Chengyue Wu, Xingya Wang, Qian Sun, Jianxiong Guo, Ting Zhang, Yan Liu, et al.beads (Santa Cruz Biotechnology, sc-500,772), or anti-HA magnetic beads (Cell Signaling Technology, 11846) at 4°C for 6 hours. The beads were then washed three times with wash buffer (50 mM Tris-HCl, pH 7.4;

(5) BQ323636.1 Employs the AR-CCRK Axis to Modulate the Expression of KU70 to Interfere with Non-Homologous End Joining Mediated DNA Repair Mechanism †CellsAugust 29, 2025Ho Tsoi, Zi-Qing So, Ellen P. S. Man, Chan-Ping You, Koei Ho-Lam Cheung, Yin-Suen Tse, Wing-Lok Chan, Ui-Soon KhooMA, USA); anti-HSP90 (1:5000; #4874, Cell Signaling Technology, Danvers, MA, USA), anti-HA (1:2000; #11846, Cell Signaling Technology, Danvers, MA, USA), anti-ATR (1:1000; #2790, Cell Signaling Technology, Danvers,

(6) Phosphatase activity is dispensable for PRL-3-mediated oncogenesis and tumor progressionbioRxiv : the preprint server for biologyMay 18, 2025Jolly JT, Al-Hamaly MA, Smith CN, Spielmann HP, Blackburn JS.incubated for one hour at room temperature with BSA-blocked HA-Tag (C29F4) magnetic beads (Cell Signaling, 11846S, Lot #9) (RRID:AB_2665471) in IP buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP-40 [Abcam, ab142227],

(7) Multiplexed, scalable analog recording of gene regulation dynamics over weeks using intracellular protein tapesbioRxivMay 11, 2025Lirong Zheng, Yixiao Yan, Bingxin Zhou, Jormay Lim, Dongqing Shi, Bobae An, BumJin Ko, Emily Klyder, Sethuramasundaram Pitchiaya, Denise J. Cai, et al.antiOLLAS (Invitrogen MA5–16125), anti-FLAG (Invitrogen 740001), and anti-HA (Cell Signaling Technology 11846S; Invitrogen PA5–33243). Fluorescent secondary antibodies (1:500 for immunofluorescence of cultured cells):

(8) An in vivo "turning model" reveals new RanBP9 interactions in lung macrophagesCell Death DiscovApril 13, 2025Yasuko Kajimura, Shuxin Dong, Anna Tessari, Arturo Orlacchio, Alexandra Thoms, Maria Concetta Cufaro, Federica Di Marco, Foued Amari, Min Chen, Shimaa H. A. Soliman, et al.for 1 h at room temperature. The pre-cleared lysate was then mixed with anti-HA magnetic beads (CST: #11846) or anti-V5 magnetic beads (CST: #53475), or IgG magnetic beads (CST: #5873) and incubated rotating

(9) Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1Commun BiolMarch 3, 2025Fei Wu, Yanqiong Chen, Xueqiu Chen, Danni Tong, Jingru Zhou, Zhendong Du, Chaoqun Yao, Yi Yang, Aifang Du, Guangxu Main this study were purchased from Cell Signaling Technology, Inc. (including HA-Tag (C29F4; cat no. 11846S), DYKDDDDK Tag (D6W5B; cat no. 15008S) and Myc-Tag (71D10; cat no. 2278S) rabbit mAb) (Danvers, MA,

(10) The TRIM33 Bromodomain Recognizes Histone Lysine LactylationACS Chemical BiologyDecember 20, 2024Raymundo Nuñez, Paul F. W. Sidlowski, Erica A. Steen, Sarah L. Wynia-Smith, Daniel J. Sprague, Robert F. Keyes, Brian C. Smithimmunoprecipitation, 15 μL of HA-Tag [C29F4] rabbit mAb magnetic bead conjugate (Cell Signaling Technology, #11846) was added to the remainder of nuclear fraction lysate, and the mixture was incubated for ~16 h with

(11) UBR-5 and UBE2D mediate timely exit from stem fate via destabilization of poly(A)-binding protein PABP-2 in cell state transitionProceedings of the National Academy of SciencesOctober 22, 2024Jose Francisco Calva Moreno, George Jose, Yi M. Weaver, Benjamin P. Weaverand the lysate was incubated with HA antibody conjugated magnetic beads (Cell Signaling Technology, 11846S). The beads were washed with lysis buffer and eluted in 2.5% BME SDS loading buffer at 95 °C. The eluate

(12) USP36 inhibits apoptosis by deubiquitinating cIAP1 and survivin in colorectal cancer cellsJournal of Biological ChemistryJuly 1, 2024Bao Gao, Yuan Qiao, Shan Zhu, Ning Yang, Shan-Shan Zou, Yong-Jun Liu, Jingtao Chencomplexes were immunoprecipitated with Myc-Tag (Cat# 5698; Cell Signaling Technology), HA-Tag (Cat# 11846; Cell Signaling Technology), anti-Flag (M2) magnetic beads (Cat# M8823; Sigma-Aldrich), or the indicated

(13) The DNA helicase FANCJ (BRIP1) functions in double strand break repair processing, but not crossover formation during prophase I of meiosis in male micePLoS geneticsFebruary 1, 2024Horan TS, Ascenção CFR, Mellor C, Wang M, Smolka MB, Cohen PE.or with 10–25 μl (rabbit)HA antibody covalently bound to magnetic beads (Cell Signaling Technology 11846S). Lysate, bead, and antibody mixtures were incubated overnight at 4°C on a nutator. Samples were then

(14) Proteolytic activation of fatty acid synthase signals pan-stress resolutionNature MetabolismJanuary 2, 2024Hai Wei, Yi M. Weaver, Chendong Yang, Yuan Zhang, Guoli Hu, Courtney M. Karner, Matthew Sieber, Ralph J. DeBerardinis, Benjamin P. Weaverassay. IP was performed using HA tag (C29F4) rabbit monoclonal antibody (Cell Signaling Technology, 11846S) at 1:200 dilution for 4 h at 4 °C. Western blot was performed using mouse monoclonal antibody (Cell Signaling Technology

(15) Palmitoylation of PKCδ by ZDHHC5 in hypothalamic microglia presents as a therapeutic target for fatty liver diseaseTheranosticsJanuary 1, 2024Yan-Hang Wang, Xin Chen, Yi-Zhen Bai, Peng Gao, Zhuo Yang, Qiang Guo, Ying-Yuan Lu, Jiao Zheng, Dan Liu, Jun Yang, et al.(#90039), p-PKCδ (#9374), Histone H3 (#4499), Histone H3 (acetyl K9, #9649), Histone H4 (#13919), HA tag (#11846), SCD1 (#2794), β-actin (#3700), rabbit IgG (#7074) and mouse IgG (#7076) were purchased from Cell Signaling Technology

(16) Phosphatase and Pseudo-Phosphatase Functions of Phosphatase of Regenerating Liver 3 (PRL-3) Are Insensitive to Divalent Metals In VitroACS omegaAugust 22, 2023Jolly JT, Cheatham TC, Blackburn JS.Assay Purified HA-CBS was incubated with HA-Tag (C29F4) rabbit mAb magnetic bead conjugate (Cell Signaling, 11846S, Lot #9) at a concentration of 2.5 μM for 1 hour at room temperature suspended in immunoprecipitation

(17) Modulating p38 MAPK signaling by proteostasis mechanisms supports tissue integrity during growth and agingNature CommunicationsJuly 28, 2023Wang Yuan, Yi M. Weaver, Svetlana Earnest, Clinton A. Taylor, Melanie H. Cobb, Benjamin P. WeaverPMK-1 were used for immunoprecipitation with anti-HA-Tag (C29F4) rabbit mAb (Cell Signaling Technology 11846) and eluted with 2× laemmli buffer. Eluted protein was resolved by SDS-PAGE and gel slices of PMK-1

(18) A Novel Role of PP2A Methylation in the Regulation of Tight Junction Assembly and IntegrityFrontiers in Cell and Developmental BiologyJuly 13, 2022Diana Schuhmacher, Jean-Marie Sontag, Estelle Sontagfraction immunoprecipitated overnight at 4°C with either anti-HA (clone C29F4; Cell Signaling Technology #11846), or -myc (clone 9B11; Cell Signaling Technology #5698S) antibody coupled magnetic beads. Magnetic beads

(19) NOS2 and S -nitrosothiol signaling induces DNA hypomethylation and LINE-1 retrotransposon expressionProceedings of the National Academy of SciencesMay 24, 2022Christopher H. Switzer, Hyun-Ju Cho, Thomas R. Eykyn, Paul Lavender, Philip Eatonsupernatants were incubated overnight with anti–HA-tag–conjugated magnetic beads (Cell Signaling Technology, 11846) at 4 °C. Beads were then isolated in a magnetic field and washed three times with acetyltransferase

(20) Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesisCell reportsDecember 28, 2021Méndez-Solís O, Bendjennat M, Naipauer J, Theodoridis PR, Ho JJD, Verdun RE, Hare JM, Cesarman E, Lee S, Mesri EA.Conjugate,Cell Signaling,Cat.# 8726 HA-Tag Rabbit mAb Magnetic Bead Conjugate,Cell Signaling,Cat.# 11846 Critical commercial assays,, BCA protein assay,ThermoFisher Scientific,Cat.# 23227 VivaFix 410/450,BioRad,Cat.#

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HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) from Cell Signaling Technology

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