Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [TA309804] - Analysis of PKM2 in HeLa cells.

Simple Western: PKM2 Antibody - BSA Free [TA309804] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Flow Cytometry: PKM2 Antibody - BSA Free [TA309804] - An intracellular stain was performed on HeLa cells with PKM2 Antibody TA309804AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.