ELAVL1/HuR (D9W7E) Rabbit mAb 12582S from Cell Signaling Technology

Product Specs
ItemELAVL1/HuR (D9W7E) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12582S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP
ReactivityH, M, R, Mk
TargetELAVL1
HostRabbit
NCBI Full Gene NameELAV like RNA binding protein 1
NCBI Gene AliasesELAV1, HUR, Hua, MelG
ClonalityMonoclonal
Research AreaTranslational Control
CloneD9W7E
Molecular Weight30

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Cell Signaling Technology

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(1) RNA-binding protein ELAVL1 modulates WDR36 to inhibit p53 pathway and reduce calcium overload in retinal cells under acute pressure elevationOpen Life SciApril 20, 2026QingFeng Meng, BaoLi Ning, WenXiong Chen, JingJing Zhang, Jun FuScientific) and analyzed with ImageJ software (version 1.50b). Primary antibodies used were: ELAVL1 (#12582, Cell Signaling Technology), p53 (#2527, Cell Signaling Technology), phospho-p53 (Ser15) (#9284, Cell Signaling Technology

(2) Druglike Molecular Degraders of the Oncogenic RNA-Binding Protein HuRJACS AuJuly 16, 2025Liann Kassabri, Raphael I. Benhamou°C with primary antibodies: a 1:1000 dilution of rabbit anti-ELAVL1/HuR (Cell Signaling Technology, #12582), a 1:1000 dilution of mouse anti-Bcl2 (Cell Signaling Technology, #15071), or a 1:10,000 dilution of

(3) Ribonucleotide reductase small subunit M2 promotes the proliferation of esophageal squamous cell carcinoma cells via HuR-mediated mRNA stabilizationActa Pharmaceutica Sinica BOctober 1, 2024Jing Zhang, Qiong Wu, Yifei Xie, Feng Li, Huifang Wei, Yanan Jiang, Yan Qiao, Yinhua Li, Yanan Sun, Han Huang, et al.against RRM2 (Abcam, Cambridge, UK, ab57653), HuR (Cell Signaling Technology, Danvers, MA, USA, cat# 12582), Actin (ZSGB-BIO, Beijing, China, cat# TA-09), GAPDH (ZSGB-BIO, Beijing, China, cat# TA-08) and Flag

(4) Lipolysis-Stimulated Lipoprotein Receptor in Proximal Tubule, BMP-SMAD Signaling, and Kidney DiseaseJournal of the American Society of NephrologyAugust 1, 2024Min Jiang, Xiangdong Wang, Zhenni Chen, Xin Wang, Yanan An, Lixia Ding, Mengyuan Xu, Baozhen Fan, Peng Jiao, Chao Wang, et al.epithelial cell lysates were immunoprecipitated with human antigen R antibody (3 μg, Cell Signaling Technology, 12582S) or control IgG (3 μg, Invitrogen, C10400C) for 1 hour. RNA and protein were extracted from immobilized

(5) Pro-survival signaling regulates lipophagy essential for multiple myeloma resistance to stress-induced deathCell ReportsJuly 23, 2024Peng Peng, Colin Chavel, Wensheng Liu, Louise M. Carlson, Sha Cao, Adam Utley, Scott H. Olejniczak, Kelvin P. Lee,Cell Signaling,Cat#5869; RRID:AB_10707365 ATG5,Cell Signaling,Cat#12994; RRID:AB_2630393 ELAVL1/HuR,Cell Signaling,Cat#12582; RRID:AB_2797964 CPT1A,Cell Signaling,Cat#97361; RRID:AB_2895298 Ubiquitin,Cell Signaling,Cat#3936;

(6) HuR controls glutaminase RNA metabolismNature CommunicationsJuly 4, 2024Douglas Adamoski, Larissa M. dos Reis, Ana Carolina Paschoalini Mafra, Felipe Corrêa-da-Silva, Pedro Manoel Mendes de Moraes-Vieira, Ioana Berindan-Neagoe, George A. Calin, Sandra Martha Gomes Dias(RheaBiotech IM-0322), KGA (RheaBiotech IM-0519), HuR (Molecular Probes mp21277 and Cell Signaling 12582), and V5 (Life Technologies 46-1157). pLKO.1 puro was a gift from Bob Weinberg (Addgene plasmid # 8453);

(7) HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formationBone ResearchOctober 23, 2023Yuheng Li, Weijia Sun, Jianwei Li, Ruikai Du, Wenjuan Xing, Xinxin Yuan, Guohui Zhong, Dingsheng Zhao, Zizhong Liu, Xiaoyan Jin, et al.Cat. No. ab420, monoclonal) or rabbit anti-HuR antibody (1:300, Cell Signaling Technology, Cat. No. 12582) in 1× blocking buffer at room temperature for 1 h, washed 3 times with TBST for 5 min, incubated with

(8) The kinesin light chain‐2, a target of mRNA stabilizing protein HuR, inhibits p53 protein phosphorylation to promote radioresistance in NSCLCThoracic CancerJune 1, 2023Simiao Qiao, Yuhang Jiang, Na Li, Xiaoxia ZhuRNase inhibitor cocktail. The antibody used in this assay was ELAVL1 (Cell Signaling Technology, no. 12582S). Magnetic beads were preincubated with an anti‐flag antibody or anti‐rabbit IgG for 30 min at room

(9) Optimization of Biotinylated RNA or DNA Pull-Down Assays for Detection of Binding Proteins: Examples of IRP1, IRP2, HuR, AUF1, and Nrf2International journal of molecular sciencesFebruary 10, 2023Tsuji Y.anti-IRP2,Cell Signaling Technology,37135,1 rabbit monoclonal anti-HuRELAVL1,Cell Signaling Technology,12582,1 rabbit monoclonal anti-AUF1/hnRNP D,Cell Signaling Technology,12382,1 rabbit polyclonal anti-Nrf2,Santa Cruz Biotechnology

(10) NF-κB1 p50 stabilizes HIF-1α protein through suppression of ATG7-dependent autophagyCell death & diseaseDecember 27, 2022Zhu J, Huang S, Li Y, Xu J, Chen R, Guo M, Qian X, Li T, Tian Z, Jin H, et al.Beclin1, LC3A, LC3B (Autophagy Antibody Sampler Kit #4445), ATG16L1 (#8089), P62 (#8025), ELAVL1 (#12582), Elk1 (#9182), Ets1 (#14069), STAT5 (#25656), and GAPDH (#5174) were bought from Cell Signaling Technology

(11) ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16(INK4a) levelsNature cell biologyAugust 1, 2022Hao X, Shiromoto Y, Sakurai M, Towers M, Zhang Q, Wu S, Havas A, Wang L, Berger S, Adams PD, et al.anti-SIRT1 (Millipore, cat. no. 07-131; 1:1000), rabbit anti-HuR (Cell Signaling Technology, cat. no. 12582; 1:1000), mouse anti-p16INK4a (Abcam, cat. no. ab16123, 1:500), mouse anti-p21 (Abcam, cat. no. 7901;

(12) Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear exportCell reportsJuly 19, 2022Duan L, Zaepfel BL, Aksenova V, Dasso M, Rothstein JD, Kalab P, Hayes LR.ab129160; RRID:AB_11142853 Rabbit anti-ELAVL1/HuR monoclonal (IF: 1:100),Cell Signaling Technology,Cat# 12582; RRID:AB_2797964 Mouse anti-DEK monoclonal (IF: 1:100),Santa Cruz Biotechnology,Cat# sc-136222; RRID:AB_2245948 Mouse

(13) Autophagy-associated circRNA circATG7 facilitates autophagy and promotes pancreatic cancer progressionCell death & diseaseMarch 14, 2022He Z, Cai K, Zeng Z, Lei S, Cao W, Li X.Cell Signaling Technology, Inc.), P62 (1:1000; Cat No. 18420-1-AP, Proteintech Group), LC3 (1:1000), HUR (1:500; Cat No. 12582, Cell Signaling Technology, Inc.), and GAPDH (1:500; Cat No.10494-1-AP, Proteintech Group) primary antibodies

(14) Msi2-mediated MiR7a-1 processing repression promotes myogenesisJournal of cachexia, sarcopenia and muscleFebruary 1, 2022Yang W, Yang L, Wang J, Zhang Y, Li S, Yin Q, Suo J, Ma R, Ye Y, Cheng H, et al.(Millipore, 04‐1069), Mouse anti‐Msi2 (Abnova, H00124540‐M11), Rabbit anti‐HuR (Cell Signaling Technology, 12582), Mouse anti‐HuR (Santa Cruz, sc‐5261), Mouse anti‐Flag tag (Abmart, ab1162), Rabbit anti‐Myc tag (Proteintech,

(15) TCF4 and HuR mediated-METTL14 suppresses dissemination of colorectal cancer via N6-methyladenosine-dependent silencing of ARRDC4Cell death & diseaseDecember 17, 2021Wang H, Wei W, Zhang ZY, Liu Y, Shi B, Zhong W, Zhang HS, Fang X, Sun CL, Wang JB, et al.Thermo Fisher Scientific). Primary antibodies were listed as: anti-METTL14 (HPA038002, Sigma-Aldrich), anti-HuR (#12582, CST), anti-IGF2BP1 (22803-1-AP, Proteintech), anti-YTHDC1 (14392-1-AP, Proteintech), anti-YTHDF1 (17479-1-AP,

(16) Long Noncoding RNA SNHG12 Promotes Gastric Cancer Proliferation by Binding to HuR and Stabilizing YWHAZ Expression Through the AKT/GSK-3β PathwayFrontiers in OncologyJune 14, 2021Tianqi Zhang, Maneesh Kumarsing Beeharry, Yanan Zheng, Zhenqiang Wang, Jianfang Li, Zhenggang Zhu, Chen Li(Cell Signaling Technology, #9315S), p-GSK-3β (ABclonal, #AP1088), and ELAVL1 (Cell Signaling Technology, #12582S) were used in this study. Cell Proliferation Detection For the Cell Counting Kit-8 (CCK-8) assay,

(17) The RNA-binding protein HuR is a novel target of Pirh2 E3 ubiquitin ligaseCell death & diseaseJune 5, 2021Daks A, Petukhov A, Fedorova O, Shuvalov O, Kizenko A, Tananykina E, Vasileva E, Semenov O, Bottrill A, Barlev N.Cell Signaling Technology, Beverly, MA, USA), FLAG (F1804, Sigma Aldrich, St. Louis, MO, USA), HuR (12582S, Cell Signaling Technology, Beverly, MA, USA), β-tubulin (T8535, Sigma Aldrich, St. Louis, MO, USA),

(18) Smooth muscle-specific HuR knockout induces defective autophagy and atherosclerosisCell death & diseaseApril 9, 2021Liu S, Jiang X, Cui X, Wang J, Liu S, Li H, Yang J, Zhang C, Zhang W.run on a 12% SDS-PAGE gel and immunoblotted overnight with the primary antibodies for HuR (29 ng/ml, 12582S, CST), AMPKα1 (1 μg/ml, Ab32047, Abcam), AMPKα2 (1 μg/ml, ab3760, Abcam), p-AMPK (27 ng/ml, 2535S, CST),

(19) HuR up-regulates cell surface PD-L1 via stabilizing CMTM6 transcript in cancerOncogeneMarch 1, 2021Liu Y, Li X, Zhang H, Zhang M, Wei Y.anti-PD-L1, UMAB228, Origene, MD, USA; rabbit anti-CMTM6, HPA026980, Sigma-Aldrich; rabbit anti-HuR, #12582; rabbit anti-β-actin, #4967; rabbit anti-GAPDH, #2118; Cell Signaling Technology) hybridization was

(20) Human antigen R regulates hypoxia-induced mitophagy in renal tubular cells through PARKIN/BNIP3L expressionsJournal of cellular and molecular medicineMarch 1, 2021Yu SH, Palanisamy K, Sun KT, Li X, Wang YM, Lin FY, Chen KB, Wang IK, Yu TM, Li CY.no: GTX111876, 1:1000, GeneTex), anti‐BNIP3 (Cat no: GTX10433, 1:1000, GeneTex), anti‐HuR (Cat no: #12582, 1:1000, Cell Signaling Technology), anti‐TOMM20 (Cat no: ab56783, 1:200, Abcam), anti‐LC3 (Cat no:

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ELAVL1/HuR (D9W7E) Rabbit mAb

ELAVL1/HuR (D9W7E) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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ELAVL1/HuR (D9W7E) Rabbit mAb from Cell Signaling Technology

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