MHC Class II RT1B Antibody (OX-6) - BSA Free NB100-65541 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemMHC Class II RT1B Antibody (OX-6) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberNB100-65541
Quantity0.25 mg (also 0.05 mg)
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry Free-Floating,CyTOF-ready,Immunocytochemistry/ Immunofluorescence
Concentration1.0 mg/ml
FormatPBS
Conjugate/TagUnconjugated
PurityProtein G purified
CloneOX-6
TargetMHC Class II RT1B
IsotypeIgG1
ReactivityMouse,Rat
HostMouse
Antibody TypePrimary Antibodies
ImmunogenRat thymocyte membrane glycoproteins
ClonalityMonoclonal
Research AreaAdaptive Immunity, Immunology
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene NameRT1 class II, locus B

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunohistochemistry in rat models?

Yes, the MHC Class II RT1B Antibody (OX-6) has been utilized in immunohistochemistry applications, as demonstrated in studies involving rat models of Parkinson's disease, where it was used to assess the expression of MHC II in various tissues.

What specific cell types does this antibody target in rat tissues?

This antibody recognizes a monomorphic determinant of the rat I-A antigen, which is present on B lymphocytes, dendritic cells, some macrophages, and certain epithelial cells, making it suitable for studies involving these immune cell types.

Is there any evidence of this antibody being used in studies related to neuroinflammation?

Yes, the MHC Class II RT1B Antibody (OX-6) has been cited in research examining the role of the gut-brain axis in neuroinflammation, particularly in the context of Parkinson's disease, where it was used to evaluate the expression of MHC II in relation to inflammatory responses in the brain and gut.

What is the recommended storage condition for this antibody to maintain its stability?

The antibody should be stored at 4°C for short-term use and aliquoted for long-term storage at -20°C, avoiding freeze-thaw cycles to preserve its functionality.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Probing Gut Participation in Parkinson's Disease Pathology and Treatment via Stem Cell TherapyInternational journal of molecular sciencesJune 25, 2023Lee JY, Castelli V, Sanberg PR, Borlongan CV.Millipore, Burlington, MA, USA), α-synuclein (1:250, NBP2-15365; NOVUS, Centennial, CO, USA), MHC II (OX-6; NB100-65541; Novus Biologicals, Centennial, CO, USA) and TNF-α (ab6671; Abcam, Cambridge, MA, USA) was captured

(2) Inflammatory gut as a pathologic and therapeutic target in Parkinson's diseaseCell death discoverySeptember 24, 2022Lee JY, Wang ZJ, Moscatello A, Kingsbury C, Cozene B, Farooq J, Saft M, Sadanandan N, Gonzales-Portillo B, Zhang H, et al.X‐100. Sections were then incubated overnight at 4 °C with mouse monoclonal MHC II (anti‐RT1B (OX‐6); NB100‐65541; Novus Biologicals, Centennial, CO) or rabbit polyclonal TNF‐α (ab6671; Abcam, Cambridge, MA) antibodies

(3) Inflammation-relevant microbiome signature of the stroke brain, gut, spleen, and thymus and the impact of exerciseJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismDecember 1, 2021Kingsbury C, Shear A, Heyck M, Sadanandan N, Zhang H, Gonzales-Portillo B, Cozene B, Sheyner M, Navarro-Torres L, García-Sánchez J, et al.

(4) A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and NeurotoxicityStem cell reviews and reportsOctober 1, 2019Lee JY, Tuazon JP, Corey S, Bonsack B, Acosta S, Ehrhart J, Sanberg PR, Borlongan CV.(1:4000 tyrosine hydroxylase; 22941; Immunostar, Hudson, WI), mouse anti-rat MHC II (anti-RT1B (OX-6); NB100–65541; Novus Biologicals, Centennial, CO), or rabbit anti-rat TNF-α (ab6671; Abcam; Cambridge, MA) antibodies in

(5) Gutting the brain of inflammation: A key role of gut microbiome in human umbilical cord blood plasma therapy in Parkinson's disease modelJournal of cellular and molecular medicineAugust 1, 2019Lee JY, Tuazon JP, Ehrhart J, Sanberg PR, Borlongan CV.X‐100. Sections were then incubated overnight at 4°C with mouse anti‐rat MHC II (anti‐RT1B (OX‐6); NB100‐65541; Novus Biologicals, Centennial, CO) or rabbit anti‐rat TNF‐α (ab6671; Abcam, Cambridge, MA) antibodies

(6) Beneficial effects of liraglutide (GLP1 analog) in the hippocampal inflammationMetabolic brain diseaseOctober 1, 2017Barreto-Vianna ARC, Aguila MB, Mandarim-de-Lacerda CA.

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(9) Prenatal lipopolysaccharide does not accelerate progressive dopamine neuron loss in the rat as a result of normal agingExperimental neurologyApril 1, 2009Ling Z, Zhu Y, Tong CW, Snyder JA, Lipton JW, Carvey PM.

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(11) Progressive dopamine neuron loss following supra-nigral lipopolysaccharide (LPS) infusion into rats exposed to LPS prenatallyExperimental neurologyJune 1, 2006Ling Z, Zhu Y, Tong Cw, Snyder JA, Lipton JW, Carvey PM.

(12) 6-Hydroxydopamine-induced alterations in blood-brain barrier permeabilityThe European journal of neuroscienceSeptember 1, 2005Carvey PM, Zhao CH, Hendey B, Lum H, Trachtenberg J, Desai BS, Snyder J, Zhu YG, Ling ZD.

(13) Rotenone potentiates dopamine neuron loss in animals exposed to lipopolysaccharide prenatallyExperimental neurologyDecember 1, 2004Ling Z, Chang QA, Tong CW, Leurgans SE, Lipton JW, Carvey PM.

(14) Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunityThe Journal of experimental medicineDecember 7, 1998Stumbles PA, Thomas JA, Pimm CL, Lee PT, Venaille TJ, Proksch S, Holt PG.

(15) Functional activity of CD44 isoforms in haemopoiesis of the ratBritish journal of haematologyJanuary 1, 1997Khaldoyanidi S, Schnabel D, Föhr N, Zöller M.

(16) Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in ratsJournal of virologyJune 1, 1992Stitz L, Sobbe M, Bilzer T.

(17) CD4 rat x rat and mouse x rat T cell hybridomas produced by fusion of established T cell lines and clones to W/Fu (C58NT)DJournal of immunological methodsNovember 5, 1991Boots AM, van Lierop MJ, Wauben MH, van Kooten PJ, Hensen EJ, van Eden W.

(18) Borna disease virus-induced meningoencephalomyelitis caused by a virus-specific CD4+ T cell-mediated immune reactionThe Journal of general virologyNovember 1, 1990Richt J, Stitz L, Deschl U, Frese K, Rott R.

(19) T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implicationsJournal of virologyAugust 1, 1990Lazo PA, Klein-Szanto AJ, Tsichlis PN.

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