GM130 (D6B1) XP ® Rabbit mAb 12480S from Cell Signaling Technology

Product Specs
ItemGM130 (D6B1) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number12480S
Quantity100 µl
ApplicationsW, W-S, IP, IF-IC
ReactivityH, Mk
TargetGM130
HostRabbit
NCBI Full Gene Namegolgin A2
NCBI Gene AliasesDEDHMB, GM130
ClonalityMonoclonal
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD6B1
Molecular Weight130

Questions and Answers

What applications has the GM130 antibody been used for in scientific research?

The GM130 antibody has been shown to work in various applications including Western Blotting, Immunocytochemistry, Immunofluorescence, and Immunoprecipitation, as evidenced by its use in multiple studies.

Can this antibody be used to study Golgi apparatus dynamics in different cell types?

Yes, the GM130 antibody is commonly utilized to investigate Golgi apparatus dynamics, as it is a marker for the Golgi complex. It has been referenced in studies focusing on the secretion of extracellular vesicles and the trafficking of proteins from the Golgi, indicating its relevance in cell biology research.

Is the GM130 antibody effective for detecting protein interactions in extracellular vesicles?

The GM130 antibody has been employed in studies examining the protein composition of extracellular vesicles, demonstrating its utility in analyzing protein interactions and functions related to vesicle secretion and trafficking.

What species does the GM130 antibody react with, and how does this affect its use in experiments?

The GM130 antibody is reactive with Homo sapiens (human) and non-human primates, making it suitable for studies involving these species. This specificity allows researchers to effectively use the antibody in experiments designed to explore Golgi-related processes in human and primate cells.

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Bioengineered extracellular vesicles escape lysosomal degradation and deliver Tet-PKM2 for macrophage immunometabolic reprogramming and periodontitis treatmentBioactive MaterialsMay 1, 2026Wen-Jie Zhang, Bei-Min Tian, Fang Li, Xuan Li, Rui-Xin Wu, Yuan Yin, Jia Wang, Dao-Kun Deng, Yu-Zhe Chen, Hong-Yu Wang, et al.anti-calnexin (1:1000; Cell Signaling Technology, 2433T), anti-GM130 (1:1000; Cell Signaling Technology, 12480T), anti-Hif-1α (1:1000; Cell Signaling Technology, 36169T), and anti-β-actin (1:5000; Proteintech, 66009-1-Ig).

(2) Protocol for the isolation and characterization of extracellular vesicles and particles from human and murine cell linesSTAR ProtocApril 20, 2026Cherlie Lot Sum Yeung, Le Cui, Charlotte Jiaqi Lai, Judy Wai Ping Yam,Cell Signaling Technology,"Cat# 5174, RRID: AB_10622025" "GM130 (rabbit, 1:1000)",Cell Signaling Technology,"Cat# 12480, RRID: AB_2797933" "Goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, HRP - G-21040 (1:10000)",Thermo Fisher Scientific

(3) Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complexSci AdvMarch 20, 2026Aleksandra Dagunts, Hayden Adoff, Brandon Novy, Lamya Ben Ameur, Monica De Maria, Arpiar Saunders, Braden T. Lobingier(Santa Cruz Biotechnology, catalog no. sc-12734; RRID: AB_627263) was used at 1:1000 for Western blots. Anti-GM130 (12480S) was purchased from Cell Signaling Technology and used at 1:500 for immunofluorescence. Donkey anti-mouse

(4) Nitrogen cavitation enables rapid and high-yield preparation of functional cell-membrane-derived vesiclesNanoscale AdvancesMarch 17, 2026Changting Li, Dehua Luo, Can Peng, Qun Luo, Wenjing Xu, Jincheng Zhou, Wen Su, Wei Wu, Yi-Feng Wanganti-nanoluciferase antibody (Promega, cat no. N700A), anti-GM130 antibody (Cell Signaling Technology, cat no. 12480T), anti-lamin B1 antibody (Proteintech, cat no. 12987-1-AP), anti-LAMP1 antibody (Abcam, cat no. ab24170),

(5) Signal peptide peptidase (SPP)- and SPP-like 3 (SPPL3)-dependent shedding of α1,6-fucosyltransferase (FUT8) differentially affects core fucosylationJournal of Biological ChemistryMarch 1, 2026Seita Tomida, Rebeca Kawahara, Kristina Mae Bienes, Yuko Tokoro, Takahiro Yamasaki, Yasuhiko KizukaHealthcare, NA931), HRP-anti-rabbit IgG (GE Healthcare, NA934), rabbit anti-GM130 (Cell Signaling Technology, 12480), Alexa546-anti-mouse IgG (Invitrogen, A10036), and Alexa488-anti-rabbit IgG (Invitrogen, A21206). Mouse

(6) CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assemblyNatureFebruary 25, 2026Alyssa J. Mathiowetz, Emily S. Meymand, Güneş Parlakgül, Niek van Hilten, Emily F. Torres, Leonardo L. Artico, Kirandeep K. Deol, Mike Lange, Stephany P. Pang, Cody E. Doubravsky, et al.antibodies diluted 1:1,000 in 1% BSA in DPBS: rabbit anti-PLIN2 (Abcepta, AP5118c), GM130 (Cell Signaling, 12480), rabbit anti-CLCC1 (Thermo Fisher Scientific, HPA009087), KDEL (Abcam, ab176333), mouse anti-lamin

(7) Osimertinib activates a TGF-β2-dependent secretory program that drives lung adenocarcinoma progressionJournal of Clinical InvestigationFebruary 2, 2026Madhurima Ghosh, Chao Wu, Abhishek Kumar, Monique B. Nilsson, John V. Heymach, Weina Zhao, Jiang Yu, Xin Liu, Na Ding, Shike Wang, et al.(catalog 8685T), p-SMAD (catalog 8828S), vinculin (catalog 4650S), Rab6A (catalog 4879S), GM130 (catalog 12480S), β-actin (catalog 4970S), p65 (catalog 8242T), IL-6 (catalog 12153), p-STAT3 (catalog 9131S), STAT-3

(8) Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STINGCell ReportsJanuary 27, 2026Chen Cheng, Hsiang-Ting Lu, Shan Li, Zhongsheng You,Cell Signaling Technology,Cat# 3724; RRID: AB_1549585 Rabbit mAb anti-GM130,Cell Signaling Technology,Cat# 12480; RRID: AB_2797933 Rabbit anti-mCherry,Cell Signaling Technology,Cat# 43590; RRID: AB_2799246 Rabbit

(9) Breast cancer bone metastasis and bone metastatic cells retain NKG2DLs intracellularly: could this be a strategy to evade immune recognition?Frontiers in Cell and Developmental BiologyJanuary 27, 2026Marta Gomarasca, Paola Maroni, Chiara Verdelli, Laura Gerosa, Martina Faraldi, Alessandro Luzzati, Luca Cannavò, Giuseppe Banfi, Giovanni Lombardi(F-6) (1:500, sc-137242, Santa Cruz Biotechnology), ULBP2 (1:1000, ab275023, AbCam), GM130 (1:10000, 12480, Cell Signaling Technology). β-Actin (1:2000, TA811000, Origene), was used as loading control for total

(10) Cellular and Extracellular MicroRNA Dysregulation in LRRK2-Linked Parkinson’s DiseaseMolecular NeurobiologyJanuary 1, 2026Felix Knab, Jun-Hoe Lee, Raja Nirujogi, Kevin Menden, Luca Braunger, Lambrianna Logarnudi, Benjamin Riebenbauer, Fatma Busra Isik, Anto Praveen Rajkumar, Stefan Czemmel, et al.were used to detect corresponding EV-specific markers. Anti-GM130 antibody (1:1000, Cell Signaling, #12480) was used as a negative control. As a positive detection control, 10 µg of neuronal cell lysates were

(11) Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivityeLifeDecember 3, 2025Hannah Neiswender, Jessica E Pride, Rajalakshmi Veeranan-Karmegam, Phylicia Allen, Grace Neiswender, Avneesh Prabakar, Caili Hao, Xingjun Fan, Graydon B Gonsalvez,Cell Signaling Technologies,60769,1:1000 for western Antibody,Anti-GM130 (rabbit polyclonal),Cell Signaling Technologies,12480,1:1250 for immunofluorescence Antibody,Anti-Cyclin B (rabbit polyclonal),Cell Signaling Technologies,4138T,1:200

(12) Proteomic Exploration of L1CAM+-Extracellular Vesicles from Plasma of Manifest and Prodromal Parkinson's DiseaseInt J Mol SciNovember 28, 2025Mary Xylaki, Avika Chopra, Yevheniia Kyriachenko, Jannik Scherer, Birgit Otte, Mohammed Dakna, Michael Bartl, Sandrina Weber, Sebastian Schade, Christof Lenz, et al.of samples from HC with the following antibodies: anti-Syn1, mouse (610786 BD); anti-GM130, rabbit (12480 Cell Signaling); anti-Annexin V, rabbit (8555 Cell Signaling); anti-CD9, rabbit (13174 Cell Signaling).

(13) TBC1D22B Regulates ER‐to‐Golgi Trafficking via RAB1B Inactivation and Promotes Oncogenic Programs in Breast CancerAdvanced ScienceNovember 1, 2025Flavia Martino, Mariadomenica Lupi, Alessandra Murabito, Fabio Bedin, Giulia Villari, Linda Andreoli, Stefano Freddi, Bronislava Matoskova, Rosa Pennisi, Stella Fontana, et al.anti‐HA‐tag [C29F4 #3724, 1:1000 for immunoblotting (IB), 1:200 for immunofluorescence (IF)], anti‐GM130 (#12480 1:200 for IF).iii) from Santa Cruz: anti‐ACBD3 (sc‐101277 1:1000 for IB), anti‐GAPDH (sc‐32233 1:1000

(14) Morphological Characterization of Plasma-Derived Nanoparticles Isolated by High-Speed Ultracentrifugation: A Scanning Electron Microscopy StudyInternational Journal of Molecular SciencesSeptember 26, 2025Lubov A. Kungurova, Alexander A. Artamonov, Evgeniy A. Grigoryev, Aleksei Yu. Aronov, Olga S. Vezo, Ruslan I. Glushakov, Kirill A. Kondratov(#2171; Cell Signaling Technology), EpCAM (#2626; Cell Signaling Technology, Danvers, MA, USA), GM130 (#12480; Cell Signaling Technology), and CD9 (#13174; Cell Signaling Technology) proteins. The membrane was

(15) Composition of cardiac-derived extracellular vesicles changes with vesicle origin and determines uptakeFrontiers in Cardiovascular MedicineSeptember 15, 2025Sruti Bheri, Jessica R. Hoffman, Hyun-Ji Park, Swarnima Roychowdhury, Felipe Takaesu, Samuel G. Moore, David A. Gaul, Michael E. Davisrabbit anti-BiP (Cat. #3177 T, Cell Signaling Technologies, Danvers, MA), and rabbit anti-GM130 (Cat. #12480S, Cell Signaling Technologies, Danvers, MA). Membranes were then washed three times with PBS-T and were

(16) A role for CASM in the repair of damaged Golgi architecturebioRxivSeptember 11, 2025Seeun Oh, Saif Ullah, Bhaskar Saha, Michael A Mandellphospho-AMPK T172 (2535; 1:1,000 for WB), ATG13 (13468; 1:1,000 for WB), BECN1 (3459; 1:1,000 for WB), GM130 (12480; 1:500 for IF, 1:1,000 for WB), mTOR (2983; 1:500 for IF), phospho-P70 S6K (9205; 1:1,000 for WB), TFEB

(17) Cell line-dependent release of quasi-enveloped hepatitis E virus reveals alternative Golgi-associated egress in the absence of pORF3Frontiers in Cellular and Infection MicrobiologySeptember 5, 2025Nele Gremmel, Mirco Glitscher, Johannes Scholz, Ashish Gadicherla, Reimar Johne, Eberhard Hildt, Paul BecherGlitscher, PEI), mαCD63 (dilution 1:200, abcam, ab59479), rbαGM130 (dilution 1:200, Cell signaling, 12480S), mαTGN46 (dilution 1:100, Thermo, MA3063) or rbαRab11 (dilution 1:100, Cell signaling, 5589T) were

(18) DNAJC13 localization to endosomes is opposed by its J domain and its disordered C-terminusMolecular Biology of the CellSeptember 1, 2025Hayden Adoff, Brandon Novy, Emily Holland, Braden T. Lobingieranti-EEA1 (Cell Signaling, 48453S) and mouse anti-GFP (55494S), rabbit anti-GM130 (Cell Signaling, 12480T, for imaging). From Novus Biologicals, rabbit anti-GFP (Novus Biologicals, NB600-308, for imaging).

(19) STING inhibits LINE-1 retrotransposition through sorting ORF1p to lysosomes for degradationEMBO ReportsAugust 18, 2025Yu Huang, Fengwen Xu, Lingwa Wang, Shan Mei, Fei Zhao, Liming Wang, Yu Xie, Liang Wei, Yamei Hu, Zhao Gao, et al.anti-TBK1 antibody,Cell Signaling Technology,3013 Rabbit anti-GM130 antibody,Cell Signaling Technology,12480 Rabbit anti-ATG5 antibody,Cell Signaling Technology,12994 Mouse anti-LAMP1 antibody,BioLegend,328602 Rabbit

(20) An Automated Imaging Method for Quantification of Changes to the Endomembrane System in Mammalian Spheroid ModelsBIO-PROTOCOLJune 5, 2025Margaritha M. Mysior, Jeremy C. SimpsonT9284-500ml); store at RT 11. Rabbit anti-GM130 (clone: D6B1) (Cell Signalling Technologies, catalog number: 12480); store at -20 °C 12. Mouse anti-EEA1 (clone: 14/EEA1) (BD Biosciences, catalog number: 610457); store

Be the First to Write a Review!

GM130 (D6B1) XP ® Rabbit mAb

GM130 (D6B1) XP ® Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (189)

Reviews

GM130 (D6B1) XP ® Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map