Immunocytochemistry/Immunofluorescence: CENPF Antibody [TA309605] - CENPF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunocytochemistry/Immunofluorescence: CENPF Antibody [TA309605] - Confocal immunofluorescent analysis of HeLa cells using CENPF antibody (TA309605, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

Western Blot: CENPF Antibody [TA309605] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.