RFP Antibody 200-301-379 from Rockland Immunochemicals, Inc.

Product Specs
ItemRFP Antibody
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number200-301-379
Quantity100 µg
ApplicationsELISA,Flow Cytometry,IF Microscopy,Immunoprecipitation,Western Blot
Concentration1.02 mg/ml
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
IsotypeIgG2a kappa
Clone8E5.G7
HostMouse
ImmunogenThe immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma
ClonalityMonoclonal
Gene NameDsRed
ReactivityRFP, mCherry, rRFP
TargetRFP
Antibody TypePrimary
StorageStore anti-RFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Note: Monoclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA and Western blot, and is suitable for use in FISH, IF, IHC, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely RegenerativeJournal of Cardiovascular Development and DiseaseApril 7, 2023Hirofumi Watanabe, Ge Tao, Peiheng Gan, Baylee C. Westbury, Kristie D. Cox, Kelsey Tjen, Ruolan Song, Glenn I. Fishman, Takako Makita, Henry M. Sucovscreen was used. Staining was performed with the following primary antibodies: rabbit anti-RFP (tdT) (200-301-379; Rockland, Pottstown, PA, USA, at 1:200); chicken anti-GFP (ab13970; Abcam, Cambridge, UK, at 1:500);

(2) TRPC3 channel gating by lipids requires localization at the ER/PM junctions defined by STIM1Journal of Cell BiologyMay 2, 2022Haiping Liu, Wei-Yin Lin, Spencer R. Leibow, Alexander J. Morateck, Malini Ahuja, Shmuel Muallemanti-myc antibodies (2276S; Cell Signalling Technologies), and VAPA and VAPB with anti-RFP antibodies (200-301-379; Rockland). Non-biotinylated Co-IPs were performed using GE HealthCare Protein G Sepharose 4 Fast Flow

(3) Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual CortexCellsApril 20, 2022Henriques VJ, Chiavegato A, Carmignoto G, Gómez-Gonzalo M.rabbit polyclonal, Abcam, ab11427) and monoclonal anti-RFP (RRID:AB_2611063, 1:1000, mouse, Rockland, 200-301-379) with donkey Alexa Fluor 488 conjugated anti-rabbit (RRID:AB_2535792, 1:500, Thermofisher Scientific

(4) Nutrients and pheromones promote insulin release to inhibit courtship driveScience advancesMarch 11, 2022Zhang L, Guo X, Zhang W.subsequent immunostaining was performed. Rabbit anti-DILP2 (1:1000), mouse anti-RFP (1:500; Rockland, 200-301-379), Alexa Fluor 488 goat anti-rabbit immunoglobulin G (IgG), and Alexa Fluor 647 goat anti-mouse IgG were

(5) Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunityCancer cellSeptember 13, 2021Hutton C, Heider F, Blanco-Gomez A, Banyard A, Kononov A, Zhang X, Karim S, Paulus-Hock V, Watt D, Steele N, et al.C-11,Biolegend,628602 Anti-mouse VIM 154Sm clone D21H3,Fluidigm,3154014A Anti-mouse RFP clone 8E5.G7,Rockland Inc,200-301-379 Anti-mouse DES clone Y66,Abcam,ab271829 Anti-mouse aSMA clone 1A4,Abcam,ab240654 Anti-human/mouse

(6) Homeostatic synaptic scaling establishes the specificity of an associative memoryCurrent biology : CBJune 7, 2021Wu CH, Ramos R, Katz DB, Turrigiano GG.monoclonal anti-NeuN (A60),Millipore,MAB-377; RRID: AB_2298772 Mouse monoclonal anti-RFP (8E5.G7),Rockland,200-301-379; RRID: AB_2611063 Rabbit monoclonal anti-c-FOS (9F6),Cell Signaling Technology,2250; RRID: AB_2247211 Rat

(7) Salamander-Eci: An optical clearing protocol for the three-dimensional exploration of regenerationDevelopmental dynamics : an official publication of the American Association of AnatomistsJune 1, 2021Subiran Adrados C, Yu Q, Bolaños Castro LA, Rodriguez Cabrera LA, Yun MH.

(8) Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbirdNature communicationsMay 11, 2021Xiao L, Merullo DP, Koch TMI, Cao M, Co M, Kulkarni A, Konopka G, Roberts TF.rabbit anti-RFP (1:1000, mCherry, 600-401-379, Rockland, PA, USA), mouse anti-RFP (1:1000, mCherry, 200-301-379, Rockland, PA, USA), rabbit anti-GFP (1:2000, A11122, Invitrogen, CA, USA), chicken anti-GFP (1:1000,

(9) An inhibitory hippocampal-thalamic pathway modulates remote memory retrievalNature neuroscienceMay 1, 2021Vetere G, Xia F, Ramsaran AI, Tran LM, Josselyn SA, Frankland PW.same brains were blocked and then incubated with mouse anti-RFP (1:1,000 dilution; Rockland Cat. no. 200–301-379 RRID: AB_2611063) and rabbit anti-VGAT (1:500 dilution; EMD Millipore Cat. no. AB2257 RRID: AB_1587623)

(10) Increased Callosal Connectivity in Reeler Mice Revealed by Brain-Wide Input Mapping of VIP Neurons in Barrel CortexCerebral cortex (New York, N.Y. : 1991)February 5, 2021Hafner G, Guy J, Witte M, Truschow P, Rüppel A, Sirmpilatze N, Dadarwal R, Boretius S, Staiger JF.

(11) GABAergic neurons are susceptible to BAX-dependent apoptosis following isoflurane exposure in the neonatal periodPloS oneJanuary 1, 2021Slupe AM, Villasana L, Wright KM.

(12) Astrocytes shape the plastic response of adult cortical neurons to vision lossGliaOctober 1, 2020Hennes M, Lombaert N, Wahis J, Van den Haute C, Holt MG, Arckens L.

(13) Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent mannereLifeApril 28, 2020Guan Z, Quiñones-Frías MC, Akbergenova Y, Littleton JT.anti-Golgin84 at 1:50 (Golgin84 12–1; DSHB), mouse anti-RAB7 at 1:10 (Rab7; DSHB), mouse anti-RFP at 1:1000 (200-301-379; Rockland, Limerick, PA, USA) mouse anti-SYX1 at 1:100 (8C3; DSHB), rabbit anti-CPX at 1:5000 (Huntwork

(14) Npas1⁺-Nkx2.1⁺ Neurons Are an Integral Part of the Cortico-pallido-cortical LoopThe Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 22, 2020Abecassis ZA, Berceau BL, Win PH, García D, Xenias HS, Cui Q, Pamukcu A, Cherian S, Hernández VM, Chon U, et al.

(15) Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit MotifsCell reportsSeptember 24, 2019Hafner G, Witte M, Guy J, Subhashini N, Fenno LE, Ramakrishnan C, Kim YS, Deisseroth K, Callaway EM, Oberhuber M, et al.

(16) Lmx1b is required at multiple stages to build expansive serotonergic axon architectureseLifeJuly 29, 2019Donovan LJ, Spencer WC, Kitt MM, Eastman BA, Lobur KJ, Jiao K, Silver J, Deneris ES.all co-stains with TdTomato a mouse anti-RFP (1:200; ab65856, Abcam) or a mouse anti-RFP (1:200; p/n 200-301-379, Rockland) primary antibody was used. Secondary antibodies were used at 1:500; goat anti-rabbit or mouse,

(17) A new Elf5^Cre ^ERT ^2- ^GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissuesFEBS lettersMay 1, 2019Singh S, Elenio E, Leu NA, Romano RA, Vaughan AE, DeRiso J, Surendran K, Chakrabarti R.

(18) An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorantsThe EMBO journalMarch 15, 2019Bragado Alonso S, Reinert JK, Marichal N, Massalini S, Berninger B, Kuner T, Calegari F.,147 021 GFP,1:400,Rockland,600‐101‐215 Mash1,1:100,BD Biosciences,556604 NeuN,1:250,Millipore,MAB377 RFP,"1:2,000",Rockland,200‐301‐379 S100β,"1:1,000",Abcam,ab14688 TH,1:250,Millipore,MAB5280 VIAAT,1:400,Synaptic Systems,131 004 Secondary

(19) Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated DeficitsCell reportsMarch 5, 2019Katrancha SM, Shaw JE, Zhao AY, Myers SA, Cocco AR, Jeng AT, Zhu M, Pittenger C, Greer CA, Carr SA, et al.minutes at room temperature. Cells were incubated with primary antibodies, mouse anti–RFP (Rockland 200–301–379; 1:1,000) and a rabbit antibody generated in–house to the DH portion of the TRIO GEF2 domain (DH2, 1:50)

(20) Mild Traumatic Brain Injury Induces Structural and Functional Disconnection of Local Neocortical Inhibitory Networks via Parvalbumin Interneuron Diffuse Axonal InjuryCerebral cortex (New York, N.Y. : 1991)May 1, 2018Vascak M, Jin X, Jacobs KM, Povlishock JT.

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RFP Antibody from Rockland Immunochemicals, Inc.

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Rockland Immunochemicals, Inc.

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RFP Antibody from Rockland Immunochemicals, Inc.

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