MacroH2A1.1 (D5F6N) Rabbit mAb 12455S from Cell Signaling Technology

Product Specs
ItemMacroH2A1.1 (D5F6N) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12455S
Quantity100 µl
ApplicationsW, IF-IC
ReactivityH, M, R
TargetH2A1.1
HostRabbit
NCBI Full Gene NamemacroH2A.1 histone
NCBI Gene AliasesH2A.y, H2A/y, H2AF12M, H2AFY, MACROH2A1.1, mH2A1, macroH2A1.2
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
CloneD5F6N
Molecular Weight40

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Histone-based liquid biopsy discriminates between myelodysplastic syndrome and solid malignanciesClinical EpigeneticsNovember 21, 2025Desislava K. Tsoneva, Diana Buzova, Salvatore Daniele Bianco, Ilina Micheva, Merlin Efraim, Nikol Daskalova, Igor Resnick, Maria Teneva, Trifon Chervenkov, Nikolay Vladimirov Conev, et al.LS-B6334-125; polyclonalH4; Abcam, Ab31830; monoclonal",II,"macroH2A1.1; Cell Signaling Technology, 12455S; monoclonal" "H2B; BioLegend, 606,302; monoclonalH3; LS Bio, LS-B6334-125; polyclonalH4; Abcam, Ab31830;

(2) Ubiquitination of the histone variant mH2A1.2 prevents toxic RAD18 accumulation at a subset of genomic loci upon replication stressMolecular CellAugust 21, 2025Maxime Galloy, Andréanne Blondeau, Élodie Vion, Daein Kim, Collin A. Bakker, Vincent Gaggioli, Mélissa Thomas, Élise G. Lavoie, Isabelle Marois, Alberto David Delgado Monterroso, et al.anti-6×His (631212; Takara Bio Inc.), anti-macro-H2A1.2 (MABE61; MilliporeSigma), anti-macroH2A1.1 (12455; Cell Signaling Technology), anti-mH2A2 (Ab242357; Abcam), anti-Rad18 (D2B8) XP (9040; Cell Signaling Technology

(3) Detection of cell-free histones in the cerebrospinal fluid of pediatric central nervous system malignancies by imaging flow cytometryFrontiers in Molecular BiosciencesNovember 1, 2023Diana Buzova, Jan Frohlich, Danica Zapletalova, Marco Raffaele, Oriana Lo Re, Desislava K. Tsoneva, Jaroslav Sterba, Jan Cerveny, Manlio Vinciguerrasecondary antibodies. First staining set. Primary antibodies: anti-macroH2A1.1 (Cell Signaling Technology, 12455S, United States), anti-histone H2B (Abcam, Ab134211, United States), anti-histone H4 (Abcam, Ab31830,

(4) HSV-1 exploits host heterochromatin for nuclear egressJournal of Cell BiologySeptember 4, 2023Hannah C. Lewis, Laurel E. Kelnhofer-Millevolte, Mia R. Brinkley, Hannah E. Arbach, Edward A. Arnold, Saskia Sanders, Jens B. Bosse, Srinivas Ramachandran, Daphne C. Avgousti37264,"WB (1:1,000)IF (1:500)CUT&Tag (1:100)" Rabbit anti-macroH2A1.1,Cell Signaling Technology,Cat: 12455S,"WB (1:1,000)" Mouse anti-macroH2A1.2,Cell Signaling Technology,Cat: 4827S,"WB (1:1,000)" Rabbit anti-macroH2A2,Active

(5) Aging, Plasminogen Activator Inhibitor 1, Brain Cell Senescence, and Alzheimer's DiseaseAging and diseaseApril 1, 2023Jiang CS, Rana T, Jin LW, Farr SA, Morley JE, Qin H, Liu G, Liu RM.formalin-fixed tissue slides with rabbit monoclonal antibody to MacroH2A1.1 (Cell signaling, Cat No 12455, Danvers, MA USA), a cell senescence marker, and mouse monoclonal antibody to glial fibrillary acidic

(6) Next-generation proteomics of serum extracellular vesicles combined with single-cell RNA sequencing identifies MACROH2A1 associated with refractory COVID-19Inflammation and regenerationNovember 30, 2022Kawasaki T, Takeda Y, Edahiro R, Shirai Y, Nogami-Itoh M, Matsuki T, Kida H, Enomoto T, Hara R, Noda Y, et al.the following proteins were used: MACROH2A1 (no. 8551; Cell Signaling Technology), MACROH2A1.1 (no. 12455; Cell Signaling Technology), MACROH2A1.2 (no. 4827; Cell Signaling Technology), p-p65 (no. 3033; Cell Signaling Technology

(7) Spatial and Functional Organization of Human Papillomavirus Replication Foci in the Productive Stage of InfectionmBioDecember 21, 2021Khurana S, Markowitz TE, Kabat J, McBride AA.at 37°C for 1 h. Primary antibodies used for IF were macroH2A1.1 rabbit monoclonal (Cell Signaling, 12455; dilution, 1:100), macroH2A1.2 mouse monoclonal (Millipore, MABE61; dilution, 1:100), H2A rabbit polyclonal

(8) Poly(ADP-ribose) binding and macroH2A mediate recruitment and functions of KDM5A at DNA lesionsThe Journal of cell biologyJuly 5, 2021Kumbhar R, Sanchez A, Perren J, Gong F, Corujo D, Medina F, Devanathan SK, Xhemalce B, Matouschek A, Buschbeck M, et al.anti-Flag (F1804; Sigma-Aldrich), rabbit anti-macroH2A1 (Ab37264; Abcam), rabbit anti-macroH2A1.1 (12455S [D5F6N]; Cell Signaling Technology), mouse anti-macroH2A1.2 (MABE61 [14G7]; Sigma-Aldrich), rabbit IgG

(9) Epigenetic Regulation of DNA Repair Pathway Choice by MacroH2A1 Splice Variants Ensures Genome StabilityMolecular cellSeptember 3, 2020Sebastian R, Hosogane EK, Sun EG, Tran AD, Reinhold WC, Burkett S, Sturgill DM, Gudla PR, Pommier Y, Aladjem MI, et al.following antibodies were used for western blot: α-macroH2A1.2 (Millipore, MABE61), α-macroH2A1.1 (CST, 12455), α-Lig3 (GeneTex, GTX103172), α-FLAG (Agilent, 200472), α-PCNA (Santa Cruz, sc-56), α-H2B (Abcam, ab1790),

(10) Id1 expression in kidney endothelial cells protects against diabetes-induced microvascular injuryFEBS open bioAugust 1, 2020Sharma S, Plotkin M.CA, USA), rabbit anti‐phospho‐histone γH2AX and macroH2A.1.1 (Cell Signaling Technology #9718 and #12455, respectively), rat anti‐mouse CD31 (clone MEC 13.3, #557355; BD Biosciences, San Jose, CA, USA), and

(11) Targeting PML in triple negative breast cancer elicits growth suppression and senescenceCell death and differentiationApril 1, 2020Arreal L, Piva M, Fernández S, Revandkar A, Schaub-Clerigué A, Villanueva J, Zabala-Letona A, Pujana M, Astobiza I, Cortazar AR, et al.anti-p27[Kip1] antibody 1:100 dilution (Cat: 610242, BD Biosciences) and anti-macroH2A1.1 antibody 1:100 (Cat: 12455, Cell Signaling) were added ON (4 °C) in goat serum. Cover slips were washed with PBS three times and

(12) MacroH2A1 Regulation of Poly(ADP-Ribose) Synthesis and Stability Prevents Necrosis and Promotes DNA RepairMolecular and cellular biologyDecember 11, 2019Ruiz PD, Hamilton GA, Park JW, Gamble MJ.Coverslips were ﬁxed and washed as described above and stained with macroH2A1.1 antibody (Cell Signaling; 12455S; 1:500) overnight and with goat anti-rabbit IgG conjugated to Alexa Fluor 488 (Life Technologies; A-11034;

(13) CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicityNature communicationsSeptember 5, 2019Sang Y, Li Y, Zhang Y, Alvarez AA, Yu B, Zhang W, Hu B, Cheng SY, Feng H.(1:1000, #2276), anti-tubulin (1:1000, #2148), anti-His (1:1000, #2365), anti-macroH2A1.1 (1:1000, #12455), anti-macroH2A1.2 (1:1000, #4827), and anti-HA (1:1000, #3724) antibodies (Cell Signaling Technology);

(14) Histone Variant MacroH2A1 Plays an Isoform-Specific Role in Suppressing Epithelial-Mesenchymal TransitionScientific ReportsDecember 1, 2018Dayle Q. Hodge, Jihong Cui, Matthew J. Gamble, Wenjun GuoBDB610182), anti-Vimentin (Cell Signaling Technology – 5741S), anti- macroH2A1.1 (Cell Signaling Technology – 12455S), anti- macroH2A1.2 (Cell Signaling Technology – 4827S) or anti-Histone H3 (Cell Signaling Technology

(15) The histone variant macroH2A confers functional robustness to the intestinal stem cell compartmentPLOS ONESeptember 21, 2017Ryan James Cedeno, Angela Nakauka-Ddamba, Maryam Yousefi, Stephanie Sterling, Nicolae Adrian Leu, Ning Li, John R. Pehrson, Christopher Joachim Lengnerantibodies and dilutions used were as follows: macroH2A1 (Abcam Ab37264, 1:200), macroH2A1.1 (CST #12455, 1:200), tdTomato (ClonTech 632392, 1:200), Ki67 (Abcam Ab15580, 1:200), Lysozyme C (Santa Cruz sc-27958,

(16) Histone macroH2A1.2 promotes metabolic health and leanness by inhibiting adipogenesisEpigenetics & ChromatinDecember 1, 2016Valerio Pazienza et al.Primary antibodies were as follows: anti-GFP (Abcam, ab13970) anti-macroH2A1.1 (Cell Signaling, Cat 12455), anti-macroH2A1.2 (Cell Signaling, Cat. 4827), anti-AKT (Cell Signaling, Cat. 9272), anti-phosphoAKT-Ser473

(17) QKI5-mediated alternative splicing of the histone variant macroH2A1 regulates gastric carcinogenesisOncotargetMay 31, 2016Li F, Yi P, Pi J, Li L, Hui J, Wang F, Liang A, Yu J.repeated three times. Chromatin immunoprecipitation (ChIP) assay Anti-mH2A1.1 antibody (Cell Signaling, 12455) and rabbit isotype control IgG (Abcam, ab27472) were used for ChIP studies. HGC-27 cells were collected

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MacroH2A1.1 (D5F6N) Rabbit mAb

MacroH2A1.1 (D5F6N) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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MacroH2A1.1 (D5F6N) Rabbit mAb from Cell Signaling Technology

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