Vitamin D3 Receptor (D2K6W) Rabbit mAb 12550S from Cell Signaling Technology

Product Specs
ItemVitamin D3 Receptor (D2K6W) Rabbit mAb
CompanyCell Signaling Technology
Price $357.00 Inquire
Catalog Number12550S
Quantity100 µl
ApplicationsW, IP, IHC-P, ChIP, ChIP-seq
ReactivityH, M
TargetVitamin D3 Receptor
Molecular Weight48, 54
HostRabbit
NCBI Full Gene Namevitamin D receptor
NCBI Gene AliasesNR1I1, PPP1R163
ClonalityMonoclonal
CloneD2K6W
Research AreaNuclear Receptor Signaling

Questions and Answers

What species does this antibody react with, and has it been used in relevant studies?

The Vitamin D3 Receptor (D2K6W) Rabbit mAb recognizes endogenous levels of total vitamin D3 receptor protein in both human and mouse species. It has been cited in 46 scientific articles, indicating its utility in various research contexts.

Can this antibody be used for ChIP assays, and what evidence supports its use?

Yes, this antibody has been shown to work in Chromatin Immunoprecipitation (ChIP) assays. For instance, in a study investigating gene regulation, the antibody was utilized to assess VDR recruitment to vitamin D response elements, demonstrating its effectiveness in this application ().

Is there any evidence of this antibody being used in cancer research?

Yes, the antibody has been employed in studies related to cancer, such as in the context of acute myeloid leukemia. Research has shown that activation of the vitamin D receptor pathway can enhance differentiation in cancer cells, indicating its potential role in therapeutic strategies ().

What applications has this antibody been validated for in scientific literature?

The Vitamin D3 Receptor (D2K6W) Rabbit mAb has been validated for use in Western blotting, immunohistochemistry, immunoprecipitation, and ChIP assays, as evidenced by its application in various studies exploring the role of vitamin D receptors in cellular processes and disease mechanisms.

Cell Signaling Technology

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United States

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Impaired vitamin D signaling reveals neutrophils as key drivers of prostate cancer disseminationEMBO Molecular MedicineApril 10, 2026Kateryna Len-Tayon, Justine Gantzer, Charles Dariane, Olivier Fogel, Vanessa Friedrich, Daniela Rovito, Véronique Lindner, Valentine Gilbart, Darya Yanushko, Sandrine Henri, et al.incubated overnight with one of the primary antibodies [rabbit anti-VDR (Cell Signaling Technology Cat# 12550, RRID:AB_2637002; 1:500 dilution), mouse anti-β-Actin (Sigma-Aldrich Cat# A1978, RRID:AB_476692; 1:1000

(2) Vitamin D deficiency alters prostate epithelial differentiation and increases prostate cancer aggressiveness in ex vivo and in vivo modelsbioRxivFebruary 20, 2026Adriana Duraki, Kirsten D. Krieger, Sasha Celada, Robert A. Holt, Ryan M. Brown, Luyu Wang, Michael J. Schlicht, Maarten C. Bosland, Robert M. Sargis, Donald Vander Griend, et al.room temperature and incubated overnight at 4° C with primary antibodies specific to VDR (D2K6W; CST 12550), AR (PG-21; EMD Millipore 06–680), FKBP5 (D5G2; CST 12210), and housekeeper H3 (96C10; CST 3638). All

(3) Vitamin D3 ameliorates R-loop-induced replication stress and chromosomal instability in MED12-mutant uterine fibroidsResearch SquareJanuary 14, 2026Sribalasubashini Muralimanoharan, Ana Corachán, Azad Khosh, Sierra Hathaway, Susivarshini Karthigayan, Claire Schenken, Nicholas Stansbury, Robert Schenken, Maria Victoria Bariani, Qiwei Yang, et al.MedChem Express) for 48h and immunoblot analysis was performed using antibodies specific for VDR (#12550; Cell Signaling) and GAPDH (#2118; Cell signaling) was used as the loading control. Immunofluorescent staining

(4) Vitamin D Regulates Olfactory Function via Dual Transcriptional and mTOR‐Dependent Translational Control of Synaptic ProteinsAdvanced ScienceDecember 16, 2025Pengcheng Ren, Renhe Cao, Xiaoshan Ye, Wenbin Pang, Qingshang Bi, Meihui Huang, Qionglin Zhou, Dan Ye, Wei Xiang, Le XiaoGermany). The following primary antibodies were used: rabbit anti‐VDR (1:500; Cell Signaling Technology, 12550S); mouse anti‐calbindin D‐28k (1:5000; Swant CB300PUR); mouse anti‐calretinin (1:5000; 6B3, Swant); guinea pig

(5) Interindividual Genetic Differences Drive Discordance Between Serum Calcidiol and Calcitriol Concentrations in FemalesEndocrinologySeptember 8, 2025Elizabeth K Hutchins, Changran Niu, Jing Xue, Debin Wan, Carolina V Campos, Molly Warren, Megan M Knuth, Michael B Whalen, Venkata S Voruganti, Rafiou Agoro, et al.hour at room temperature, then incubated with primary antibodies for VDR (D2K6W, Cell Signaling, Cat #12550, Antibody Registry ID: AB_2637002) and B-actin (Cell Signaling, Cat #4970S, Antibody Registry ID: AB_2223172)

(6) Co-treatments of vitamin D and mesenchymal stem cells effectively alleviate the diabetic kidney disease through attenuating the SIRT1-mediated pathwaysStem Cell Res TherAugust 26, 2025Xinfeng Zhao, Sirui He, Xin Luo, Xiaru Zhang, Xueyi Jiang, Yueqing Liang, Tiantian Tang, Kemin Qi, Yang Wang, Yi Zhang, et al.ALP (no. sc-365765, Santa Cruz, 1:100), Runx2 (no. 8486, Cell Signaling Technology, 1:1000), VDR (no. 12550, Cell Signaling Technology, 1:1000), CYP27B1 (no. sc-515903, Santa Cruz, 1:100), CYP24A1 (no. K002997P,

(7) Calcitriol and Tacalcitol Modulate Th17 Differentiation Through Osteopontin Receptors: Age-Dependent Insights from a Mouse Breast Cancer ModelImmunotargets TherAugust 23, 2025Aleksandra Strzykalska-Augustyniak, Mateusz Psurski, Honorata Zachary, Beata Filip-Psurska, Dagmara Kłopotowska, Magdalena Milczarek, Marta Świtalska, Martyna Stachowicz-Suhs, Natalia Łabędź, Aleksandra Ziemblicka, et al.9216S",CHEMI "Cell Signaling Technology, Danvers, MA, USA",Vitamin D3 Receptor (D2K6W),IgG,"1:125, catalog no: 12550S",CHEMI Antibodies Used for Immunodetection Using Jess Simple Western Method Notes: Near-infrared

(8) Calcitriol and Tacalcitol Modulate Th17 Differentiation Through Osteopontin Receptors: Age-Dependent Insights from a Mouse Breast Cancer ModelImmunotargets TherAugust 23, 2025Aleksandra Strzykalska-Augustyniak, Mateusz Psurski, Honorata Zachary, Beata Filip-Psurska, Dagmara Kłopotowska, Magdalena Milczarek, Marta Świtalska, Martyna Stachowicz-Suhs, Natalia Łabędź, Aleksandra Ziemblicka, et al.9216S",CHEMI "Cell Signaling Technology, Danvers, MA, USA",Vitamin D3 Receptor (D2K6W),IgG,"1:125, catalog no: 12550S",CHEMI Antibodies Used for Immunodetection Using Jess Simple Western Method Notes: Near-infrared

(9) Vitamin D inhibits apoptosis in THP-1 cells infected with mycobacterium tuberculosis through TNF signaling pathwayFront ImmunolMay 6, 2025Yusheng Yang, Jiezhong Deng, Pan Liu, Jinyue He, Jiulin Tan, Bo Yu, Yun Bai, Fei Luo, Jianzhong Xu, Zehua Zhangprocedures. Then Immunofluorescence staining was performed for TUNEL (Beyotime, CHN, C1086) and VDR (CST, USA,12550S). The tissues were sliced into 2-mm sections and treated with 0.1% Triton X-100 for 5 min and then blocked

(10) Paricalcitol alleviates intestinal ischemia-reperfusion injury via inhibition of the ATF4-CHOP pathwayFront PharmacolApril 3, 2025Jiawei Zhang, Tingting Liu, Tongqing Xue, Zhongzhi Jiatight junction protein zonula occludens-1 (ZO-1) (82870-7-RR, Proteintech, Wuhan, China; 1:100) or VDR (12550S, Cell Signaling Technology, Danvers, MA, United States; 1:100). This was followed by the addition of

(11) Mechanism of enhancing chemotherapy efficacy in pancreatic ductal adenocarcinoma with paricalcitol and hydroxychloroquineCell Reports MedicineJanuary 21, 2025Ganji Purnachandra Nagaraju, Madhu Sudhana Saddala, Jeremy B. Foote, Ateeq M. Khaliq, Ashiq Masood, Yuvasri Golivi, Dhana Sekhar Reddy Bandi, Sujith Sarvesh, Sudhir Putty Reddy, Jeffrey Switchenko, et al.,Cell signaling technology,Cat#8558; RRID:AB_10831194 Vitamin D3 Receptor,Cell signaling technology,Cat#12550; RRID:AB_2637002 Ki67,Cell signaling technology,Cat#12202; RRID:AB_2620142 "Anti-rabbit IgG, HRP-linked

(12) Vitamin D alleviates HFD-induced hepatic fibrosis by inhibiting DNMT1 to affect the TGFβ1/Smad3 pathwayiScienceDecember 20, 2024Yueqing Liang, Xueyi Jiang, Xinfeng Zhao, Tiantian Tang, Xiuqin Fan, Rui Wang, Mengyi Yang, Kemin Qi, Yi Zhang, Ping Li,Cell Signaling Technology,Cat#9520; RRID: AB_2193207 Rabbit monoclonal anti-VDR,Cell Signaling Technology,Cat#12550; RRID: AB_2637002 Mouse monoclonal anti-CYP27A1,Santa Cruz,Cat#sc-390974 Rabbit polyclonal anti-CYP2R1,"Solarbio,

(13) The vitamin D receptor is essential for the replication of pseudorabies virusmBioDecember 11, 2024Lei Zeng, Shu-Yi Wang, Meng-Hua Du, Bei-Bei Chu, Sheng-Li Minganti-EGFP (50430–2-AP), and anti-PI3K (67071–1-Ig) antibodies were purchased from Proteintech. Anti-VDR (12550), anti-p-p53 (9284), anti-p-AMPKα (50081), anti-AMPKα (2532), anti-p-PI3K (17366), anti-ACACA (3676),

(14) Integration of ATAC-Seq and RNA-Seq Reveals VDR–SELENBP1 Axis Promotes Adipogenesis of Porcine Intramuscular PreadipocytesInternational Journal of Molecular SciencesNovember 22, 2024Jiawei Zhou, Junjing Wu, Tao Yang, Xinyu Zhang, Mu Qiao, Zhong Xu, Yu Zhang, Yue Feng, Tong Chen, Zipeng Li, et al.(1:1000; ABclonal, A2543), GAPDH (1:5000; ABclonal, AC002), and VDR (1:100; Cell Signaling Technology, 12550). Protein expression was detected using an Image Quant LAS4000 mini (GE Healthcare Life Sciences, Piscataway,

(15) Vitamin D Significantly Inhibits Carcinogenesis in the Mogp-TAg Mouse Model of Fallopian Tube Ovarian CancerNutrientsSeptember 30, 2024Omar L. Nelson, Rebecca Rosales, Jane Turbov, Larry G Thaete, Gayathriy Balamayooran, J Mark Cline, J. Wesley Pike, Gustavo C. Rodriguezwestern blotting analysis for p53 (Novacastra NCL-2-p53-CMp, 1:1000), and vitamin D receptor (VDR-Cell Signaling, #12550S) and PAX8 (Proteintech Rosemont, IL, USA 10336-1-AP, 1:500). Actin [(D18C11) cell signaling, cat #12748S]

(16) Discovery of vitexin as a novel VDR agonist that mitigates the transition from chronic intestinal inflammation to colorectal cancerMolecular CancerSeptember 13, 2024Yonger Chen, Jian Liang, Shuxian Chen, Nan Lin, Shuoxi Xu, Jindian Miao, Jing Zhang, Chen Chen, Xin Yuan, Zhuoya Xie, et al.samples, the rest of the sheared chromatin was rotationally incubated with 5 µg of VDR (CST, USA, Cat#12550, 1:50) or IgG at 4 °C overnight. The next day, samples were added to protein A/G dynamic beads and incubated

(17) VDR regulates mitochondrial function as a protective mechanism against renal tubular cell injury in diabetic ratsRedox BiologyApril 1, 2024Hong Chen, Hao Zhang, Ai-mei Li, Yu-ting Liu, Yan Liu, Wei Zhang, Cheng Yang, Na Song, Ming Zhan, Shikun Yangcell injury. 2 Research design and methods Antibodies and reagents: Rabbit IgG antibody to VDR (#12550) and Rabbit IgG antibody to Drp1 (#8570) were purchased from Cell Signaling Technology (Massachusetts,

(18) Epigenetic priming targets tumor heterogeneity to shift transcriptomic phenotype of pancreatic ductal adenocarcinoma towards a Vitamin D susceptible stateCell Death & DiseaseJanuary 26, 2024Bo He, Lauren Stoffel, Clifford Jiajun He, Kumsun Cho, Albert M. Li, Haowen Jiang, Brittany M. Flowers, Kha The Nguyen, Kelly Wen Wang, Audrey Yixin Zhao, et al.supplemented with 3% bovine serum albumin (Equitech-Bio). Anti-VDR antibody (Cell Signaling Technology; 12550s) was diluted at a ratio of 1:1000. Anti-H3K27ac antibody (Active Motif; 39133), anti-H3K4ac antibody

(19) Differential gene regulation by a synthetic vitamin D receptor ligand and active vitamin D in human cellsPLoS OneDecember 13, 2023Miho Iwaki, Yoshiaki Kanemoto, Takahiro Sawada, Koki Nojiri, Tomohiro Kurokawa, Rino Tsutsumi, Kazuo Nagasawa, Shigeaki KatoMotif, Carlsbad, CA, USA) with anti-VDR (rabbit, 1:40; Cell Signaling Technology, Danvers, MA, USA, #12550) and rabbit IgG (mouse, 1:400; Cell Signaling Technology, Danvers, MA, USA, #3900) antibodies[31]. For

(20) Vitamin D(3) Receptors and Metabolic Enzymes in Hen Reproductive TissuesInternational journal of molecular sciencesDecember 3, 2023Hrabia A, Kamińska K, Socha M, Grzesiak M.Species,Vendor,Cat. No,WB Dilution,IF Dilution Anti-VDR,5% NGS,Rabbit,"Cell Signaling, Massachusetts, DA, USA",12550,1:1000,1:50 Anti-PDIA3,5% NGS,Rabbit,"Proteintech, Manchester, UK",15967-1-AP,1:5000,1:200 1α-hydroxylase,5%

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Vitamin D3 Receptor (D2K6W) Rabbit mAb

Vitamin D3 Receptor (D2K6W) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Vitamin D3 Receptor (D2K6W) Rabbit mAb from Cell Signaling Technology

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