Immunofluorescence analysis of HeLa cells using CD95/Fas monoclonal antibody (Product # MA5-17073) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Western Blot was performed using Anti-FAS Monoclonal Antibody (4F8H6) (Product # MA5-17073) and a 16 kDa band corresponding to an isoform of FAS (Tumor necrosis factor receptor superfamily member 6) was observed across cell lines tested except in HUVEC and MDA-MB-231 which are reported low expressing for the protein. Whole cell extracts (40 µg lysate) of Jurkat (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), HUVEC (Lane 4), MDA-MB-231 (Lane 5), NK-92 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).