Indirect immunostaining of PFA fixed rat hippocampus neurons with anti-GAT 3 (dilution 1 : 500; red) and mouse anti-synapsin 1 (cat. no. 106 001, dilution 1 : 1000; green). Nuclei have been visualized by DAPI staining (blue).

Indirect immunostaining of mouse cerebellum sections (dilution 1 : 750; red). The animals were perfusion fixed with 4 % formaldehyde and 0.1 % glutaraldehyde (Danbolt et al., 1998).

Courtesy: Yun Zhou and Niels Christian Danbolt
Department of Anatomy
Institute of Basic Medical Sciences
University of Oslo

Indirect immunolabeling of PFA fixed, paraffin embedded mouse cerebellum with rabbit anti-GAT 3 (dilution 1 : 500; red) and chicken anti-parvalbumin (cat. no. 195 006, dilution 1 : 500; green). Nuclei have been visualized by DAPI staining (blue).