Flow Cytometry: PIEZO1 Antibody [TA309652] - An intracellular stain was performed on MCF7 cells with PIEZO1 Antibody TA309652 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunohistochemistry: PIEZO1 Antibody [TA309652] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.