Fig 1: TRPS1 Chromatin Binding Partners and Their Changes after TRPS1 Knockdown(A) Word cloud depicting the top 50 TRPS1-associated proteins identified by RIME in HCC3153 and MDA-IBC3 cell lines. Font size reflects the ratio of peptide count in anti-TRPS1 versus control IgG immunoprecipitation. Colors indicate NuRD (red) and switch/sucrose non-fermentable (SWI/SNF) (blue) complex proteins. TRPS1 signal (gray) is represented in the background.(B) Top 10 pathways enriched forTRPS1-interacting proteins in both HCC3153 and MDA-IBC3 cell lines.(C) Heatmap of TRPS1 (group 1), GATAD2A (group 2), and GATAD2B (group 3)-unique, TRPS1 and GATAD2A (group 4), GATAD2A and GATAD2B (group 5), TRPS1 and GATAD2B (group 6), and common for all 3 marks (group 7) peaks in HCC3153 cells. Numbers of peaks in each group are indicated.(D) Integrated analysis of gene expression changes after TRPS1 knockdown and TRPS1 chromatin occupancy for chromatin regions bound by TRPS1 only or TRPS1 and GATAD2B.(E) Top 10 upregulated and downregulated process networks for genes differentially expressed after TRPS1 knockdown and associated with TRPS1-GATAD2B-overlapping peaks in HCC3153 cells.(F) Examples of TRPS1 and GATAD2B-overlapping peaks.(G) Heat-map of GATAD2A and GATAD2B peaks in HCC3153 cells before and after TRPS1 knockdown. Each group represents peaks for TRPS1 (group 1), GATAD2A (group 2), GATAD2B (group 3), TRPS1 and GATAD2A (group 4), GATAD2A and GATAD2B (group 5), TRPS1 and GATAD2B (group 6), common for all 3 marks (group 7).See also Figure S5.