Topoisomerase IIalpha (D10G9) XP ® Rabbit mAb 12286S from Cell Signaling Technology

Product Specs
ItemTopoisomerase IIalpha (D10G9) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $402.00 Inquire
Catalog Number12286S
Quantity100 µl
ApplicationsW, IHC-P, IF-IC, FC-FP
ReactivityH, Mk
TargetTOP2A
HostRabbit
NCBI Full Gene NameDNA topoisomerase II alpha
NCBI Gene AliasesTOP2, TOP2alpha, TOPIIA, TP2A
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
CloneD10G9
Molecular Weight190

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) HNF1B-MODY in the Norwegian MODY Registry and the Norwegian Childhood Diabetes Registry: Clinical Insights and Prevalence Informed by Genetic and Functional EvaluationInt J Mol SciJune 3, 2026Aishwarya Pavithram, Bente B. Johansson, Erling Tjora, Pernille Svalastoga, Khadra A. Mohamed, Ingvild L. Koløen, Maren Toftdahl, Torild Skrivarhaug, Marc Vaudel, Lise Bjørkhaug, et al.topoisomerase I (Abcam #ab196642, Cambridge, UK) and topoisomerase II alpha (Cell Signaling Technology #12286S, Danvers, MA, USA) served as nuclear markers for HeLa and MIN6 cells, respectively, and alpha-tubulin

(2) Cell confinement initiates a delayed but heritable loss of chromosomesCell RepMarch 24, 2026Steven H. Phan, Mai Wang, Kayla R. Snare, Jonah Kandell, Jeneille S. Deans, Panteleimon Rompolas, Guilherme P.F. Nader, Dennis E. Discher(Thermo Fisher Scientific 62249) for 15 min. The following primary antibodies were used: TOP2A (Cell Signaling Technology 12286), LMNA/C (Cell Signaling Technology 4777), Tubulin (Cell Signaling Technology 2148). The following secondary

(3) Overcoming acquired doxorubicin resistance of ovarian carcinoma cells by verapamil‑mediated promotion of DNA damage‑driven cytotoxicityInternational Journal of OncologyFebruary 24, 2026Elvira Mukinovic, Sina Federmann, Larissa Messling, Marlena Sekeres, Julia Mann, Lena Abbey, Matthias U. Kassack, Gerhard FritzTopBP1 (D8G4L; cat. no. 14342, Cell Signaling Technology, Inc.), Topoisomerase II alpha (D10G9; cat. no. 12286, Cell Signaling Technology, Inc.). After washing (TBS/0.1% Tween 20), the secondary (peroxidase-conjugated)

(4) Aberrant CDK4/6-driven cell-cycle reentry drives neuronal loss and defines a therapeutic target in C9orf72 ALS/FTDiScienceFebruary 20, 2026Ling Lian, Hayley Robinson, Noah Daniels, G. Aleph Prieto, Gunnar H.D. Poplawski, Rodrigo Lopez-Gonzalezanti-Phospho-Rb (Ser807/811),Cell Signaling,Cat# 9308; RRID:AB_331472 Rabbit anti-Topoisomerase IIα,Cell Signaling,Cat# 12286; RRID:AB_2797871 Rabbit anti-C9orf72,Cell Signaling,Cat# 64196; RRID:AB_2833080 Rabbit anti-PUMA (BBC3),Cell Signaling

(5) Cotargeting DNA topoisomerase II enhances efficacy of RAS-targeted therapy in KRAS-mutant cancer modelsJ Clin InvestFebruary 16, 2026Rongzhong Xu, Dongsheng Wang, Guangzhi Ma, Xun Yuan, Qian Chu, Songqing Fan, Rener Zhang, Du, Shidong Jia, Ticiana A. Leal, et al.patients. Reagents. Sotorasib (AMG510), adagrasib, VP-16, and DXR were purchased from MedChemExpress. Topo IIα (catalog 12286) and Bim (catalog 2933) antibodies were purchased from Cell Signaling Technology. Mcl-1 antibody (sc-12756)

(6) Cotargeting DNA topoisomerase II enhances efficacy of RAS-targeted therapy in KRAS-mutant cancer modelsJ Clin InvestFebruary 16, 2026Rongzhong Xu, Dongsheng Wang, Guangzhi Ma, Xun Yuan, Qian Chu, Songqing Fan, Rener Zhang, Pan Du, Shidong Jia, Ticiana A. Leal, et al.patients. Reagents. Sotorasib (AMG510), adagrasib, VP-16, and DXR were purchased from MedChemExpress. Topo IIα (catalog 12286) and Bim (catalog 2933) antibodies were purchased from Cell Signaling Technology. Mcl-1 antibody (sc-12756)

(7) Cell confinement initiates a delayed but heritable loss of chromosomesbioRxivFebruary 3, 2026Steven H. Phan, Mai Wang, Kayla R. Snare, Jonah Kandell, Jeneille S. Deans, Panteleimon Rompolas, Guilherme P.F. Nader, Dennis E. Discher(Thermo Fisher Scientific 62249) for 15 min. The following primary antibodies were used: TOP2A (Cell Signaling Technology 12286), LMNA/C (Cell Signaling Technology 4777), Tubulin (Cell Signaling Technology 2148). The following secondary

(8) SRSF3 knockdown-induced cellular senescence as a possible therapeutic strategy for non-small cell lung cancerCarcinogenesisNovember 21, 2025Shinji Nakamichi, Natalia von Muhlinen, Leo Yamada, Jilian R. Melamed, Tyler E. Papp, Hamideh Parhiz, Drew Weissman, Izumi Horikawa, Curtis C. HarrisBurlington, MA); UBE2C (Santa Cruz Biotechnology, sc-271050); TOP2A (Cell Signaling Technology, no. 12286); GAPDH (EMD Millipore, MAB374); and β-actin (Abcam, ab6276, Waltham, MA). Secondary antibodies used

(9) Transcriptional analysis reveals a markedly reduced expression of the voltage-dependent calcium channel α2δ1 subunit in canine prostate cancer compared to benign prostatic hyperplasiaBMC Vet ResOctober 9, 2025Maciej Zacharski, Maciej Ugorski, Przemysław Prządka, Stanislaw Dzimira, Sonja Škevin, Iwona Sidorkiewicz, Bartłomiej Liszka, Piotr Skrzypczak, Filip Van Nieuwerburgh, Adam Krętowski, et al.channel subunit α2δ1, Thermo-Fisher, cat.no MA3-921; rabbit monoclonal antibody anti-TOP2A, CST, cat. no. 12286 T; rabbit polyclonal antibody anti-GOLGA4, CST, cat. no. 79145 S; rabbit polyclonal antibodies anti-CLDN10,

(10) Decoding functional and developmental trajectories of tissue-resident uterine dendritic cells through integrative omicsiScienceSeptember 19, 2025Aditi Singh, Jing Yang, Nicholas K. Adzibolosu, Songchen Cai, Elana Kauf, Lingtao Yang, Qiyuan Li, Hanjie Li, Alexandra Werner, Siddharth Parthasarathy, et al.Anti-LYZ (Sino Biology, 110207-M095), Anti-CLEC10A (abcam, ab315086), Anti-Topoisomerase IIα (CST, 12286S), Anti-CD14 (Sino Biology, 10073-R001), Anti-IRF-8 (CST, 83413S), Anti-XCR1 (CST, 44665S), Anti-Granzyme

(11) Molecular Mechanism Analysis of the Effect of Hederagenin Combined with L-OHP on Chemosensitivity of AGS/L-OHP based on Network PharmacologyCurrent Computer-Aided Drug DesignAugust 1, 2025Hongyue Tang, Chao Wang, Chenhao Xing, Guoxin liang, Chang Guo, Xin Liu, YanJie Li, Mingming Zhangfrom Cell Signaling Technology, USA. Rabbit-anti-human MDR1/P-gp(#13342) Survivin(#2808), TOPOIIα (#12286), and GAPDH(#5174) were purchased from Cell Signaling Technology, and mouse-anti-human antibody Bcl-2(sc-7382)

(12) Targeting Artemis Sensitizes B Cells to Topoisomerase 2 Poisons by Disrupting DNA-PKcs-Dependent RepairResearch SquareJune 20, 2025Melissa L. Pannunzio, Melissa Folkerts, Cameron Nguyen, Kaiyuan V. Nguyen, Kaiyuan Rangel, Valeria Rangel, Pedro Buisson, Selma Buisson, Noritaka Adachi, Noritaka Adachi, et al.with TBST and incubated overnight at 4°C with a 1:1000 dilution in BLOTTO of Top2A (Cell signaling; 12286). Membranes were then washed 3 times for 5min with 1x TBST and incubated at room temperature with a

(13) Analysis of tumor cell proliferation (Ki-67) and cell cycle regulator proteins in lung adenocarcinoma with different radiological subtypesRespir ResApril 13, 2025Rirong Qu, Yang Zhang, Shenghui Qin, Jing Xiong, Xiangning Fu, Lequn Li, Dehao Tu, Yixin CaiUSA), Cyclin A2 (CCNA2) (clone# 67955, 1:1600 dilution; Cell Signaling Technology, MA, USA), TOP2A (12286 T, 1:400 dilution; Cell Signaling Technology, MA, USA), pRb (clone# 8516 T, 1:800 dilution; Cell Signaling Technology

(14) Models of Early Resistance to CDK4/6 Inhibitors Unveil Potential Therapeutic Treatment SequencingInt J Mol SciMarch 14, 2025Elisabet Zapatero-Solana, Yan Ding, Nicholas Pulliam, Alfonso de Dios, Maria Jesus Ortiz-Ruiz, María José LallenaCell Signaling), FoxM1 (180710, Abcam), p21 (2947, Cell Signaling), p27 (3686, Cell Signaling), Topoisomerase IIα (12286, Cell Signaling), Mcl-1 (5453, Cell Signaling), Phospho-cdc2 (Tyr15; 9111, Cell Signaling), Wee1 (13084,

(15) SUMOylation of protein phosphatase 5 regulates phosphatase activity and substrate releaseEMBO reportsNovember 1, 2024Sager RA, Backe SJ, Dunn DM, Heritz JA, Ahanin E, Dushukyan N, Panaretou B, Bratslavsky G, Woodford MR, Bourboulia D, et al.anti-phos-Ser1213-TOP2A,Invitrogen,Cat# PA5-105828; RRID:AB_2817227 Rabbit anti-TOP2A (D10G9),Cell Signaling Technology,Cat# 12286; RRID:AB_2797871 Goat anti-FNIP1,Antibodies-online.com,Cat# ABIN238670; RRID:AB_10775640 Anti-mouse

(16) DNA topoisomerase II inhibition potentiates osimertinib’s therapeutic efficacy in EGFR-mutant non–small cell lung cancer modelsJournal of Clinical InvestigationMay 15, 2024Zhen Chen, Karin A. Vallega, Dongsheng Wang, Zihan Quan, Songqing Fan, Qiming Wang, Ticiana Leal, Suresh S. Ramalingam, Shi-Yong Suncyclophosphamide, capecitabine, and vincristine were purchased from MedChemExpress. Topo IIα (catalog 12286), Smurf2 (catalog 12024), cPARP (catalog 5625), and Bim (catalog 2933) antibodies were purchased from

(17) Functional characterization of HNF4A gene variants identify promoter and cell line specific transactivation effectsHuman Molecular GeneticsMay 4, 2024Alba Kaci, Marie Holm Solheim, Trine Silgjerd, Jorunn Hjaltadottir, Lorentze Hope Hornnes, Janne Molnes, Andre Madsen, Gry Sjøholt, Christine Bellanné-Chantelot, Richard Caswell, et al.was verified using antibodies against the nuclear marker protein Topoisomerase II-α (Cell signaling; #12286) and the cytosolic marker protein GAPDH (Santa Cruz; sc-47724). The relative subcellular localization

(18) DNA topoisomerase II inhibition potentiates osimertinib’s therapeutic efficacy in EGFR-mutant non–small cell lung cancer modelsJ Clin InvestMarch 7, 2024Zhen Chen, Karin A. Vallega, Dongsheng Wang, Zihan Quan, Songqing Fan, Qiming Wang, Ticiana Leal, Suresh S. Ramalingam, Shi-Yong Suncyclophosphamide, capecitabine, and vincristine were purchased from MedChemExpress. Topo IIα (catalog 12286), Smurf2 (catalog 12024), cPARP (catalog 5625), and Bim (catalog 2933) antibodies were purchased from

(19) MDM2 provides TOP2 poison resistance by promoting proteolysis of TOP2βcc in a p53-independent mannerCell Death & DiseaseJanuary 23, 2024Jianfeng Shu, Jinni Jiang, Xiaofang Wang, Xuejie Yang, Guofang Zhao, Ting CaiCell Signaling Technology, 1:500), TOP2β (611493, BD Biosciences, 1:1000), TOP2β (sc-25330, Santa Cruz, 1:1000), TOP2α (12286, Cell Signaling Technology, 1:1000), p53 (2527 S, Cell Signaling Technology, 1:1000), p21 (2947 S, Cell Signaling Technology

(20) Rescue of p53 functions by in vitro‐transcribed mRNA impedes the growth of high‐grade serous ovarian cancerCancer CommunicationsJanuary 1, 2024Monika Raab, Izabela Kostova, Samuel Peña‐Llopis, Daniela Fietz, Monika Kressin, Seyed Mohsen Aberoumandi, Evelyn Ullrich, Sven Becker, Mourad Sanhaji, Klaus StrebhardtCell Signaling), Noxa (#14766) (1:1000; Cell Signaling), Fas (#8023) (1:1000; Cell Signaling), TOP2A (#12286) (1:1000; Cell Signaling), BIRC5 (#2802) (1:1000; Cell Signaling), Nup98 (#2598) (1:1000; Cell Signaling),

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Topoisomerase IIalpha (D10G9) XP ® Rabbit mAb

Topoisomerase IIalpha (D10G9) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Topoisomerase IIalpha (D10G9) XP ® Rabbit mAb from Cell Signaling Technology

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